Rabies Diagnosis by Fluorescent Antibody. I—Its Evaluation in a Public Health Laboratory

McQueen, James L.; Lewis, Arthur L.; Schneider, Nathan J.

doi:10.2105/ajph.50.11.1743

Cited by 40 publications

(14 citation statements)

References 3 publications

(1 reference statement)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…10 was conducted by the Division of Consolidated Laboratory Services (Richmond Virginia). The result on fresh brain tissue was negative, excluding rabies as the cause of the central nervous system signs.…”

mentioning

confidence: 99%

Listeriosis Outbreak in Dairy Cattle Caused by an UnusualListeria MonocytogenesSerotype 4b Strain

Bundrant

Hutchins²,

Bakker

et al. 2011

J VET Diagn Invest

View full text Add to dashboard Cite

Abstract. A listeriosis outbreak, in dairy cattle, with a high case mortality and acute death after onset of symptoms was investigated using gross pathology and bacteriologic approaches, including molecular characterization of a clinical Listeria monocytogenes isolate. In a herd of 315 animals, 9 animals showed clinical symptoms consistent with listeriosis, including 3 animals that died within 2-4 days after acute onset of clinical signs, 4 animals that were euthanized, and 2 that survived. Initial EcoRI ribotyping and serotyping indicated that this outbreak was caused by an unusual L. monocytogenes serotype 4b strain, which was classified into lineage III. Further characterization of this isolate by DNA sequencing-based subtyping methods indicated that the strain responsible for this outbreak represented a unique genotype as supported by its classification into a new sigB allelic type, which has not been identified previously among .290 isolates, and by compelling phylogenetic evidence. While lineage III isolates are generally rare, they seem to be more common among L. monocytogenes isolates from animals with clinical signs of listeriosis. This is the first report of a particularly severe clinical course of disease associated with infection by a lineage III strain. The high prevalence of Listeria spp., including L. monocytogenes, in the farm environments may favor emergence and evolution of novel, and possibly more virulent, L. monocytogenes strains. Continued monitoring of animal listeriosis cases and outbreaks may not only improve animal health but also aid in the early discovery of newly emerging L. monocytogenes strains.

show abstract

mentioning

confidence: 99%

Listeriosis Outbreak in Dairy Cattle Caused by an UnusualListeria MonocytogenesSerotype 4b Strain

Bundrant

Hutchins²,

Bakker

et al. 2011

J VET Diagn Invest

View full text Add to dashboard Cite

show abstract

“…Las técnicas utilizadas fueron las de inmunofluorescencia directa sobre material encefálico fresco (16), en la cual anticuerpos policlonales antirrábicos marcados con fluoresceína reconocen antígeno viral presente en dicho material y se produce señal visible al microscopio de fluorescencia. Adicionalmente se emplearon las técnicas de histopatología convencional e inmunohistoquímica utilizando avidina-biotina conjugada con peroxidasa sobre material encefálico en fijado en formol neutro al 10%; el anticuerpo primario policlonal se empleó con dilución 1:160 (17).…”

Section: Diagnostico Y Tipificación Antigénica Del Virus De La Rabiaunclassified

Brotes de rabia humana transmitida por vampiros en los municipios de Bajo y Alto Baudó, departamento del Chocó, Colombia 2004-2005

et al. 2006

View full text Add to dashboard Cite

“…El diagnóstico de la rabia por inmunofluoresçencia (IF) puede ser muy eficiente 1,6,8,11, 12 siempre que se tomen ciertas precauciones, tales como un müestreo adecuado de los especímenes de tejido nervioso 6 , la alineación correcta del microscopio 9 y la estandarización apropiada del conjugado para IF 10 . Esta estandarización es esencial para la eficiencia del método.…”

Section: Introductionunclassified

Evaluacion de un conjugado antirrabico por titulacion en diferentes microscopios fluorescentes

Larghi¹,

Oliva-Pinheiro

Gonzalez-Luarca

1986

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

EVALUACION DE UN CONJUGADO ANTIRRABICO POR TITULACION EN DIFERENTES MICROSCOPIOS FLUORESCENTESO. P. LARGHI (1), O. OLIVA-PINHEIRO (3) y E. GONZALEZ-LUARCA (3) RESUMENEn cinco laboratorios se tituló una partida de conjugado antirrábico para inmunofluoresCencia con microscopios de tres marcas distintas, equipados con diferentes accesorios. Los títulos obtenidos variaron entre < 1:4 y 1:64, dependiendo de los accesorios utilizados. Estos resultados ponen de relieve la necesidad de titular los conjugados en cada laboratorio. Se mencionam los inconvenientes que pueden ocasionar la distribución del reactivo diluido de acuerdo con el título obtenido por el productor, o mezclado con suspensiones de cerebro de ratones normales e infectados con virus rábico.UNITERMOS: Raiva -Imunofluorescência -Sorologia -Teste de qualidade diagnóstica.

show abstract

Rabies Diagnosis by Fluorescent Antibody. I—Its Evaluation in a Public Health Laboratory

Cited by 40 publications

References 3 publications

Listeriosis Outbreak in Dairy Cattle Caused by an UnusualListeria MonocytogenesSerotype 4b Strain

Listeriosis Outbreak in Dairy Cattle Caused by an UnusualListeria MonocytogenesSerotype 4b Strain

Brotes de rabia humana transmitida por vampiros en los municipios de Bajo y Alto Baudó, departamento del Chocó, Colombia 2004-2005

Evaluacion de un conjugado antirrabico por titulacion en diferentes microscopios fluorescentes

Contact Info

Product

Resources

About