Rabies virus in insectivorous bats: Implications of the diversity of the nucleoprotein and glycoprotein genes for molecular epidemiology

Oliveira, Rafael de Novaes; Souza, Samilly Evangelista; Lobo, Renata Spinelli Vaz; Castilho, Juliana Galera; Macedo, Carla Isabel; Carnieli, Pedro; Fahl, W. O.; Achkar, Samira Maria; Scheffer, K. C.; Kotait, Ivanete; Carrieri, Maria Luíza; Brandão, Paulo Eduardo

doi:10.1016/j.virol.2010.05.030

Cited by 54 publications

(60 citation statements)

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“…This cluster presented two substitutions: one at 367 position from glutamine to lysine and another at 433 position from alanine to serine; these substitutions were also observed by Oliveira et al 25 . However, the amino acid substitution was founded the role in the pathogenesis, and viral entry is unknown and should be the objective of future studies.…”

Section: Conflict Of Interest Financial Support Referencessupporting

confidence: 81%

“…described the substitution of the glutamic acid in the 368 position of the PV sample by aspartic acid in Myotis bats samples 25 as observed in this cluster.…”

Section: Conflict Of Interest Financial Support Referencessupporting

confidence: 56%

“…The genetic lineage identified in the samples from group 3 was previously related 25 to the Myotis bat genus. The same research group The authors declare that there is no conflict of interest.…”

Section: Discussionmentioning

confidence: 78%

“…All samples belonged to the Lyssavirus genus, Rabies virus species. The reconstruction of the phylogenetic tree, with samples from the studied region, demonstrated that all segregated with samples available at GenBank and representative of genetic lineages related to bats species already identified [24][25][26][27] . The observed clusters were supported by high bootstrap values (91% to 100%).…”

Section: Discussionmentioning

confidence: 99%

“…To compare results, only the region until the stop codon of the nucleoprotein gene was considered, as also described by Oliveira et al 25 One sample from an Artibeus lituratus frugivorous bat was segregated with samples related to the genetic lineage maintained by Tadarida brasiliensis bats. This substitution was observed in one tyrosine found in the Pasteur Virus (PV) sample (NC_001542) by asparagine in Brazilian samples 25 ; this substitution was also observed in the studied samples that were segregated in this cluster. This group presented the lowest average intrinsic distance within group.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of rabies virus isolated from non-haematophagous bats in Brazil

Albas

Campos

Araújo

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction:Rabies is an important zoonosis that causes thousands of deaths worldwide each year. Although the terrestrial cycle, mainly transmitted by dogs, is controlled in Brazil, the aerial cycle remains a serious public health issue, besides the economic problem. In the aerial cycle, the haematophagous bat Desmodus rotundus is the main source of infection, where several different species of non-haematophagous bats can be infected and can transmit the virus. Methods: The aim of this work was to study the epidemiological pattern of rabies using antigenic characterization with monoclonal antibodies and genetic characterization by reversetranscriptase polymerase chain reaction followed by sequencing and phylogenetic analysis of non-haematophagous bats' and herbivorous animals' central nervous system samples from the western region of the State of São Paulo, Brazil. Results: From 27 samples, 3 antigenic variants were identified: AgV-3, AgV-4, and AgV-6; and from 29 samples, 5 different clusters were identified, all belonging to the rabies virus species. Conclusions: Although only nonhaematophagous bats were evaluated in the studied region, the majority of samples were from antigenic and genetic variants related to haematophagous bats Desmodus rotundus. Samples from the same antigenic variant were segregated in more than one genetic cluster. This study demonstrated the diversity of rabies virus genetic lineages presented and circulating in nonhaematophagous bats in the studied region.

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confidence: 81%

“…described the substitution of the glutamic acid in the 368 position of the PV sample by aspartic acid in Myotis bats samples 25 as observed in this cluster.…”

Section: Conflict Of Interest Financial Support Referencessupporting

confidence: 56%

Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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