RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis

Wang, Sheng; Wen, Rui; Shi, Xinjie; Lambrecht, Amanda D.; Wang, Hong; Xiao, Wei

doi:10.1016/j.dnarep.2011.04.009

Cited by 30 publications

(26 citation statements)

References 144 publications

(199 reference statements)

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“…b Protection of the ubc13 mutant by OsUBC13 from representative DNA-damaging agents by a serial dilution assay. Cells were grown overnight in SD selective media, diluted and treated as previously described (Wang et al [2011]). Photographs were taken after 2-day incubation at 30°C.…”

Section: Resultsmentioning

confidence: 99%

Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage

Zang

Wang

Xue

et al. 2012

Rice

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While plant growth and reproduction is dependent on sunlight, UV irradiation from sunlight is one of the major genotoxic stresses that threaten plant survival and genome stability. In addition, many environmental chemicals can also damage the plant genome. In yeast and mammalian cells protection against the above genome instability is provided by an error-free DNA-damage tolerance (DDT) pathway, which is dependent on Ubc13-mediated K63-linked polyubiquitination of the proliferating cell nuclear antigen (PCNA). In this study, we isolated the UBC13 gene from rice and characterized its functions. Expression of OsUBC13 can protect a yeast ubc13 null mutant against spontaneous and environmental DNA damage. Furthermore, OsUbc13 physically interacts with human Ubc13 partners Mms2 and Uev1A, and catalyzes K63 polyubiquitination in vitro. These observations collectively suggest that the K63 polyubiquitination is conserved in rice, and that OsUBC13 may be involved in DDT and other cellular processes. In addition, OsUBC13 is constitutively expressed at a high level even under various stress conditions, suggesting that it is a housekeeping gene.Electronic supplementary materialThe online version of this article (doi:10.1186/1939-8433-5-24) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage

Zang

Wang

Xue

et al. 2012

Rice

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“…Currently, the only structural information on the complex concerns the REV7-interacting peptide from human REV3 in complex with REV7 showing how the interaction could produce an interface for the binding of REV1 (Hara et al 2010). Despite the report of an antibody that can detect endogenous REV3 (Wang et al 2011), the size of Pol z, along with its apparently very low levels of expression in cells (Ogawara et al 2010), has significantly hampered biochemical and cell biological studies. Further, unlike its yeast counterpart, deletion of Pol z in mice results in embryonic lethality (Bemark et al 2000;Esposito et al 2000;Wittschieben et al 2000;Kajiwara et al 2001;O-Wang et al 2002;Van Sloun et al 2002) and senescence of primary fibroblasts (Lange et al 2012).…”

Section: Pol Kmentioning

confidence: 99%

Translesion DNA Synthesis and Mutagenesis in Eukaryotes

Sale

2013

Cold Spring Harbor Perspectives in Biology

221

231

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The structural features that enable replicative DNA polymerases to synthesize DNA rapidly and accurately also limit their ability to copy damaged DNA. Direct replication of DNA damage is termed translesion synthesis (TLS), a mechanism conserved from bacteria to mammals and executed by an array of specialized DNA polymerases. This chapter examines how these translesion polymerases replicate damaged DNA and how they are regulated to balance their ability to replicate DNA lesions with the risk of undesirable mutagenesis. It also discusses how TLS is co-opted to increase the diversity of the immunoglobulin gene hypermutation and the contribution it makes to the mutations that sculpt the genome of cancer cells.

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“…K63-linked chains also play a role in the plant response to replication stress. A loss-of-function mutation for the Arabidopsis E3 ligase RAD5 homolog, rad5a, leads to DNA damage sensitivity and has been shown to be important for the error-free DNA repair response (Chen et al, 2008;Mannuss et al, 2010;Wang et al, 2011), suggesting an implication of K63 polyUb chains in postreplication repair in Arabidopsis.…”

Section: K63-linked Ub Chains and Dna Repairmentioning

confidence: 99%

Proteasome‐independent functions of lysine‐63 polyubiquitination in plants

Romero-Barrios

Vert

2017

New Phytologist

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Contents Summary995I.Introduction995II.The plant Ub machinery996III.From Ub to Ub linkage types in plants997IV.Increasing analytical resolution for K63 polyUb in plants998V.How to build K63 polyUb chains?998VI.Cellular roles of K63 polyUb in plants999VII.Physiological roles of K63 polyUb in plants1004VIII.Future perspectives: towards the next level of the Ub code1006Acknowledgements1006References1007 Summary Ubiquitination is a post‐translational modification essential for the regulation of eukaryotic proteins, having an impact on protein fate, function, localization or activity. What originally appeared to be a simple system to regulate protein turnover by the 26S proteasome is now known to be the most intricate regulatory process cells have evolved. Ubiquitin can be arranged in countless chain assemblies, triggering various cellular outcomes. Polyubiquitin chains using lysine‐63 from ubiquitin represent the second most abundant type of ubiquitin modification. Recent studies have exposed their common function in proteasome‐independent functions in non‐plant model organisms. The existence of lysine‐63 polyubiquitination in plants is, however, only just emerging. In this review, we discuss the recent advances on the characterization of ubiquitin chains and the molecular mechanisms driving the formation of lysine‐63‐linked ubiquitin modifications. We provide an overview of the roles associated with lysine‐63 polyubiquitination in plant cells in the light of what is known in non‐plant models. Finally, we review the crucial roles of lysine‐63 polyubiquitin‐dependent processes in plant growth, development and responses to environmental conditions.

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RAD5a and REV3 function in two alternative pathways of DNA-damage tolerance in Arabidopsis

Cited by 30 publications

References 144 publications

Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage

Rice UBC13, a candidate housekeeping gene, is required for K63-linked polyubiquitination and tolerance to DNA damage

Translesion DNA Synthesis and Mutagenesis in Eukaryotes

Proteasome‐independent functions of lysine‐63 polyubiquitination in plants

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