Radial Glial Cell Line C6-R Integrates Preferentially in Adult White Matter and Facilitates Migration of Coimplanted Neurons in Vivo

Hormigo, Adı́lia; McCarthy, Maria; Nothias, Jean Manuel; Hasegawa, Koichi; Huang, Wen Cheng; Friedlander, David R.; Fischer, Itzhak; Fishell, Gord; Grumet, Martin

doi:10.1006/exnr.2000.7620

Cited by 14 publications

(4 citation statements)

References 46 publications

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“…Co‐transplantation of RG‐like cells with neural progenitors into adult murine brain improves transplanted cell engraftment (Hormigo et al, 2001). In the present work, we show that ES cell‐derived RG cells express similar cell markers as their in vivo counterparts and in vitro generation of RG cells is independent of the differentiation conditions.…”

Section: Discussionmentioning

confidence: 99%

Further characterization of embryonic stem cell‐derived radial glial cells

Liour

Kraemer

Dinkins

et al. 2005

Glia

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Previously, we showed that radial glia-like (RG) cells differentiated from embryonic stem (ES) cells after retinoic acid induction (Liour and Yu, 2003: Glia 42:109-117). In the present study, we demonstrate that the production of RG cells from ES cells is independent of the neural differentiation protocol used. These ES cell-derived RG (ES-RG) cells are similar in morphology to RG cells in vivo and express several characteristic RG cell markers. The processes of these ES-RG cells are organized into radial arrays similar to the RG scaffold in developing CNS. Expression of Pax6, along with other circumstantial data, suggests that at least some of these ES-RG cells are neural progenitors. The progression of neurogenesis into gliogenesis during the in vitro neural differentiation of ES cells recapitulates the in vivo developmental process. The identification of two cell surface markers, SSEA-1 and GM1, on both the native embryonic RG cells and ES-RG cells, may facilitate purification of radial glial cells for future studies and cell therapy. Overall, our study suggests that differentiation of radial glial cells is a common pathway during the neural differentiation of ES cells.

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Section: Discussionmentioning

confidence: 99%

Further characterization of embryonic stem cell‐derived radial glial cells

Liour

Kraemer

Dinkins

et al. 2005

Glia

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“…NIH 3T3 cells (ATCC, Manassas, VA) and GFP-expressing C6-R radial glial cell line derived from C6 glioma cells (kindly provided by Dr. Martin Grumet, Rutgers University) [51] were cultured in DMEM (Hyclone, Logan UT) supplemented with 10% bovine calf serum (Hyclone, Logan, UT) and 1% penicillin/streptomycin. PC12 cells were cultured in RPMI 1640 Basal medium supplemented with 10% horse serum, 5% fetal bovine serum, and 1 % penicillin/streptomycin and differentiated using RPMI 1640 basal media supplemented with 1% horse serum and 100 ng/ml NGF.…”

Section: Methodsmentioning

confidence: 99%

Nerve Guidance by a Decellularized Fibroblast Extracellular Matrix

et al. 2017

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Spinal cord and peripheral nerve injuries require the regeneration of nerve fibers across the lesion site for successful recovery. Providing guidance cues and soluble factors to promote neurite outgrowth and cell survival can enhance repair. The extracellular matrix (ECM) plays a key role in tissue repair by controlling cell adhesion, motility, and growth. In this study, we explored the ability of a mesenchymal ECM to support neurite outgrowth from neurons in the superior cervical ganglia (SCG). Length and morphology of neurites extended on a decellularized fibroblast ECM were compared to those on substrates coated with laminin, a major ECM protein in neural tissue, or fibronectin, the main component of a mesenchymal ECM. Average radial neurite extension was equivalent on laminin and on the decellularized ECM, but contrasted with the shorter, curved neurites observed on the fibronectin substrate. Differences between neurites on fibronectin and on other substrates were confirmed by fast Fourier transform analyses. To control the direction of neurite outgrowth, we developed an ECM with linearly aligned fibril organization by orienting the fibroblasts that deposit the matrix on a polymeric surface micropatterned with a striped chemical interface. Neurites projected from SCGs appeared to reorient in the direction of the pattern. These results highlight the ability of a mesenchymal ECM to enhance neurite extension and to control the directional outgrowth of neurites. This micropatterned decellularized ECM architecture has potential as a regenerative microenvironment for nerve repair.

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“…The migratory property of NL2.3-4 cells was evaluated in rat spinal cord (Hasegawa et al, 2005;Hormigo et al, 2001). NL2.3-4 and L2.3 cells labeled with CFDA-SE were transplanted into adult rat normal spinal cord and the migration pattern of the cells was examined after 3 days.…”

Section: Nl23-4 Cells Migrate More Robustly Than L23 In Normal Rat mentioning

confidence: 99%

Activated Notch1 maintains the phenotype of radial glial cells and promotes their adhesion to laminin by upregulating nidogen

Chang

Mohan

et al. 2008

Glia

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Radial glia are neural stem cells that exist only transiently during central nervous system (CNS) development, where they serve as scaffolds for neuronal migration. Their instability makes them difficult to study, and therefore we have isolated stabilized radial glial clones from E14.5 cortical progenitors (e.g., L2.3) after expression of v-myc. Activated Notch1 intracellular region (actNotch1) promotes radial glia in the embryonic mouse forebrain (Gaiano et al., (2000), and when it was introduced into E14.5 cortical progenitors or radial glial clone L2.3, the cells exhibited enhanced radial morphology and increased expression of the radial glial marker BLBP. A representative clone of L2.3 cells expressing actNotch1 called NL2.3-4 migrated more extensively than L2.3 cells in culture and in white matter of the adult rat spinal cord. Microarray and RT-PCR comparisons of mRNAs expressed in these closely related clones showed extensive similarities, but differed significantly for certain mRNAs including several cell adhesion molecules. Cell adhesion assays demonstrated significantly enhanced adhesion to laminin of NL2.3-4 by comparison to L2.3 cells. The laminin binding protein nidogen was the most highly induced adhesion molecule in NL2.3-4, and immunological analyses indicated that radial glia synthesize and secrete nidogen. Adhesion of NL2.3-4 cells to laminin was inhibited by anti-nidogen antibodies and required the nidogen binding region in laminin, indicating that nidogen promotes cell adhesion to laminin. The combined results indicate that persistent expression of activated Notch1 maintains the phenotype of radial glial cells, inhibits their differentiation, and promotes their adhesion and migration on a laminin/nidogen complex.

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Radial Glial Cell Line C6-R Integrates Preferentially in Adult White Matter and Facilitates Migration of Coimplanted Neurons in Vivo

Cited by 14 publications

References 46 publications

Further characterization of embryonic stem cell‐derived radial glial cells

Further characterization of embryonic stem cell‐derived radial glial cells

Nerve Guidance by a Decellularized Fibroblast Extracellular Matrix

Activated Notch1 maintains the phenotype of radial glial cells and promotes their adhesion to laminin by upregulating nidogen

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