Rab35 is translocated from Arf6-positive perinuclear recycling endosomes to neurite tips during neurite outgrowth

Kobayashi, Hotaka; Etoh, Kan; Fukuda, Mitsunori

doi:10.4161/sgtp.29290

Cited by 23 publications

(21 citation statements)

References 31 publications

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“…Interestingly, Rab35 colocalizes with Cdc42, Rac1 and RhoA in PC12 and N1E‐115 and BHK neuronal cells, activates Cdc42 and stimulates neurite outgrowth in a Cdc42‐dependent manner through actin remodeling . In addition, Rab35 translocates from recycling endosomes to neurite tips upon NGF stimulation . Finally, Rab35 also regulates Rac activity in Wnt5‐induced migration , as well as Rac and Cdc42 localization during phagocytosis in Drosophila .…”

Section: Rab35: a Key Regulator Of F‐actin At The Plasma Membranementioning

confidence: 99%

Rab35 GTPase: A Central Regulator of Phosphoinositides and F‐actin in Endocytic Recycling and Beyond

Klinkert

Échard

2016

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Rab35 is one of the first discovered members of the large Rab GTPase family, yet it received little attention for 10 years being considered merely as a Rab1-like GTPase. In 2006, Rab35 was recognized as a unique Rab GTPase localized both at the plasma membrane and on endosomes, playing essential roles in endocytic recycling and cytokinesis. Since then, Rab35 has become one of the most studied Rabs involved in a growing number of cellular functions, including endosomal trafficking, exosome release, phagocytosis, cell migration, immunological synapse formation and neurite outgrowth. Recently, Rab35 has been acknowledged as an oncogenic GTPase with activating mutations being found in cancer patients. In this review, we provide a comprehensive summary of known Rab35-dependent cellular functions and detail the few Rab35 effectors characterized so far. We also review how the Rab35 GTP/GDP cycle is regulated, and emphasize a newly discovered mechanism that controls its tight activation on newborn endosomes. We propose that the involvement of Rab35 in such diverse and apparently unrelated cellular functions can be explained by the central role of this GTPase in regulating phosphoinositides and F-actin, both on endosomes and at the plasma membrane.

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Section: Rab35: a Key Regulator Of F‐actin At The Plasma Membranementioning

confidence: 99%

Rab35 GTPase: A Central Regulator of Phosphoinositides and F‐actin in Endocytic Recycling and Beyond

Klinkert

Échard

2016

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“…The transcytosis of TrkA is dependent on Rab11 recycling endosomes and is promoted by NGF signaling itself at the axon terminal, establishing a positive feedback network to replenish TrkA at the axonal growth cone and amplify NGF signal transduction (Ascano et al, 2009). Recent studies have demonstrated that EHD1 proteins play a role of “gatekeeper” in the endosome trafficking system for neurite outgrowth of PC12 cells (Kobayashi et al, 2014). EHD1 regulates and controls the endocytosis and transportation of cargos such as NgCAM or membrane recepter via recycling endosome (Lasiecka et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

The importance of EHD1 in neurite outgrowth contributing to the functional recovery after spinal cord injury

Cui

Liu³

et al. 2016

Intl J of Devlp Neuroscience

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Traumatic spinal cord injury is one of the most common and severe problems for using NGF to promote the neurite outgrowth of survival neurons. EHD1 regulates and controls the endocytosis and transportation of neurotrophins and transmembrane cargo via recycling endosome for neurite outgrowth. TrkA is particularly considered to be a functional specific recepter in the cell membrane for NGF and is activated upon NGF binding. The transcytosis of TrkA is dependent on Rab11 recycling endosomes and is promoted by NGF signaling itself at the axon terminal. In this study, we established an acute spinal cord contusion injury model in adult rats to investigate the potential role of EHD1 during the pathological process of SCI. Western blot analysis suggested that EHD1 expression was low in the sham-operated adult rat spinal cords and was significantly up-regulated 1d after injury. Immunohistochemical staining detected the general distribution of EHD1 protein in both the gray and white matter of adult rat spinal cords. Double immunofluorescent staining indicated that EHD1 was expressed in neurons, astrocytes and microglias in the adult rat spinal cord, and obvious changes of EHD1 expression occurred in neurons during SCI pathological process. Significant up-regulation of EHD1 expression was observed in MAP2 positive neurons at 1 day after SCI, in comparison with the sham-operated control, which indicated that EHD1 might play a vital role in neurite outgrowth. Our data indicated that EHD1 could interact with TrkA, and is in the upstream of TrkA. EHD1 up-regulated the expression of TrkA in the glutamate stimulated primary neurons. Based on our experimental data, we boldly conclude that EHD1 regulates the recycling of TrkA back to cell membrane, improving the utilization efficiency of the NGF, which is vital for neurite outgrowth and functional recovery after spinal cord injury.

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“…By contrast, the RE resident GTPase, Arf6 negatively regulates axon outgrowth and trafficking of β1‐integrin‐containing RE (Hernández‐Deviez et al, ; Eva et al, ). During neurite outgrowth Rab35 recruits its effector, the GTPase‐activating proteins (GAPs) centaurin‐β2 that inhibits Arf6 activity (Kobayashi and Fukuda, ) and MICAL‐L1 which functions as a scaffold protein for Rab8, Rab13, and Rab36, resulting in multiple Rab signalling at the same time (Kobayashi et al, ,b). Taken together, multiple RabGTPases and Arf6 activities cooperatively regulate trafficking of RE to control neuronal differentiation and axonal growth axon development.…”

Section: Membrane Addition By the Recycling Endosome In Growing Axonsmentioning

confidence: 99%

Regulation of plasma membrane expansion during axon formation

Quiroga

Bisbal

Cáceres

2017

Developmental Neurobiology

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Rab35 is translocated from Arf6-positive perinuclear recycling endosomes to neurite tips during neurite outgrowth

Cited by 23 publications

References 31 publications

Rab35 GTPase: A Central Regulator of Phosphoinositides and F‐actin in Endocytic Recycling and Beyond

Rab35 GTPase: A Central Regulator of Phosphoinositides and F‐actin in Endocytic Recycling and Beyond

The importance of EHD1 in neurite outgrowth contributing to the functional recovery after spinal cord injury

Regulation of plasma membrane expansion during axon formation

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