Rabbits possess conventional T lymphocytes

Bell, Clara; Wigzell, Hans

doi:10.1002/eji.1830071015

Cited by 21 publications

(7 citation statements)

References 22 publications

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“…Our data are compatible with those of others, who showed, using fluorescence microscopy, that rabbit B and T cells are distinct subpopulations [13,15,[27][28][29][30].…”

Section: Discussionsupporting

confidence: 96%

“…PBL and spleen cells from normal or immunized rabbits behaved functionally as conventional B and T cells since the Ig-TC enriched cells responded well to T cell mitogens but poorly to anti-Ig reagents, whereas the opposite was true for the Ig' cells. These data are fully compatible with those of others who analyzed functional properties of separated lymphocyte populations from various rabbit lymphoid organs [ 13,[27][28][29][30]. In striking contrast to the data on PBL and spleen cells were those obtained with activated LN.…”

Section: Discussionsupporting

confidence: 91%

“…48 h later, [3H]thymidine incorporation was measured. Reagents added were: medium (l), anti-b4 (5186) IgG (2), anti-b4 (5186) Fab (3), anti-b4 (5253 IX) IgG (4), anti-b9 (5242) IgG (5), anti-b5 (5128) IgG (6), anti-a1 (849) IgG(7), anti-a1 (849) Fab(8), anti-a1 (5159) IgG (9), anti-a2 (5223) IgG (lo), anti-a3 (839) IgG ( l l ) , anti-a3 (874) IgG (12), anti-a3 (874) Fab(13), SaRIg(14), GaRFab (15), PHA(16).…”

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confidence: 99%

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Effects of anti‐Ig reagents on T cell functions I. Activation of rabbit T cells by anti‐allotype antibodies

et al. 1982

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Rabbit lymphocytes were analyzed by flow microfluorometry, using anti-T cell and anti-Ig reagents. Rabbit T cells and cells expressing surface Ig (B cells) appeared to belong to distinct subpopulations which could be separated on the basis of their selective adherence to nylon wool columns or to anti-Ig-coated dishes. Using flow microfluorometry, no evidence was obtained for the expression of a allotypes (VH framework) on T cells. Separated lymphocyte populations were functionally characterized using an in vitro proliferation assay. B and T cells from rabbit spleen or peripheral blood responded in a differential fashion to B and T cell-specific mitogens and to anti-Ig antibodies. Although such T cells did not respond upon stimulation with anti-Ig antibodies alone, significant proliferation could be induced by simultaneous addition of anti-Ig and T cell growth factor. In addition, activated T cells, derived from lymph nodes of immunized rabbits, generated a proliferative response upon stimulation with anti-Ig reagents alone. The above-mentioned effects on T cells could be obtained using heterologous anti-Ig antibodies or isologous anti-allotype antibodies, directed either against a allotypes (VH framework) or against b allotypes (kappa light chain). Antibodies against the Fc portion of rabbit Ig or against irrelevant allotypic specificities were ineffective in triggering T cells. Fab fragments from anti-allotype antibodies were equally stimulatory for T cells as compared to intact IgG, indicating that cross-linking of Ig-like molecules is not a necessary requirement for anti-Ig-induced T cell activation.

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“…Our data are compatible with those of others, who showed, using fluorescence microscopy, that rabbit B and T cells are distinct subpopulations [13,15,[27][28][29][30].…”

Section: Discussionsupporting

confidence: 96%

Section: Discussionsupporting

confidence: 91%

mentioning

confidence: 99%

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Effects of anti‐Ig reagents on T cell functions I. Activation of rabbit T cells by anti‐allotype antibodies

et al. 1982

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“…For these cells, published B cell values have varied from over 80°/o, reported by Jones et al [10], to about 70%, reported by Linthicum and Sell [13] and by Bell and Wigzell [1] and, final ly, to low values of about 40%, reported by Fujiwara et al [9] and confirmed by Cavaillon et al [4], This wide range of report ed values may have been caused by differ ences in techniques employed by different investigators. The main factors which may increase the apparent percentage of B cells in a given population are: (1) Formations of aggregates between autologous erythrocytes and T cells during the preparations of peri pheral blood lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

Surface Immunoglobulin Receptors of Rabbit Lymphoid Cells

Szymańska¹,

Alcala²,

Dubiski³

et al. 1980

Int Arch Allergy Immunol

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Rabbit lymphoid cells were stained with fluorescein-labelled antiallotype antibodies. The double layer technique was found to be more sensitive than the direct staining. Rabbit B cells are stained only via their surface immunoglobulin (sIg) receptors and not via the receptors for the Fc portion of the IgG. Peritoneal exudate macrophages do not carry sIg receptors and are stained via their Fc receptors. Removal of protein aggregates from the system and use of reagents prepared from F(ab’)₂ immunoglobulin fragments prevent staining of the, macrophages through their Fc receptors. There was a good agreement between the percent of RABELA-positive and sIg-containing spleen cells. In appendix there were approximately 20% more RABELA-positive than sIg-positive cells.

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“…One set of goat antibodies specific for each human VH subgroup has been prepared by selective adsorption and was found to be useful in typing secreted and membranebound Ig on B cells (3).Evidence from studies (4-8) using anti-idiotype antibodies has suggested that T cells and their antigen-specific, soluble factors might possess Ig-like idiotypic determinants. VH allotypic determinants have also been demonstrable on rabbit T cells by several groups (9, 10) and not by others (11)(12)(13)(14)(15). Antibodies against VH framework regions of the mouse myeloma protein MOPC-315, which cross-react with other mouse Ig regardless of their antibody specificity, class, subclass, subgroup, or allotype (16), may inhibit T cell function (17)(18)(19) and cross-react with soluble factors ofT cells (20,21).…”

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confidence: 99%

Immunoglobulin VH determinants defined by monoclonal antibodies.

Kubagawa¹,

Mayumi²,

Kearney³

et al. 1982

The Journal of Experimental Medicine

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The heavy and light chains of immunoglobulins (Ig) consist of two distinct regions: variable and constant. Variable regions, which determine the antibody specificity and idiotypic determinants, are further divided into three hypervariable regions and four relatively invariant, so-called framework, regions (1, 2). The variable regions of both heavy chains (VH) 1 and light chains (VL) have been divided into subgroups based upon similarities between amino acid sequences in their framework regions. For human Ig, three major VH subgroups have been characterized on the basis of amino acid sequences of the aminoterminal 20 residues within the first framework region (2). One set of goat antibodies specific for each human VH subgroup has been prepared by selective adsorption and was found to be useful in typing secreted and membranebound Ig on B cells (3).Evidence from studies (4-8) using anti-idiotype antibodies has suggested that T cells and their antigen-specific, soluble factors might possess Ig-like idiotypic determinants. VH allotypic determinants have also been demonstrable on rabbit T cells by several groups (9, 10) and not by others (11-15). Antibodies against VH framework regions of the mouse myeloma protein MOPC-315, which cross-react with other mouse Ig regardless of their antibody specificity, class, subclass, subgroup, or allotype (16), may inhibit T cell function (17-19) and cross-react with soluble factors ofT cells (20,21). The molecular nature of antigen receptors on T lymphocytes, however, is still controversial. Recent studies (22, 23) using recombinant DNA techniques have suggested that antigen-specific helper or killer T cell clones may not use the Ig-joining and constant gene segments.We prepared monoclonal hybridoma antibodies against VH fragments isolated from human IgM myeloma proteins with the hope of using these to analyze the products of normal or abnormal B and T cells. This paper describes the preparation and characterization of four such monoclonal anti-human VH antibodies. Our studies *

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Rabbits possess conventional T lymphocytes

Cited by 21 publications

References 22 publications

Effects of anti‐Ig reagents on T cell functions I. Activation of rabbit T cells by anti‐allotype antibodies

Effects of anti‐Ig reagents on T cell functions I. Activation of rabbit T cells by anti‐allotype antibodies

Surface Immunoglobulin Receptors of Rabbit Lymphoid Cells

Immunoglobulin VH determinants defined by monoclonal antibodies.

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