Rabbit retinal ganglion cell survival after optic nerve section and its effect on the inner plexiform layer

Germain, Francisco; Calvo, Miguel A.; Villa, Pedro de la

doi:10.1016/j.exer.2003.09.012

Cited by 19 publications

(18 citation statements)

References 29 publications

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“…The second aim of this study was to analyse whether, in spite of cell membrane changes showed by RGCs after ONS, surviving cells were able to maintain electrical responses. Non‐invasive methods (ERG) were used to allow a temporal evaluation.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, Wnt activation, alone or in combination with growth factors, may increase the threshold for apoptosis and halt or delay further retinal degeneration. 43 The second aim of this study was to analyse whether, in spite of cell membrane changes showed by RGCs after ONS, 6,46 surviving cells were able to maintain electrical responses. Non-invasive methods (ERG) were used to allow a temporal evaluation.…”

Section: Discussionmentioning

confidence: 99%

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Electroretinographical and histological study of mouse retina after optic nerve section: a comparison between wild‐type and retinal degeneration 1 mice

Germain

Istillarte

Gómez‐Vicente

et al. 2013

Clinical Exper Ophthalmology

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Background Retinal ganglion cell death underlies the pathophysiology of neurodegenerative disorders such as glaucoma or optic nerve trauma. To assess the potential influence of photoreceptor degeneration on retinal ganglion cell survival, and to evaluate functionality, we took advantage of the optic nerve section mouse model. Methods Surviving retinal ganglion cells were double‐stained by exposing both superior colliculi to fluorogold, and by applying dextran‐tetramethylrhodamine to the injured optic nerve stump. To assess retinal function in wild‐type animals, electroretinograms were recorded on the injured eyes and compared with the contralateral. Similar labelling experiments were carried out on retinal degeneration 1 mice. Surviving retinal ganglion cells were counted 21 days after axotomy and compared with wild‐type mice. No functional experiments were performed on retinal degeneration 1 animals because they do not develop normal electroretinographical responses. Results A significant decrease in retinal ganglion cell density was observed 6 days after axotomy in the wild type. Functional studies revealed that, in scotopic conditions, axotomy induced a significant amplitude decrease in the positive scotopic threshold response component of the electroretinogram. Such decrease paralleled cell loss, suggesting it may be an appropriate technique to evaluate functionality. When comparing retinal ganglion cell densities in wild‐type and retinal degeneration 1 mice, a significant greater survival was observed on the latter. Conclusions After optic nerve section, electroretinographical recordings exhibited a progressive decrease in the amplitude of the positive scotopic threshold response wave, reflecting ganglion cell loss. Interestingly, rod degeneration seemed, at least initially, to protect from axotomy‐driven damage.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Electroretinographical and histological study of mouse retina after optic nerve section: a comparison between wild‐type and retinal degeneration 1 mice

Germain

Istillarte

Gómez‐Vicente

et al. 2013

Clinical Exper Ophthalmology

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“…In nine of the 12 studies that evaluated RGC survival at the above-mentioned time points, an intraorbital injury was performed,38 – 40 42 – 46 48 in two studies, both an intraorbital and intracranial injury was performed,37 47 and in one study, only an intracranial injury was performed 41. The injuries were divided into two categories—intraorbital injury (11 studies) and intracranial injury (three studies).…”

Section: Resultsmentioning

confidence: 99%

“…The results of the studies using intraorbital injuries were as follows: eight studies evaluated RGC survival at the 1–3 day time point with survival ranging from 72 to 100%;37 40 42 – 46 48 11 studies evaluated RGC survival at the 10–15 day time point with results ranging from 5.8 to 27%;37 – 40 42 – 48 and six studies evaluated RGC survival at the 26–30 day time point with results ranging from “negligible” to 7.5% 37 – 40 46 47…”

Section: Resultsmentioning

confidence: 99%

Impediments to eye transplantation: ocular viability following optic-nerve transection or enucleation

Ellenberg

Shi

Jain

et al. 2009

British Journal of Ophthalmology

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Maintenance of ocular viability is one of the major impediments to successful whole-eye transplantation. This review provides a comprehensive understanding of the current literature to help guide future studies in order to overcome this hurdle. A systematic multistage review of published literature was performed. Three specific questions were addressed: (1) Is recovery of visual function following eye transplantation greater in cold-blooded vertebrates when compared with mammals? (2) Is outer retina function following enucleation and reperfusion improved compared with enucleation alone? (3) Following optic-nerve transection, is there a correlation between retinal ganglion cell (RGC) survival and either time after transection or proximity of the transection to the globe? In a majority of the studies performed in the literature, recovery of visual function can occur after whole-eye transplantation in cold-blooded vertebrates. Following enucleation (and reperfusion), outer retinal function is maintained from 4 to 9 h. RGC survival following optic-nerve transection is inversely related to both the time since transection and the proximity of transection to the globe. Lastly, neurotrophins can increase RGC survival following optic-nerve transection. This review of the literature suggests that the use of a donor eye is feasible for whole-eye transplantation.

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“…The degree of I/R-induced retinal neuronal death was quantified by the thickness of the retinal layers at 2 mm from the optic disc under a magnification of 400× with an ocular micrometer. The retinal layers were defined and measured for thickness in accordance with a previous classification TUNEL assay 20 18 The inner layer was defined as the combined ganglion cell layer (GCL) and inner plexiform layer (IPL), while the middle layer included the inner nuclear layer (INL) and the outer plexiform layer (OPL). The thickness of the outer layer only included the outer nuclear layer (ONL).…”

Section: Morphometric Analysismentioning

confidence: 99%

Neuroprotective Effect of Small Interfering RNA Targeted to Caspase-3 on Rat Retinal Ganglion Cell Loss Induced by Ischemia and Reperfusion Injury

Ishikawa

Hirata

Nakabayashi

et al. 2012

Current Eye Research

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Rabbit retinal ganglion cell survival after optic nerve section and its effect on the inner plexiform layer

Cited by 19 publications

References 29 publications

Electroretinographical and histological study of mouse retina after optic nerve section: a comparison between wild‐type and retinal degeneration 1 mice

Electroretinographical and histological study of mouse retina after optic nerve section: a comparison between wild‐type and retinal degeneration 1 mice

Impediments to eye transplantation: ocular viability following optic-nerve transection or enucleation

Neuroprotective Effect of Small Interfering RNA Targeted to Caspase-3 on Rat Retinal Ganglion Cell Loss Induced by Ischemia and Reperfusion Injury

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