Rab27A Is An Independent Prognostic Factor in Clear Cell Renal Cell Carcinoma

An, Hyo Jung; Song, Dae Hyun; Koh, Hyun Min; Ko, Gyung Hyuck; Lee, Jeong-Hee; Kim, Dong Chul; Yang, Jung Wook; Kim, Min Hye; Seo, Deok Ha; Jang, Sang Ock; Lee, Jong Sil

doi:10.2217/bmm-2018-0336

Cited by 10 publications

(13 citation statements)

References 17 publications

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“…Tumor cells secrete exosomes with intra-exosomal cargos, including tumor-suppressor genes, proteins, and other substances that might affect metastatic properties. To date, there have been many studies regarding the prognostic role of RAB27A and RAB27B through immunostaining methods in various cancer (12)(13)(14)(15)(16)(17)(18). In a previous study, we confirmed that RAB27A expression was strongly positive in a large group of patients with clearcell renal cell carcinoma (CCRCC) (13).…”

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confidence: 53%

RAB27A and RAB27B Expression May Predict Lymph Node Metastasis and Survival in Patients With Gastric Cancer

Lee

Yang

et al. 2022

Cancer Genomics Proteomics

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Background/Aim: RAB27A and RAB27B are involved in exosome secretion. To date, there have been many attempts to elucidate the roles of RAB27A and RAB27B in the prognosis of various cancer types. The association of RAB27A and RAB27B expression with the clinical and pathological features was evaluated in patients with stomach cancer. Materials and Methods: A total of 360 patients who had undergone surgery for stomach cancer between January 1999 and December 2007 at Gyeongsang National University were enrolled in the study. Disease-free survival (DFS) and disease-specific survival (DSS) were compared according to immunohistochemistry of tumor samples. RAB27A and RAB27B mRNA and protein were also extracted from four stomach cancer cell lines using quantitative polymerase chain reaction and western blotting. Results: Strong nuclear RAB27A expression in tumor samples was statistically significantly correlated with lymph node metastasis. Cytoplasmic RAB27B expression was related to poor disease-free survival and its combined cytoplasmic and membranous expression was related to disease-specific survival of patients with different histopathological types of stomach cancer. High RAB27A expression and high RAB27B expression was found in four stomach cancer cell blocks. Among the four cell lines, NCI-N87 exhibited the lowest relative mRNA density and HS746T exhibited the highest relative protein density for both RAB27A and RAB27B. Conclusion: RAB27A and RAB27B expression may help predict lymph node metastasis and survival of patients with gastric cancer.

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confidence: 53%

RAB27A and RAB27B Expression May Predict Lymph Node Metastasis and Survival in Patients With Gastric Cancer

Lee

Yang

et al. 2022

Cancer Genomics Proteomics

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“…Rab27B could provide proliferative and metastatic ability in estrogen receptor-positive breast cancer cells. 12 Similarly, Rab27B knockdown remarkably inhibited invasion and proliferation in human pancreatic cancer cells. 20 Koh et al found that up-regulated expression of Rab27B was significantly related to unfavorable prognosis in lung squamous cell cancers, pancreatic cancer, gastrointestinal tumors, and ovarian cancer.…”

Section: Discussionmentioning

confidence: 96%

“…The family mainly consists of two major members, Rab27A and Rab27B, which have been investigated closely associated with tumor malignancy and can be implied as a prognostic indicator in several cancers, including non-small cell lung cancer, colorectal cancer, hepatocellular carcinoma, clear cell renal cell carcinoma, and pancreatic cancer. [10][11][12][13] However, the prognostic value of Rab27A and Rab27B in ESCC remains unknown. In the current study, we detected the expression levels of Rab27A and Rab27B, and explored their correlation with clinical characteristics and prognostic significance in ESCC.…”

Section: Introductionmentioning

confidence: 99%

<p>Prognostic Significance of Rab27A and Rab27B Expression in Esophageal Squamous Cell Cancer</p>

Yu¹,

Wu²,

Liang³

et al. 2020

CMAR

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Rab27A and Rab27B, members of the Rab family of small GTPases, have aberrant expression and exert different roles in various cancers. However, their expression and potential prognostic values in esophageal squamous cell cancer (ESCC) still remain elusive. In the present study, we explored the association of Rab27A and Rab27B expression with clinical significance and prognosis in ESCC. Patients and Methods: A total of 100 surgically resected ESCC tissues were examined to evaluate Rab27A and Rab27B expression levels using the immunohistochemistry method. The relationship of Rab27A and Rab27B with clinicopathological features and prognosis was analyzed. We also investigated the correlation between Rab27A and Rab27B through external and internal validation. Results: High-expression Rab27A was found to be significantly correlated with N (p=0.045) and TNM (p=0.005) stage, while up-regulated Rab27B was remarkably associated with N stage (p=0.033), TNM stage (p=0.009), and differentiation (p=0.013). High expression of both Rab27A and Rab27B had a worse overall survival (OS) rate. In addition, multivariate Cox regression analyses were utilized to validate that Rab27B expression is an independent prognostic factor for unfavorable OS. Further combined analyses showed that the Rab27A low /B low group had a superior OS rate than the Rab27A high /B low group, Rab27A low / B high group, and Rab27A high /B high group. Nevertheless, the latter three groups displayed rare significance between each two comparisons. Furthermore, our data demonstrated that Rab27A expression was positively correlated with Rab27B expression, which were also verified in TCGA datasets. Conclusion: Rab27A and Rab27B expression levels could be potentially novel prognostic biomarkers in ESCC.

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“…Previous studies have demonstrated that RAB27A was highly up-regulated and acts as a valuable prognostic indicator in various cancers, including hepatocellular carcinoma [25] , gliomas [20] , esophageal squamous cell cancer [26] and clear cell renal cell carcinoma [27] .However, it is controversial for RAB27A expression in colon cancer tumor tissues. It has been reported that RAB27A highly expressed in CRC tumor tissues compared with matched adjacent tissues [21] .…”

Section: Discussionmentioning

confidence: 99%

RAB27A Promotes the Proliferation and Invasion of Colorectal Cancer Cells

Zhao

Dong

et al. 2022

Preprint

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Colorectal cancer (CRC) is the most commonly diagnosed form of cancer worldwide. Though significant advances in prevention and diagnosis, CRC is still one of the leading causes of cancer-related mortality globally. RAB27A, the member of RAB27 family of small GTPases, is the critical protein for intracellular secretion and was reported to promote tumor progression. However, it is controversial for the role of RAB27A in CRC progression, so we explored the exact function of RAB27A in CRC development in this study. Based on the stable colon cancer cell lines of RAB27A knockdown and ectopic expression, we found that RAB27A knockdown inhibited SW480 colon cancer cell proliferation and clone formation, whereas ectopic expression of RAB27A in RKO colon cancer cells facilitated cell proliferation and clone formation, indicating that RAB27A is critical for colon cancer cell growth. In addition, our data demonstrated that the migration and invasion of colon cancer cells were suppressed by RAB27A knockdown, but promoted by RAB27A ectopic expression. Therefore, RAB27A was identified as an onco-protein in mediating CRC development, which may be a valuable prognostic indicator and potential therapeutic target for CRC.

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Rab27A Is An Independent Prognostic Factor in Clear Cell Renal Cell Carcinoma

Cited by 10 publications

References 17 publications

RAB27A and RAB27B Expression May Predict Lymph Node Metastasis and Survival in Patients With Gastric Cancer

RAB27A and RAB27B Expression May Predict Lymph Node Metastasis and Survival in Patients With Gastric Cancer

<p>Prognostic Significance of Rab27A and Rab27B Expression in Esophageal Squamous Cell Cancer</p>

RAB27A Promotes the Proliferation and Invasion of Colorectal Cancer Cells

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