Radiation cytogentics of the yellow-fever mosquito Aedes aegypti and the plant genus Collinsia. Final report, April 1967--September 1977

Abstract

Final report on ERDA Supported Research Project on "Radiation Cytogentics of the yellow-fever mosquito Aedes degypti and the plant genus Collinsia.

A technique for preparing polytene chromosomes from Aedes aegypti (Diptera, Culicinae)

“…Several problems have been already suggested as causal of the low quality of polytene chromosomes preparations which could be suitable for analysis in Aedes and Culex: (1) Sutton (1942) suggested the presence of weak points, which can be assumed now as being heterochromatic areas where the chromosomes break easily (Semeshin et al 2001); (2) the great length of chromosome arms (Kitzmiller 1963) should influence chromatic interactions; (3) the inter-and intra-chromosomal connections, or ectopic pairing (French et al 1962, Verma et al 1987 resulting from regions of highly repetitive DNA (Rai & Black IV 1999); (4) surface adhesions (Rai 1967apud Sharma et al 1978) that have been observed in Anopheles funestus and is dependent on β-heterochromatin (Sharakhov et al 2001) and (5) asynapsis observed in the polytene complement (Zambetaki et al 1998). …”