Radiation and heat sensitivity of cells from two slowly growing human melanoma xenografts

Rofstad, Einar K.; Wahl, A.; Brustad, Tor

doi:10.1038/bjc.1984.117

Cited by 9 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Breast cancer is a relatively radiosensitive tumor and, in general, may be more radiosensitive than NSCLC. 20,21 Therefore, it is not surprising that breast cancers of a few centimeters were controlled by our high-dose irradiation method. Even when the tumor develops local recurrence, salvage surgery may be feasible; indeed, one of our patients underwent salvage breast-conserving surgery successfully.…”

Section: Discussionmentioning

confidence: 97%

Definitive Radiotherapy With SBRT or IMRT Boost for Breast Cancer: Excellent Local Control and Cosmetic Outcome

Shibamoto

Murai

Suzuki

et al. 2018

Technol Cancer Res Treat

View full text Add to dashboard Cite

The gold standard for breast cancer treatment is surgery, but many women may desire to avoid surgery if possible. The purpose of this study was to evaluate whether breast cancer could be cured with modern sophisticated radiation techniques with good cosmetic outcome. We have treated 18 patients with operable breast cancer by conventional whole-breast irradiation followed by stereotactic body radiotherapy (primary tumor only) or intensity-modulated radiotherapy (tumor plus axillary nodes) boost. The planned doses were 50 Gy in 25 fractions, 18 to 25.5 Gy in 3 fractions, and 20 Gy in 8 fractions, respectively, for the 3 modalities. Stereotactic body radiotherapy was delivered with 7 to 9 coplanar and noncoplanar fixed beams, and intensity-modulated radiotherapy was given by tomotherapy. Chemotherapy and/or hormone therapy was used depending on the stage and receptor status. In 9 recent patients, hydrogen peroxide was intratumorally injected twice a week before whole-breast irradiation. All treatments were well tolerable and there were no grade ≥3 toxicities. With a median follow-up period of 35 months (range, 8-120 months), only 1 patient developed local recurrence and 2 patients developed distant metastasis. Overall survival, progression-free survival, and local control rates were 93%, 85%, and 92%, respectively, at 3 years. In 50% of the patients, the irradiated breast became better rounded, and the position of the nipple of the irradiated breast became ≥1 cm higher compared to that of the unirradiated breast. Thus, the treated breasts may be more aesthetically favorable than before irradiation in these patients. This may become a treatment option for patients with operable breast cancer.

show abstract

Section: Discussionmentioning

confidence: 97%

Definitive Radiotherapy With SBRT or IMRT Boost for Breast Cancer: Excellent Local Control and Cosmetic Outcome

Shibamoto

Murai

Suzuki

et al. 2018

Technol Cancer Res Treat

View full text Add to dashboard Cite

show abstract

“…Light and electron-microscopic examinations showed that the histological appearance of the xenografts in these passages was similar to that of the metastases from which they were derived. The growth characteristics of subcutaneous flank tumours of the xenografts, approximately 200 mm3 in size, have been reported previously Rofstad, Wahl & Brustad, 1984).…”

Section: Xenograftsmentioning

confidence: 67%

Growth Characteristics of Human Melanoma Multicellular Spheroids In Liquid‐Overlay Culture: Comparisons With the Parent Tumour Xenografts

et al. 1986

View full text Add to dashboard Cite

The growth characteristics of multicellular spheroids, derived from human melanoma xenografts and cultivated in liquid-overlay culture, were studied and compared with those of the parent tumours. Six of the seven melanomas investigated formed spheroids, which grew exponentially up to a volume of 1-2 x lo7 pm3 (a diameter of 270-340 pm) before the growth rate tapered off. The morphology of the spheroids varied considerably among the melanomas; some spheroids grew as densely packed, spherical structures of cells whereas others were loosely packed and showed an irregular shape. Central necrosis developed when the spheroids attained a diameter of 150-200 ,urn. The histological and cytological appearance of the spheroids was remarkably similar to that of the parent xenograft in five of the six cases. The sixth melanoma contained two subpopulations with distinctly different DNA content, one of which was predominant in the spheroids, the other in the tumours. This gave rise to clear histological and cytological differences. The volume-doubling time of the spheroids during the exponential growth phase ranged from 1.7 k 0.2 to 2.7 0.4 days and the fraction of cells in S from 13 & 1 to 28 & 2%. The volume-doubling time decreased with increasing fraction of cells in S, indicating that the differences in growth rate were due mainly to differences in the growth fraction or to differences in the duration of G,. The spheroid volume-doubling times did not correlate with those of the parent xenografts (T, = 4.2-22.5 days at V = 200 mm3), possibly because the cell loss factors of the xenografts were large and varied among the melanomas. The fractions of cells in G,/G, S and G, + M in the spheroids and the xenografts did not correlate either, but were found to be within the same narrow ranges in the spheroids and the xenografts4.e. 50-80% (G,/G,,), 10-30% (S) and 10-20% (G, + M).Multicellular spheroids are an in v i m tumour model system representing an intermediate level of complexity between monolayer cell cultures and solid tumours (Sutherland el al., 1970;Sutherland & Durand, 1976). Spheroids resemble tumours in that the cells grow in a three-dimensional structure which facilitates intercellular communication (Durand &

show abstract

Fractionation sensitivity (α/β ratio) of human melanoma xenografts

Rofstad

1994

Radiotherapy and Oncology

View full text Add to dashboard Cite

Radiation and heat sensitivity of cells from two slowly growing human melanoma xenografts

Abstract: Summary The radiation and heat sensitivity of cells from two melanin-rich, slowly growing human melanoma xenografts (B.E. and R.A.)

Cited by 9 publications

References 30 publications

Definitive Radiotherapy With SBRT or IMRT Boost for Breast Cancer: Excellent Local Control and Cosmetic Outcome

Definitive Radiotherapy With SBRT or IMRT Boost for Breast Cancer: Excellent Local Control and Cosmetic Outcome

Growth Characteristics of Human Melanoma Multicellular Spheroids In Liquid‐Overlay Culture: Comparisons With the Parent Tumour Xenografts

Fractionation sensitivity (α/β ratio) of human melanoma xenografts

Contact Info

Product

Resources

About