Racing pigeon identification using STR and chromo‐helicase DNA binding gene markers

Lee, James Chun-I; Tsai, Li-Chin; Kuan, Yuan-Yang; Chien, Wen-Hsien; Chang, Kai-Tai; Wu, Cheng‐Hsien; Linacre, Adrian; Hsieh, Hsing-Mei

doi:10.1002/elps.200700063

Cited by 26 publications

(17 citation statements)

References 22 publications

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“…The results showed that DNA not less than 0.000977 ng (a 1 in 1024 dilution) and 0.003906 ng (a 1 in 256 dilution) can be amplified successfully with the primer pairs PG‐L81/PG‐H520 and PGD‐VNTR‐F2/PGD‐VNTR‐R1, respectively. In our previous report using nuclear STR loci in the identification for C. livia , the results showed that all the eight loci were successfully and correctly amplified when the DNA template was not less than 0.0156 ng 1. Our current data reaffirm the phenomenon that identification of the mitochondrial DNA is more sensitive than the nuclear DNA.…”

Section: Resultssupporting

confidence: 83%

“…There has been previous examination of nuclear STR identification for pigeon species ( Columba livia ) 1. Nuclear DNA is suitable for immediate genetic relationship testing but to increase the efficiency of relationship identification and overcome the obstacles of STR identification from highly degraded DNA, it is useful to develop a system for maternal identification by mtDNA.…”

Section: Introductionmentioning

confidence: 99%

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Tetrathiafulvalene Radical Cation Dimerization in a Bistable Tripodal [4]Rotaxane

Aprahamian

Olsen

Trabolsi

et al. 2008

Chemistry A European J

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The template-directed synthesis of a bistable tripodal [4]rotaxane, which has cyclobis(paraquat-p-phenylene) (CBPQT4+) as the pi-electron-deficient rings, and tetrathiafulvalene (TTF) and 1,5-dioxynaphthalene units as the pairs of pi-electron-rich recognition sites located on all three legs of the tripodal dumbbell, is described. The chemical and electrochemical oxidation of the [4]rotaxane and its tripodal dumbbell have allowed us to unravel an unprecedented TTF.+ radical cation dimerization. In fact, two types of TTF dimers, namely, the radical cation dimer [TTF.+]2 and the mixed-valence one [(TTF)2].+, have been observed at room temperature for the tripodal dumbbell, whereas, in the case of the [4]rotaxane, only the radical cation dimer [TTF.+]2 is formed. This anomaly can be explained if it is accepted that most of the neutral TTF units in the [4]rotaxane are encircled by CBPQT4+ rings, which renders the formation of the mixed-valence dimer [(TTF)2].+ highly unfavorable.

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Section: Resultssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Tetrathiafulvalene Radical Cation Dimerization in a Bistable Tripodal [4]Rotaxane

Aprahamian

Olsen

Trabolsi

et al. 2008

Chemistry A European J

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“…For this study, a sexing marker was added based on previously published sequence information (Lee et al . ; Huang et al . ).…”

Section: Methodsmentioning

confidence: 99%

An evaluation of the International Society for Animal Genetics recommended parentage and identification panel for the domestic pigeon (Columba livia domestica)

Groot¹,

Haeringen²

2017

Animal Genetics

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In this study, the International Society for Animal Genetics (ISAG) recommended panel for the identification of the domestic pigeon (Columba livia domestica) is characterized based on commonly used statistical parameters. The marker panel is based on 16 short tandem repeat (STR) loci (PIGN15, PIGN10, PIGN57, PIGN26, CliμD16, CliμD19, PIGN12, CliμD17, CliμT17, PIGN04, CliμD01, CliμD11, CliμD35, CliμT02, CliμT13, CliμT43). The alleles of the 16 loci consist of a mixture of tri-, tetra-, penta- and hexameric repeat patterns. A sex determination marker was included in the multiplex for quality control. The repeat sequence of the PIGN markers was previously unpublished and therefore sequenced to reveal the sequence pattern. In total, 1421 pigeons were genotyped on 16 STR loci to generate allele frequency data for each locus. For all 16 markers combined, a PE1 (combined non-exclusion probability, first parent) of 0.9986 and PE2 (combined non-exclusion probability, second parent) of >0.9999 was observed. Comparing the alleged father and mother, a PE value of >0.9999 was observed. Two of the markers, CliμD19 and PIGN12, were found to have relatively high Hardy-Weinberg equilibrium and F(null) values. Therefore these markers may be considered to be replaced by other STRs. Another point of discussion may be to add a gender identification marker to the recommended ISAG panel. Not only can this serve as an extra identification marker, but this can also confirm the sex of a sample, because it is challenging to determine the sex based on phenotypical characteristics, especially for chicks. In conclusion, the set of 16 STR markers can be used in routine parentage verification and the identification of individuals.

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“…STRs are being developed to individually identify different species [122,[128][129][130][131][132][133][134][135][136] and should only react with the species for which they are designed although some cross reaction has been observed with human STR tests [137][138][139][140][141]. The main roadblock in the development of these tests is that the process of identifying suitable STRs, and the subsequent multiplexing and validation are expensive and time consuming.…”

Section: Future Prospectsmentioning

confidence: 99%

DNA typing in wildlife crime: recent developments in species identification

Tobe

Linacre

2010

Forensic Sci Med Pathol

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Species identification has become a tool in the investigation of acts of alleged wildlife crimes. This review details the steps required in DNA testing in wildlife crime investigations and highlights recent developments where not only can individual species be identified within a mixture of species but multiple species can be identified simultaneously. 'What species is this?' is a question asked frequently in wildlife crime investigations. Depending on the material being examined, DNA analysis may offer the best opportunity to answer this question. Species testing requires the comparison of the DNA type from the unknown sample to DNA types on a database. The areas of DNA tested are on the mitochondria and include predominantly the cytochrome b gene and the cytochrome oxidase I gene. Standard analysis requires the sequencing of part of one of these genes and comparing the sequence to that held on a repository of DNA sequences such as the GenBank database. Much of the DNA sequence of either of these two genes is conserved with only parts being variable. A recent development is to target areas of those sequences that are specific to a species; this can increase the sensitivity of the test with no loss of specificity. The benefit of targeting species specific sequences is that within a mixture of two of more species, the individual species within the mixture can be identified. This identification would not be possible using standard sequencing. These new developments can lead to a greater number of samples being tested in alleged wildlife crimes.

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Racing pigeon identification using STR and chromo‐helicase DNA binding gene markers

Cited by 26 publications

References 22 publications

Tetrathiafulvalene Radical Cation Dimerization in a Bistable Tripodal [4]Rotaxane

Tetrathiafulvalene Radical Cation Dimerization in a Bistable Tripodal [4]Rotaxane

An evaluation of the International Society for Animal Genetics recommended parentage and identification panel for the domestic pigeon (Columba livia domestica)

DNA typing in wildlife crime: recent developments in species identification

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