Rabies neutralizing antibody detection by indirect immunperoxidase serum neutralization assay performed on chicken embryo related cell line

Cardoso, Tereza Cristina; Silva, Luzia Helena Queiroz da; Albas, Avelino; Ferreira, Helena Lage; Perri, Sílvia Helena Venturoli

doi:10.1590/s0074-02762004000500013

Cited by 17 publications

(9 citation statements)

References 17 publications

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“…By the protocol for the management of dog bites (suspected rabies cases), the dog is required to be put under observation for signs of rabies or PEP initiated immediately on the basis of a risk assessment that takes into consideration the local endemicity of rabies and what is known about the dog [20]. In most communities in Ghana dogs stray and it is barely possible to achieve effective quarantine or humane killing and laboratory confirmation as most protocols demand.…”

Section: Discussionmentioning

confidence: 99%

Dog Bites and Rabies in the Eastern Region of Ghana in 2013–2015: A Call for a One-Health Approach

Adomako

Baiden

Sackey

et al. 2018

Journal of Tropical Medicine

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Background A One-Health approach is advocated to ensure effective rabies surveillance in sub-Saharan Africa. Information is needed to assess the current state of dog bites and rabies in Ghana. We analyzed data on reported events in the Eastern Region of Ghana from 2013 to 2015 to generate information that can be used for rabies elimination in Ghana through the One-Health approach. Method We extracted data on dog bites and rabies from the database of the regional health service and performed descriptive analysis using Epi Info version 7™. We followed up with interviews with three key informants from the health and veterinary services on issues related to surveillance and data quality. Results Overall, 4821 dog bites were reported over the three-year period. This translated into an annual incidence of 172 cases per a population of 100,000. Most of cases were in children aged less than 10 yrs. Fifteen (53.3% males) cases of rabies were recorded in seven out of the 26 municipalities and districts, translating into a rabies to dog bite ratio of 3: 1000. The median age of victims was 9 years (range: 3-72 years). A parallel and uncoordinated system of rabies surveillance is maintained by the health and veterinary services, with gross disparities in the number of reported events and overall impression of underreporting. Conclusion Rabies remains an important cause of preventable deaths in this region. An integrated approach to surveillance based on the One-Health concept needs to be adopted.

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Section: Discussionmentioning

confidence: 99%

Dog Bites and Rabies in the Eastern Region of Ghana in 2013–2015: A Call for a One-Health Approach

Adomako

Baiden

Sackey

et al. 2018

Journal of Tropical Medicine

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“…Two previous studies have reported the utility of an indirect immunoperoxidase test using polyclonal anti-rabies antisera for the detection of RVNA in serum samples. 10,11 In the present study, the sensitivity and specificity of the test were enhanced further with the use of a biotinylated anti-N monoclonal antibody cocktail, which was previously shown to be 100% specific and sensitive when employed in detection of rabies virus N antigen in brain smears in our laboratory.…”

Section: Discussionmentioning

confidence: 77%

Development and evaluation of a new immunohistochemistry-based test for the detection of rabies virus neutralizing antibodies

Madhusudana

Malavalli

Thankappan

et al. 2014

Human Vaccines & Immunotherapeutics

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“…Sugiyama et al. (1997) and Cardoso et al. (2004) reported that the assay correlates the competitive ELISA ( r = 0.90, n = 88) and the mouse neutralization test ( r = 0.99, n = 100 and r = 0.93, n = 99) respectively.…”

Section: Resultsmentioning

confidence: 99%

Validation and Standardization of Virus Neutralizing Test Using Indirect Immunoperoxidase Technique for the Quantification of Antibodies to Rabies Virus

Ogawa

Gamoh

Aoki

et al. 2008

Zoonoses and Public Health

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A virus neutralizing test using an indirect immunoperoxidase technique (VNT-IIP) for rabies has been developed for the titration of dog and cat serum samples in Japan. The VNT-IIP has the advantage that results obtained can be viewed by the naked eye. The purpose of this study was to validate the VNT-IIP and compare it with one of the international standard methods, the fluorescent antibody virus neutralization test (FAVNT). The VNT-IIP showed satisfactory repeatability, high analytical specificity and good accuracy. Regarding the comparison between the VNT-IIP and the FAVNT, the VNT-IIP showed good agreement (91.9%), high sensitivity (92.8%) as well as specificity (87.0%) and good correlation (r = 0.92). As described above, the validation of the VNT-IIP was satisfactory and the performances of the test proved to be equivalent to those of an international standard method.

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Rabies neutralizing antibody detection by indirect immunperoxidase serum neutralization assay performed on chicken embryo related cell line

Abstract: The aim of this study was to evaluate the indirect immunoperoxidase virus neutralization (IPVN)

Cited by 17 publications

References 17 publications

Dog Bites and Rabies in the Eastern Region of Ghana in 2013–2015: A Call for a One-Health Approach

Dog Bites and Rabies in the Eastern Region of Ghana in 2013–2015: A Call for a One-Health Approach

Development and evaluation of a new immunohistochemistry-based test for the detection of rabies virus neutralizing antibodies

Validation and Standardization of Virus Neutralizing Test Using Indirect Immunoperoxidase Technique for the Quantification of Antibodies to Rabies Virus

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