Rabies Virus Expressing Dendritic Cell-Activating Molecules Enhances the Innate and Adaptive Immune Response to Vaccination

Wen, Yiping; Wang, Hualei; Wu, Hua; Fu-he, Yang; Tripp, Ralph A.; Hogan, Robert J.; Fu, Zhen F.

doi:10.1128/jvi.01552-10

Cited by 94 publications

(151 citation statements)

References 55 publications

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“…Wen et al, reported that increased expression of CD11c positive DCs is essential in inducing protective immunity in response to rabies virus [30]. In this study, we have also shown increased expression of peritoneal CD11c + cells in the extract 300ET+Rb immunized group as compared to Rb alone immunized group.…”

Section: Discussionsupporting

confidence: 76%

Supercritical Carbon Dioxide Extract of Seabuckthorn Leaves Enhances Rabies Virus Neutralizing Antibody Titers and CTL Response in Swiss Albino Mice

Jayashankar¹,

Singh²,

Mishra³

et al. 2016

J Vaccines Immunol

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Introduction: Rabies is a viral disease that causes nearly thousands of death globally per year. Vaccination against rabies generates virus neutralizing antibodies and is the most successful and cost effective method of preventing the disease. In the present study, we have evaluated the adjuvant property of supercritical carbon dioxide extract (SCE) 300ET of Seabuckthorn (SBT) leaves against inactivated rabies virus antigen in Swiss albino mice. Methods:Mice were grouped as PBS control; inactivated rabies antigen (Rb) control; 300ET+Rb and Algel+Rb. All the mice were primed on day 1 followed by single booster at day 14. Sera were collected at different time points for RVNA analysis. Additionally, the effect of SCE on CD8 + Granzyme B + Cytotoxic T Lymphocyte (CTL) response, surface markers and cytokine levels were measured. Result:The results showed enhanced rabies virus neutralizing antibody (RVNA) titres using 300ET as compared to control and Algel groups as measured by Rapid Fluorescent Focus Inhibition Test (RFFIT). Moreover, the extract 300ET and Algel showed increased CD138 + (plasma cells) and CD11c + (dendritic cells) cell population as compared to the inactivated rabies antigen immunized mice. The extract 300ET also stimulated cellular immunity by showing heightened CTL response and proinflammatory cytokines level such as TNF-α and IL-1β. Conclusion:Hence, our results suggested that SCE300ET exhibits adjuvant activity by effectively enhancing antibody as well as cell mediated immunity in response to inactivated rabies antigen. Thus, SCE could be considered as a potential adjuvant candidate for rabies vaccines.

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Section: Discussionsupporting

confidence: 76%

Supercritical Carbon Dioxide Extract of Seabuckthorn Leaves Enhances Rabies Virus Neutralizing Antibody Titers and CTL Response in Swiss Albino Mice

Jayashankar¹,

Singh²,

Mishra³

et al. 2016

J Vaccines Immunol

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“…In an effort to develop more efficacious vaccines that induced broader immune responses and protection against heterologous PRRSV strains, live virus-vectored vaccines were made by expressing immune responsespecific molecules [14,15]. Recruitment and activation of dendritic cells (DCs) plays an important role in both innate and adaptive immune responses to viral infections, including vaccination with live attenuated vaccines [14,16].…”

Section: Introductionmentioning

confidence: 99%

“…Recruitment and activation of dendritic cells (DCs) plays an important role in both innate and adaptive immune responses to viral infections, including vaccination with live attenuated vaccines [14,16]. GM-CSF as one of the most important cytokines, has been used to differentiate DCs from monocytes and bone marrow cells [17], as well as to mature and/or activate DCs in vitro and in vivo [14,18,19]. Therefore, GM-CSF has been widely employed as an attractive adjuvant to apply in therapeutic treatment and in vaccine formulations [14,16,[20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

“…GM-CSF as one of the most important cytokines, has been used to differentiate DCs from monocytes and bone marrow cells [17], as well as to mature and/or activate DCs in vitro and in vivo [14,18,19]. Therefore, GM-CSF has been widely employed as an attractive adjuvant to apply in therapeutic treatment and in vaccine formulations [14,16,[20][21][22][23][24][25]. In this study, we constructed a recombinant PRRSV expressing GM-CSF, and provide evidence that this recombinant vaccine effectively activated DCs and regulate the anti-virus microenvironment in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Construction and in vitro evaluation of a recombinant live attenuated PRRSV expressing GM-CSF

Zhou

Jiang

et al. 2014

Virology Journal

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Background: Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be an important problem for the swine industry. Inactivated vaccines and modified-live virus vaccines are widely used in the field; however, the efficacy of these PRRSV vaccines is suboptimal due to poor immunogenicity. Granulocyte-macrophage colony stimulating factor (GM-CSF) has been extensively used as an effective genetic and protein adjuvant to enhance the efficiencies vaccines expressing tumor or pathogen antigens. The purpose of this study was to determine if GM-CSF could increase the efficiency of PRRSV vaccine. Methods: The GM-CSF gene was inserted in the HuN4-F112 vaccine strain by overlap PCR. The expression of GM-CSF by the recombinant virus was confirmed with methods of indirect immunofluorescent assay (IFA) and Western blotting. The stability of recombinant virus was assessed by cDNA sequence and IFA after 20 passages. To detect the biological activity of GM-CSF expressed by the recombinant virus, bone marrow-derived dendritic cells (BMDCs) were isolated and co-cultured with the recombinant virus or parental virus and the surface phenotypes of BMDCs were examined by flow cytometric analysis. The cytokines secreted by BMDCs infected with PRRSV, or treated with LPS, GM-CSF or medium alone were evaluated by ProcartaPlexTM Multiplex Immunoassays and qRT-PCR. Results: A novel modified-live PRRSV vaccine strain expressing GM-CSF (rHuN4-GM-CSF) was successfully constructed and rescued. The GM-CSF protein was stable expressed in recombinant virus-infected cells after 20 passages. Analysis of virus replication kinetics showed that the novel vaccine strain expressing GM-CSF had a similar replication rate as the parental virus. In vitro studies showed that infection of porcine BMDCs with rHuN4-GM-CSF resulted in increased surface expression of MHCI+, MHCII + and CD80/86+ that was dependent on virus expressed GM-CSF. The expression of representative cytokines was significantly up-regulated when BMDCs were incubated with the recombinant GM-CSF expressing virus.

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“…Since this time the approach has been developed by a number of laboratories in Europe and the United States in pursuit of improved understanding of the biology of the virus and the development of attenuated viruses for use as vaccine candidates [6,7]. One popular approach has been to introduce mammalian innate immune response genes into the rabies virus genome, usually between the glycoprotein gene and the L polymerase gene [8][9][10]. The effect of these introductions when tested in a murine model attenuates the virus through overstimulation of the innate immune response, preventing infection of the central nervous system.…”

mentioning

confidence: 99%

Is Vaccination an Option for Treating Rabies?

Johnson¹

2012

J Vaccines Vaccin

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Rabies Virus Expressing Dendritic Cell-Activating Molecules Enhances the Innate and Adaptive Immune Response to Vaccination

Cited by 94 publications

References 55 publications

Supercritical Carbon Dioxide Extract of Seabuckthorn Leaves Enhances Rabies Virus Neutralizing Antibody Titers and CTL Response in Swiss Albino Mice

Supercritical Carbon Dioxide Extract of Seabuckthorn Leaves Enhances Rabies Virus Neutralizing Antibody Titers and CTL Response in Swiss Albino Mice

Construction and in vitro evaluation of a recombinant live attenuated PRRSV expressing GM-CSF

Is Vaccination an Option for Treating Rabies?

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