Racemization of aspartic acid from human dentin in the estimation of chronological age

Yekkala, R.; Meers, Carine; Schepdael, Ann Van; Hoogmartens, Jos; Lambrichts, Ivo; Willems, Guy

doi:10.1016/j.forsciint.2006.02.022

Cited by 51 publications

(42 citation statements)

References 44 publications

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“…Both methods have general used for separation amino acids but gas chromatography has expensive cost and complicate to preparation of sample (Qudsia et al 2014). HPLC method is usually applied in racemization method in recent times (Fu et al 1995;Yekkala et al 2006). There are several advantages of HPLC method such as high sensitivity, shorter analysis time, simple procedure, high reproducibility, lower cost (Benešová et al 2004;Ogino & Ogino 1988;Qudsia et al 2014;Yekkala et al 2006).…”

Section: Analysis Methods For Quantifying D-and L-amino Acidsmentioning

confidence: 99%

“…Aspartic acid (Asp) is non-essential amino acids in the body which commonly used for racemization in age estimation. Racemization of most amino acids is slow process but the accumulation of D-aspartic acids is most rapidly of all stable amino acids in the bones and other tissues (Robins et al 2001;Yekkala et al 2006). Aspartic acid racemization has rate constants at neutral pH (Poinar et al 2006) and can occur throughout lifetime and after death, as well (Yekkala et al 2006).…”

Section: Aspartic Acid Racemizationmentioning

confidence: 99%

“…Racemization of most amino acids is slow process but the accumulation of D-aspartic acids is most rapidly of all stable amino acids in the bones and other tissues (Robins et al 2001;Yekkala et al 2006). Aspartic acid racemization has rate constants at neutral pH (Poinar et al 2006) and can occur throughout lifetime and after death, as well (Yekkala et al 2006). There was a report about racemization rate of many amino acids in fossil and it was found that racemization rate of aspartic acid was highest when compared to other amino acids (Ohtani et al 2002).…”

Section: Aspartic Acid Racemizationmentioning

confidence: 99%

“…This method was briefly as follows: 1) cleaned soft tissues; 2) separate crown and root of teeth by using discs and burs cooled; 3) separated dentin from enamel; 4) coronal dentin was cleaned in distilled water and 0.2M HCL; 5) rinsed with distill water 3 times; 6) used the mortar and pestle to powdered sample; 7) powdered dentin was prepared to hydrolysis step. Another study investigated (Yekkala et al 2006) racemization of aspartic acid from human dentin for age estimation. They collected premolar teeth for analysis.…”

Section: Sample Preparationmentioning

confidence: 99%

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Aspartic Acid Racemization Method for Age Estimation in Human Tissues: A Review

Gumpangseth¹,

Mahakkanukrauh²

2017

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Section: Analysis Methods For Quantifying D-and L-amino Acidsmentioning

confidence: 99%

Section: Aspartic Acid Racemizationmentioning

confidence: 99%

Section: Aspartic Acid Racemizationmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

See 2 more Smart Citations

Aspartic Acid Racemization Method for Age Estimation in Human Tissues: A Review

Gumpangseth¹,

Mahakkanukrauh²

2017

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“…In the living body, AAs that constitute proteins are normally composed of the L-enantiomers, although there are some exceptions that are biologically synthesized using D-enantiomers [3]. With increasing age, a gradual transformation of the L-enantiomers in proteins into D-enantiomers (racemization) occurs; this transformation is influenced by various factors (e.g., temperature, pH) [4]. The racemization also occurs in metabolically inactive tissues such as teeth, eye lenses, vertebral discs, and parts of the brain.…”

Section: Introductionmentioning

confidence: 99%

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

2016

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Abstract:Occurrence of D-amino acids in living organisms is a useful indicator of various changes, diseases, or disorders. Determination of amino acid enantiomers, namely the enantiomeric ratio of amino acids or excess of certain D-amino acids, represents a useful tool in the studies of aging processes or biomarkers in disease/disorder diagnosis in humans. The amount of D-amino acids is usually very low. Therefore, suitable sample pretreatment, often derivatization, and highly selective and sensitive separation methods are essential for D-amino acid analysis in this field. Chromatographic techniques offer appropriate choices for solving these tasks. This review covers the advances in methodology and development of improved instrumental chromatographic methods focused on D,L-amino acid separation and determination. New findings in the area of possible D-amino acid biomarkers are also included.

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Anthropology: Age Determination of Remains

Cattaneo

2009

Wiley Encyclopedia of Forensic Science

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Contrary to sexing, determining the age at death of human remains is much easier when the remains belong to subadults. In this age range, in fact, aging is based on the analysis of the morphological and metric characteristics of bones and teeth and on the stage of development, since each single structure grows and develops at a different rate and in a different manner. Aging, in these cases, is based on the degree of bone growth (e.g., diaphyseal length), epiphyseal fusion, dental eruption, and tooth mineralization. At around 20 years of age (taking into account the influence of ancestry, nutrition, and health status), dental and bone development terminates (apart from areas such as the clavicle). After 25 years of age, one needs to switch to parameters, which rely on degeneration or alteration in time of bony or dental tissues. The most popular methods of age estimation after 25 years use the pubic symphysis, the fourth rib, and the auricular surface of the ilium. Evaluation of cranial sutures is, on the other hand, very variable and less reliable. Degeneration related to age can also be assessed in teeth, where periodontosis and root translucency can provide fairly accurate age estimation. The microscopic study of bone sections may also provide data concerning age through the evaluation of the number of osteons and secondary osteons, of osteon fragments, and of primary osteons by regression formulae. Finally, chemical methods such as aminoacid racemization also have been tested.

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Racemization of aspartic acid from human dentin in the estimation of chronological age

Cited by 51 publications

References 44 publications

Aspartic Acid Racemization Method for Age Estimation in Human Tissues: A Review

Aspartic Acid Racemization Method for Age Estimation in Human Tissues: A Review

Enantiomeric Ratio of Amino Acids as a Tool for Determination of Aging and Disease Diagnostics by Chromatographic Measurement

Anthropology: Age Determination of Remains

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