Rabbit sperm cryopreservation: A review

Mocé, E.; Vicente, J.S.

doi:10.1016/j.anireprosci.2008.08.015

Cited by 95 publications

(102 citation statements)

References 74 publications

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“…Non-penetrating cryoprotectants are generally large molecules such as polymers, sugars, proteins or amino acids. Among the disaccharides used as non-permeable CPAs, sucrose has shown a better cryoprotective effect (Mocé and Vicente, 2009). In previous studies (Iaffaldano et al, 2012;Rosato and Iaffaldano, 2013), we developed an effective freezing protocol for rabbit semen based on the use of DMSO in the freezing extender as the permeable CPA in combination with sucrose.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

Iaffaldano¹,

Iorio²,

Rosato³

et al. 2014

Animal Reproduction Science

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Section: Introductionmentioning

confidence: 99%

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

Iaffaldano¹,

Iorio²,

Rosato³

et al. 2014

Animal Reproduction Science

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“…The frozen rabbit sperm has only been used for experimental or genetic resource bank purposes (Mocé and Vicente, 2009). It would be highly beneficial in routine artificial insemination (AI) programmes on commercial rabbit farms if semen could be cryopreserved without affecting fertility, although a multitude of factors affect the effectiveness of cryopreservation, including the initial quality of the semen samples, cryopreservation protocol, freezing extenders and environmental factors (Mocé and Vicente, 2009;Iaffaldano et al, 2012;Lavara et al 2013;Rosato and Iaffaldano, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…It would be highly beneficial in routine artificial insemination (AI) programmes on commercial rabbit farms if semen could be cryopreserved without affecting fertility, although a multitude of factors affect the effectiveness of cryopreservation, including the initial quality of the semen samples, cryopreservation protocol, freezing extenders and environmental factors (Mocé and Vicente, 2009;Iaffaldano et al, 2012;Lavara et al 2013;Rosato and Iaffaldano, 2013). In addition, differences in reproductive parameters have been observed with fresh (Bolet et al, 2000;Vicente et al, 2000) and cryopreserved sperm belonging to different breeds or lines of rabbits.…”

Section: Introductionmentioning

confidence: 99%

“…In this context, the basic ingredients of current sperm-freezing extenders in rabbits are a combination of permeating cryoprotectants such as acetamide or dimethyl sulphoxide (Me 2 SO) and non-permeating cryoprotective agents such as lactose, sucrose, raffinose or trehalose. In addition, egg yolk is often included as an additional protective additive in rabbit sperm-freezing extenders (Fox, 1961;Stranzinger et al, 1971;Mocé and Vicente, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Viudes-de-Castro

Lavara

Safaa

et al. 2014

Animal

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This study was conducted to elucidate the effect of different freezing extenders on two lines selected for hyperprolificacy and longevity (H and LP, respectively). In extender A, dimethyl sulphoxide (Me 2 SO) and sucrose were used as cryoprotectants. In extenders B and C, the sucrose was replaced by 20% egg yolk, and in extender C the Me 2 SO was substituted by acetamide. Semen was packaged in 0.25 ml plastic straws and cooled at 5°C for 45 min, and then was frozen in liquid nitrogen vapour for 10 min before being plunged into the liquid nitrogen. Thawing was carried out by immersing the straws in a water bath at 50°C for 10 s. Frozen-thawed semen characteristics and reproductive parameters were affected by freezing. Extender C showed significantly lower post-thawing quality traits than any of the three extenders. Acrosome integrity was significantly improved when Me 2 SO was used as cryoprotectant. Sucrose replacement by 20% egg yolk had no effect on acrosome integrity but provided significantly lower sperm motility and viability. Freezing extender affected fertility rate, total born, number of implantation sites and gestational losses, obtaining better results when extender A was used. The acrosomal integrity after frozen-thawed process showed a significant correlation with fertility at 12 th day and also at birth, indicating that an increase in acrosomal integrity leads to an increase in both fertilities (12 th day and at birth). A positive correlation between motility of semen and implantation sites was found. The post-thawing quality traits of semen were not affected by the genetic line, although LP line showed higher total born and lower foetal and gestational losses. The findings of this study suggest that freezing extender composition has a significant effect on the success of rabbit sperm for preservation, and when Me 2 SO was used as permeable cryoprotectant sucrose provided better protection compared with egg yolk and improved reproductive traits, and, on the other hand, the male genotypes used in the present study had no effect on frozen-thawed sperm parameters but negatively affected some of the reproductive parameters.

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“…Perhaps, in the future, it may be necessary to use frozen semen, as it is routinely used in other domestic animals for bio-security reasons. For the moment, few studies have been performed on this topic (for review: Mocé and Vicente, 2009), and only Lavara et al (2009) reported h 2 and r estimates of sperm traits after the freezing-thawing process, studied in 315 bucks of R line. After the freezing-thawing process, sperm traits showed low to medium h 2 (0.06, 0.07 and 0.21 for individual sperm motility, normal acrosome status and viability) and r (0.19, 0.14 and 0.46 for individual sperm motility, normal acrosome status and viability).…”

Section: Genetic Variation Within Linesmentioning

confidence: 99%

Breeding programmes to improve male reproductive performance and efficiency of insemination dose production in paternal lines: feasibility and limitations

et al. 2013

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This paper reviews the current genetic knowledge on issues related with the efficient use of bucks in artificial insemination (AI). Differences between lines have been found to be relevant in semen production and quality traits not necessarily related to their specialisation as maternal or paternal lines. Accurate heritability estimates indicate that genetic selection to increase semen production by improving male libido and/or reducing the number of rejected ejaculates may not be effective. However, total sperm produced per ejaculate appears to be an interesting trait to select for. Semen pH has shown low to medium heritability estimates and a low coefficient of variation, therefore it is not advisable to attempt improvement by direct selection. In general, sperm motility traits have shown low heritabilities, but the rate of motile sperms per ejaculate has been considered convenient to select for. Morphological characteristics of the spermatozoa have been revealed as medium to highly heritable. There is evidence of high genetic correlations between sperm traits before and after freezing-thawing. There are few studies regarding the estimation of heterosis of seminal traits, but results indicate important and favourable direct and maternal heterosis in crosses between maternal lines. However, this has not been confirmed in a cross between 2 paternal lines. Until now, attempts to find parametric or non-parametric functions to predict ejaculate fertility through seminal characteristics routinely recorded in evaluations have been very unsatisfactory. Hence, it may be necessary to find other semen quality markers or assess some of those currently used in a more precise manner, or closer to the AI time, in order to improve the ability to predict ejaculate fertility. Several seminal characteristics phenotypically correlated to male fertility could be considered as potential traits to select for in order to genetically improve this trait. However, only the semen pH has been checked for this purpose, and results show a low genetic correlation of this trait with male fertility. Other traits can be studied in the future, but bearing in mind that the required experiments will need a large number of bucks for an accurate estimation of the genetic correlation of the trait with male fertility. This means that these experiments will be expensive and difficult to set up. The most common criterion for selecting paternal lines, average daily gain, seems not to be genetically correlated to male fertility and seminal traits. Thus, selection for average daily gain has no detrimental consequences on these traits, and a multi-trait selection, including growth rate and seminal traits directly related to an efficient AI semen dose production, is feasible in paternal lines. The male contribution to fertility after natural mating and after AI with semen doses with high concentration is negligible, although it has been found that under more restrictive AI conditions male contributions to fertility and litter size are low, but high...

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Rabbit sperm cryopreservation: A review

Cited by 95 publications

References 74 publications

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

Cryopreservation of rabbit semen using non-permeable cryoprotectants: Effectiveness of different concentrations of low-density lipoproteins (LDL) from egg yolk versus egg yolk or sucrose

Effect of freezing extender composition and male line on semen traits and reproductive performance in rabbits

Breeding programmes to improve male reproductive performance and efficiency of insemination dose production in paternal lines: feasibility and limitations

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