Rabbit globulin and antiglobulin factors associated with Treponema pallidum growth in rabbits.

Logan, Leslie C.

doi:10.1136/sti.50.6.421

Cited by 13 publications

(13 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Normal rabbit serum gave some level of protection against a challenge with T. pallidum (Table 3), and consequently all comparisons were made between transfer with immune and normal serum rather than with no serum. The presence of rabbit protein on the surface of the introduced T. pallidum (Logan 1974) may have resulted in a cross-reaction to allotypic differences in these proteins owing to the immunisation of the recipient rabbit with the transferred immune rabbit serum proteins nine days before challenge.…”

Section: Discussionmentioning

confidence: 99%

Limited protection of rabbits against infection with Treponema pallidum by immune rabbit sera.

Graves¹,

Alden²

1979

Sexually Transmitted Infections

View full text Add to dashboard Cite

SUMMARY After intradermal infection of rabbits with 3 x 106 Treponemapallidum (Melbourne 1 strain) samples of serum were taken at one, two, three, four, and six months after infection.Normal rabbits were passively immunised with these sera, challenged with intradermal doses (104, 103, 102, 10) of T. pallidum, and the latent periods of infection, lesion diameters, and the number of inoculation sites developing into lesions were observed. The sera taken at three, four, and six months reduced the number of intradermal inoculation sites that developed into syphilitic lesions after challenge with 10 T. pallidum. These same three sera also increased the latent period of infection after challenge with 104 T. pallidum. The transfer of 50 ml of immune serum per rabbit over a nine-day period before challenge had very little effect on the course of the challenge infection. Only a low level of immunity in rabbits to this strain of T. pallidum appears to be mediated by immune serum but this small degree of protection did increase with time after infection. Enhanced growth of T. pallidum in the serum-recipient rabbits did not occur, thus suggesting that none of the sera was immunosuppressive.

show abstract

Section: Discussionmentioning

confidence: 99%

Limited protection of rabbits against infection with Treponema pallidum by immune rabbit sera.

Graves¹,

Alden²

1979

Sexually Transmitted Infections

View full text Add to dashboard Cite

show abstract

“…This finding initially raised the possibility that most of the specific anti-T. pallidum hybridomas may be directed against intracellular rather than surface antigens of the T. pallidum organism. However, it has not been determined whether the sonication process releases intracellular T. pallidum components (against which the monoclonal antibodies are directed), or if sonication actually exposes or "unmasks" surface determinants of T. pallidum that are normally unexposed or coated with host proteins (1) or mucopolysaccharide (11,12,14,15,20,25,60,65). Indeed, the previously observed binding of these components to the surface of T. pallidum may be an important pathogenetic mechanism that protects the organism from the immune response of the host (16, 17, 30,33,35,38).…”

Section: Igg2bmentioning

confidence: 99%

Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols)

Robertson¹,

Kettman²,

Miller³

et al. 1982

Infect Immun

View full text Add to dashboard Cite

Murine anti-Treponema pallidum (Nichols) lymphocyte hybridoma cell lines secreting monoclonal antibodies against a variety of treponemal antigens have been generated. Hybridomas isolated were of three major types: those that were directed specifically against T. pallidum antigens, those that were directed against treponemal group antigens (as evidenced by their cross-reactivity with T. phagedenis biotype Reiter antigens), and those that cross-reacted with both treponemal as well as rabbit host testicular tissue antigens. The majority (31 of 39 clones) of these anti-T. pallidum hybridomas, which produced monoclonal antibodies of mouse isotypes immunoglobulin G1 (IgG1), IgG2a, IgG2b, IgG3 or IgM, were directed specifically against T. pallidum and not other treponemal or rabbit antigens tested by radioimmunoassay. Four of these T. pallidum-specific hybridomas secreted monoclonal antibodies with greater binding affinity for "aged" rather than freshly isolated intact T. pallidum cells, suggesting a possible specificity for "unmasked" surface antigens of T. pallidum. Six anti-T. pallidum hybridomas produced complement-fixing monoclonal antibodies (IgG2a, IgG2b, or IgM) that were capable of immobilizing virulent treponemes in the T. pallidum immobilization (TPI) test; these may represent biologically active monoclonal antibodies against treponemal surface antigens. Three other hybridomas secreted monoclonal antibodies which bound to both T. pallidum and T. phagedenis biotype Reiter antigens, thus demonstrating a possible specificity for treponemal group antigens. Five hybridoma cell lines were also isolated which produced IgM monoclonal antibodies that cross-reacted with all treponemal and rabbit host testicular tissue antigens employed in the radioimmunoassays. This report describes the construction and characteristics of these hybridoma cell lines. The potential applications of the anti-T. pallidum monoclonal antibodies are discussed.

show abstract

“…Although T. pallidum carries host immunoglobulins on its surface (1,25), the anti-ITF antibodies could not have been directed to rabbit allotypes, as the animals used for ITF production and subsequent immunization were of the same allotype (Ab4/Ab4). We have further tested rabbit antisera to various rabbit allotypes and found that only rabbit antiserum to allotype 5 reacts with T. pallidum on commercial slides.…”

Section: Discussionmentioning

confidence: 99%

Antigenic evidence for host origin of exudative fluids in lesions of Treponema pallidum-infected rabbits

Wos¹,

Wicher²

1985

Infect Immun

View full text Add to dashboard Cite

Mucoid fluid accumulating within syphilitic lesions has been considered to be of Treponema pallidum origin. To test this assumption, we examined testicular exudative fluids from T. pallidum-infected rabbits for the presence of T. pallidum antigens by various sensitive immunochemical methods, including Western blot analysis. Antigenic analysis of these fluids revealed host components but not treponemal antigens. Prolonged immunization of rabbits, guinea pigs, and a goat with this material in complete Freund adjuvant elicited low titers (fluorescent-treponemal-antibody test titer, .10) of antitreponemal antibodies in the rabbits and guinea pigs but not in the goat. The data suggest that these mucoid fluids are of host origin. The presence of mucopolysaccharides in these fluids may be related to the infective process. The possible mechanism by which mucopolysaccharides protect T. pallidum from immune mechanisms and its potential relationship to the pathogenesis of the disease are discussed. 228on July 16, 2020 by guest

show abstract

Rabbit globulin and antiglobulin factors associated with Treponema pallidum growth in rabbits.

Cited by 13 publications

References 18 publications

Limited protection of rabbits against infection with Treponema pallidum by immune rabbit sera.

Limited protection of rabbits against infection with Treponema pallidum by immune rabbit sera.

Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols)

Antigenic evidence for host origin of exudative fluids in lesions of Treponema pallidum-infected rabbits

Contact Info

Product

Resources

About