Radial Immunodiffusion of Spinal Fluid: Diagnostic Value in Multiple Sclerosis

Berner, Jerome J.; Ciemins, Vilnis A.; Schroeder, Edmund F.

doi:10.1093/ajcp/58.2.145

Cited by 12 publications

(1 citation statement)

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“…1973, M34, p. 79), are commonly used in clinical laboratory determinations of CSF IgG and albumin. Tables of normal values have been compiled, and abnormal IgG and albumin concentrations and IgG/albumin ratios in neurological disorders have been described (3,6,10,13).…”

mentioning

confidence: 99%

Application of fluoroimmunoassay to cerebrospinal fluid immunoglobulin G and albumin

Stevens¹,

Elmendorf²,

Gourlay³

et al. 1979

J Clin Microbiol

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Special solid-phase fluoroimmunoasssay protocols were used to measure the amount of immunoglobulin G (IgG) and albumin in 1,511 samples of cerebrospinal fluid. The fluoroimmunoassay is an inhibition test conducted on the surface of a plastic probe, whose fluorescence is inversely related to the concentration of spinal fluid IgG or albumin. A microcomputer interfaced with the fluorometer calculates the sample IgG or albumin from a calibration curve based on standard cerebrospinal fluid values. The test and interpretation take less than 90 min. Correlation coefficients for over 100 cerebrospinal fluid samples tested by both fluoroimmunoassay and radial immunodiffusion were: IgG, 0.90 (slope, 1.04); albumin, 0.95 (slope, 0.99). The within-run precision (coefficient of variation) was: IgG, 4.4%; albumin, 6.3%. Run-to-run precision on a midrange sample was: IgG, 7.7%, albumin, 11.7%. These findings establish the simplicity, speed, and precision of the modified fluoroimmunoassay system for specific cerebrospinal fluid proteins.

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mentioning

confidence: 99%

Application of fluoroimmunoassay to cerebrospinal fluid immunoglobulin G and albumin

Stevens¹,

Elmendorf²,

Gourlay³

et al. 1979

J Clin Microbiol

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Active Multiple Sclerosis

Sears¹

1978

Arch Neurol

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Multiple sclerosis: Diagnostic usefulness of cerebrospinal fluid

Johnson¹,

Nelson²

1977

Annals of Neurology

103

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Early, atypical, or progressive cases of multiple sclerosis (MS) may be hard to diagnose. Until recently, assays of the most common abnormalities in cerebrospinal fluid were not available in clinical diagnostic laboratories, but now they can be done with relative ease and adequate standardization. With the newer techniques the CSF is abnormal in more than 90% of clinically definite cases of MS, and determination of such changes can be a major aid in diagnosis. The most common CSF abnormalities are discussed: elevation of immunoglobulin G(IgG), expressed as percentage of total protein; elevation of the IgG/albumin index; and presence of oligoclonal IgG bands. Not only does assessment of these CSF proteins provide an improved aid to diagnosis, but their study may furnish important clues to the cause and pathogenesis of MS as well.

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Radial Immunodiffusion of Spinal Fluid: Diagnostic Value in Multiple Sclerosis

Cited by 12 publications

References 0 publications

Application of fluoroimmunoassay to cerebrospinal fluid immunoglobulin G and albumin

Application of fluoroimmunoassay to cerebrospinal fluid immunoglobulin G and albumin

Active Multiple Sclerosis

Multiple sclerosis: Diagnostic usefulness of cerebrospinal fluid

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