Rabs on the fly: Functions of Rab GTPases during development

Caviglia, Sara; Flores-Benítez, David; Lattner, Johanna; Luschnig, Stefan; Brankatschk, Marko

doi:10.1080/21541248.2017.1279725

Cited by 9 publications

(9 citation statements)

References 72 publications

(74 reference statements)

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“…Rab7 is a molecule known to regulate late endosomal traffic and is enriched on lysosomes . In addition, Rab7 has often been found on ER exit sites where it co‐localizes with Rab1 . The association of Rab7 with ER/ cis ‐Golgi membranes may indicate the formation of specialized Rab7 compartments that enter non‐canonical trafficking routes .…”

Section: Resultsmentioning

confidence: 99%

“…Recent findings regarding the principles of intracellular protein/membrane trafficking have shown that different cell types show a surprising versatility with regards to their usage of the intracellular transport machinery. Although the Rab protein family regulates intracellular trafficking steps across various cell classes (ie, epithelia), the composition, organization and trafficking function of Rab proteins often seems different . Therefore, it is vital to chart and understand intracellular trafficking pathways in as many suitable model systems as possible to describe general transport principles, like continuous or pulsed secretion …”

Section: Discussionmentioning

confidence: 99%

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Rab‐mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity

Prince

Kroeger

Gligorov

et al. 2018

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The male seminal fluid contains factors that affect female post‐mating behavior and physiology. In Drosophila, most of these factors are secreted by the two epithelial cell types that make up the male accessory gland: the main and secondary cells. Although secondary cells represent only ~4% of the cells of the accessory gland, their contribution to the male seminal fluid is essential for sustaining the female post‐mating response. To better understand the function of the secondary cells, we investigated their molecular organization, particularly with respect to the intracellular membrane transport machinery. We determined that large vacuole‐like structures found in the secondary cells are trafficking hubs labeled by Rab6, 7, 11 and 19. Furthermore, these organelles require Rab6 for their formation and many are essential in the process of creating the long‐term postmating behavior of females. In order to better serve the intracellular membrane and protein trafficking communities, we have created a searchable, online, open‐access imaging resource to display our complete findings regarding Rab localization in the accessory gland.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Rab‐mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity

Prince

Kroeger

Gligorov

et al. 2018

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“…Ras-related proteins (Rabs) are a family of GTP-binding proteins (GTPases), and are among the most abundant proteins in exosomes 24 . Rabs are molecules necessary for vesicular movement and plasma membrane docking, and play an important role in the formation, transport, adhesion, anchoring and fusion of vesicles 25 . It is considered to be a molecular switch for vesicular transport between organelles, and participates in mediating exosome secretion 26 .…”

Section: Introductionmentioning

confidence: 99%

LncRNA EBLN3P promotes the progression of osteosarcoma through modifying the miR-224-5p/Rab10 signaling axis

Dai

Zhou

et al. 2021

Sci Rep

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The treatment of patients with advanced-stage osteosarcoma represents a major challenge, with very few treatments currently approved. Although accumulating evidence has demonstrated the importance of lncRNAs in osteosarcoma, the current knowledge on the functional roles and molecular mechanisms of lncRNA endogenous born avirus-like nucleoprotein (EBLN3P) is limited. At present, the expressions of EBLN3P and miR-224-5p in osteosarcoma tissues were quantified by reverse transcription-quantitative PCR assay, and the expression of Ras-related protein 10 (Rab10) in osteosarcoma tissues was quantified by immunohistochemistry and western-blotting. The bioinformatics prediction software ENCORI was used to predict the putative binding sites of EBLN3P, Rab10 and miR-224-5p. The regulatory role of EBLN3P or miR-224-5p on cell proliferation, migration and invasion ability were verified by Cell Counting Kit-8, wound healing and Transwell assays, respectively. The interaction among EBLN3P, miR-224-5p and Rab10 were testified by luciferase. The increased expression of EBLN3P and Rab10 and decreased expression of miR-224-5p were observed in osteosarcoma tissues and cell lines. Besides, the overexpression of EBLN3P or knockdown of miR-224-5p were revealed to promote the proliferation, migration and invasion of osteosarcoma cells. Bioinformatics analysis and luciferase assay revealed that EBLN3P could directly interacted with miR-224-5p to attenuate miR-224-5p binding to the Rab10 3′-untranslated region. Furthermore, the mechanistic investigations revealed activation of the miR-224-5p/Rab10 regulatory loop by knockdown of miR‐372-3p or overexpression of Rab10, thereby confirming the in vitro role of EBLN3P in promoting osteosarcoma cell proliferation, migration and invasion. To the best of our knowledge, the present study is the first to demonstrate that EBLN3P may act as a competitive endogenous RNA to modulate Rab10 expression by competitive sponging to miR-224-5p, leading to the regulation of osteosarcoma progression, which indicates a possible new approach to osteosarcoma diagnosis and treatment.

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“…Rab GTPases along with their effectors, coordinate vesicle trafficking functions such as formation of vesicles, fusion of vesicles, directing vesicles to target membranes (reviewed in (Caviglia et al ., 2019)). In wing discs, many Rab proteins are apically enriched.…”

Section: Resultsmentioning

confidence: 99%

Quantitative analysis of three-dimensional cell organisation and concentration profiles within curved epithelial tissues

Prabhakara

Iyer

Rao

et al. 2022

Preprint

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Organogenesis involves folding of flat epithelial tissues into three-dimensional (3D) shapes. Quantitative analysis in 3D of the concentration gradients of biochemical and mechano-chemical cues that shape the tissue has remained a challenge, due to the complex tissue geometries. Towards addressing this, we present a methodology that transforms the cartesian images acquired by high-resolution confocal microscopy of curved tissues into a laminar organisation from the outer-most tissue surface. We further detail a data-based intensity correction method to account for the intensity variations that arise as a consequence of the global geometry. Applying our approach to the dome-shaped Drosophila wing disc, we quantitatively estimate the concentration profiles of different biochemical signals, including the Wingless morphogen gradient. The laminar data-organisation method also enabled visualisation of apical and basal layers facilitating an accurate 3D reconstruction of cell shapes within the pseudostratified epithelium. We find that the columnar disc proper cells have irregular 3D shapes and undergo frequent apico-basal cell intercalations, concomitantly leading to cell neighbour exchanges along the apico-basal axis. The workflow of image processing described here may be employed to quantify 3D concentration gradients and 3D cellular organisation in any layered curved tissue, provided a marker describing the reference surface manifold is available.

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Rabs on the fly: Functions of Rab GTPases during development

Cited by 9 publications

References 72 publications

Rab‐mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity

Rab‐mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity

LncRNA EBLN3P promotes the progression of osteosarcoma through modifying the miR-224-5p/Rab10 signaling axis

Quantitative analysis of three-dimensional cell organisation and concentration profiles within curved epithelial tissues

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