“…In cells, it blocks serum or platelet-derived growth factor-induced Rac1 activation and lamellipodia formation without affecting the activity of Cdc42 or RhoA (Gao et al, 2004). Therefore, NSC23766 is the most widely used small molecule to inhibit Rac1 activity in different cell types and, most importantly, in in vivo mouse models by injection, intrathecal catheter, or even implanted Alzet osmotic pump (Binker et al, 2008;Müller et al, 2008;Shibata et al, 2008;Shen et al, 2009;Colomba et al, 2011;Tan et al, 2011, Competitive Antagonism of NSC23766 at mAChRs 2012; Ma et al, 2013). From different studies in which NSC23766 was used to inhibit Rac1-dependent responses, IC 50 values of 50-100 mM were reported (Montalvo-Ortiz et al, 2012).…”