RADA is a main branch migration factor in plant mitochondrial recombination and its defect leads to mtDNA instability and cell cycle arrest

Chevigny, Nicolas; Weber-Lotfi, Frédérique; Nadiras, C.; Fertet, Arnaud; Ret, Monique Le; Bichara, Marc; Erhardt, Mathieu; Dietrich, André; Raynaud, Cécile; Guallberto, José

doi:10.1101/856716

Cited by 2 publications

(2 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This pathway could involve RADA, the plant ortholog of eubacterial RadA. In agreement, we found that in Arabidopsis the RADA protein is addressed to mitochondria and chloroplasts, and that the loss of the RADA gene has severe effects on mtDNA stability and plant development [165]. It is also possible that an additional factor evolved to assume branch-migration functions, and RECA3 could be such a factor.…”

Section: Recasupporting

confidence: 80%

DNA Repair and the Stability of the Plant Mitochondrial Genome

Chevigny

Schatz-Daas

Lotfi

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

The mitochondrion stands at the center of cell energy metabolism. It contains its own genome, the mtDNA, that is a relic of its prokaryotic symbiotic ancestor. In plants, the mitochondrial genetic information influences important agronomic traits including fertility, plant vigor, chloroplast function, and cross-compatibility. Plant mtDNA has remarkable characteristics: It is much larger than the mtDNA of other eukaryotes and evolves very rapidly in structure. This is because of recombination activities that generate alternative mtDNA configurations, an important reservoir of genetic diversity that promotes rapid mtDNA evolution. On the other hand, the high incidence of ectopic recombination leads to mtDNA instability and the expression of gene chimeras, with potential deleterious effects. In contrast to the structural plasticity of the genome, in most plant species the mtDNA coding sequences evolve very slowly, even if the organization of the genome is highly variable. Repair mechanisms are probably responsible for such low mutation rates, in particular repair by homologous recombination. Herein we review some of the characteristics of plant organellar genomes and of the repair pathways found in plant mitochondria. We further discuss how homologous recombination is involved in the evolution of the plant mtDNA.

show abstract

Section: Recasupporting

confidence: 80%

DNA Repair and the Stability of the Plant Mitochondrial Genome

Chevigny

Schatz-Daas

Lotfi

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…RuvABC has been shown to play a key role in branch migration during SDSA or SSA repair in bacterial cells. Although, no homolog of bacterial RuvABC is found in plant mitochondria, RadA and RecG proteins have been found to play a similar role in plant mitochondria ( Whitby et al, 1994 ; Marie et al, 2017 ; Chevigny et al, 2019 ). RecG is involved in the migration of the holiday junctions to the very end of the DNA molecule and is also involved in D-loop formation.…”

Section: Dna Damage Repair Mechanisms In Plant Mitochondria and Chloroplastmentioning

confidence: 99%

An Insight Into the Mechanism of Plant Organelle Genome Maintenance and Implications of Organelle Genome in Crop Improvement: An Update

Mahapatra

Banerjee

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Besides the nuclear genome, plants possess two small extra chromosomal genomes in mitochondria and chloroplast, respectively, which contribute a small fraction of the organelles’ proteome. Both mitochondrial and chloroplast DNA have originated endosymbiotically and most of their prokaryotic genes were either lost or transferred to the nuclear genome through endosymbiotic gene transfer during the course of evolution. Due to their immobile nature, plant nuclear and organellar genomes face continuous threat from diverse exogenous agents as well as some reactive by-products or intermediates released from various endogenous metabolic pathways. These factors eventually affect the overall plant growth and development and finally productivity. The detailed mechanism of DNA damage response and repair following accumulation of various forms of DNA lesions, including single and double-strand breaks (SSBs and DSBs) have been well documented for the nuclear genome and now it has been extended to the organelles also. Recently, it has been shown that both mitochondria and chloroplast possess a counterpart of most of the nuclear DNA damage repair pathways and share remarkable similarities with different damage repair proteins present in the nucleus. Among various repair pathways, homologous recombination (HR) is crucial for the repair as well as the evolution of organellar genomes. Along with the repair pathways, various other factors, such as the MSH1 and WHIRLY family proteins, WHY1, WHY2, and WHY3 are also known to be involved in maintaining low mutation rates and structural integrity of mitochondrial and chloroplast genome. SOG1, the central regulator in DNA damage response in plants, has also been found to mediate endoreduplication and cell-cycle progression through chloroplast to nucleus retrograde signaling in response to chloroplast genome instability. Various proteins associated with the maintenance of genome stability are targeted to both nuclear and organellar compartments, establishing communication between organelles as well as organelles and nucleus. Therefore, understanding the mechanism of DNA damage repair and inter compartmental crosstalk mechanism in various sub-cellular organelles following induction of DNA damage and identification of key components of such signaling cascades may eventually be translated into strategies for crop improvement under abiotic and genotoxic stress conditions. This review mainly highlights the current understanding as well as the importance of different aspects of organelle genome maintenance mechanisms in higher plants.

show abstract

RADA is a main branch migration factor in plant mitochondrial recombination and its defect leads to mtDNA instability and cell cycle arrest

Cited by 2 publications

References 77 publications

DNA Repair and the Stability of the Plant Mitochondrial Genome

DNA Repair and the Stability of the Plant Mitochondrial Genome

An Insight Into the Mechanism of Plant Organelle Genome Maintenance and Implications of Organelle Genome in Crop Improvement: An Update

Contact Info

Product

Resources

About