Rab-mediated vesicular transport is required for neuronal positioning in the developing Drosophila visual system

Houalla, Tarek; Shi, Lei; Meyel, Donald J. van; Rao, Yong

doi:10.1186/1756-6606-3-19

Cited by 10 publications

(12 citation statements)

References 73 publications

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“…Whether the defect in forward nuclear movement of migrating neurons is secondary to the abnormal adhesion or whether Rab5 and Rab11 directly regulate nuclear movement is still unclear. A very recent report showed that Rab5 and Rab11 are required for maintaining apical localization of the nuclei in Drosophila photoreceptor cells, 30 suggesting that Rab5-and Rab11-dependent pathways might be directly involved in nuclear movement.…”

Section: Rab11 and Rab7 Regulate Distinct Steps Of Neuronal Migrationmentioning

confidence: 99%

Regulation of cell adhesion and migration in cortical neurons

Kawauchi

2011

Small GTPases

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Section: Rab11 and Rab7 Regulate Distinct Steps Of Neuronal Migrationmentioning

confidence: 99%

Regulation of cell adhesion and migration in cortical neurons

Kawauchi

2011

Small GTPases

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“…While the role of Rab6 in membrane trafficking is well established, very few GAPs or GEFs for Rab6 have been identified; thus their physiological importance is largely unknown. The physiological roles of RabGAPs were addressed only recently in flies (Houalla et al 2010;Uytterhoeven et al 2011) and worms (Chotard et al 2010). In those cases, removing the function of the RabGAPs produced phenotypes almost identical to those of removing the relevant Rabs, showing the fundamental roles of RabGAPs in modulating Rab functions.…”

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confidence: 99%

The Jaw of the Worm: GTPase-activating Protein EAT-17 Regulates Grinder Formation in Caenorhabditis elegans

et al. 2013

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Constitutive transport of cellular materials is essential for cell survival. Although multiple small GTPase Rab proteins are required for the process, few regulators of Rabs are known. Here we report that EAT-17, a novel GTPase-activating protein (GAP), regulates RAB-6.2 function in grinder formation in Caenorhabditis elegans. We identified EAT-17 as a novel RabGAP that interacts with RAB-6.2, a protein that presumably regulates vesicle trafficking between Golgi, the endoplasmic reticulum, and plasma membrane to form a functional grinder. EAT-17 has a canonical GAP domain that is critical for its function. RNA interference against 25 confirmed and/or predicted RABs in C. elegans shows that RNAi against rab-6.2 produces a phenotype identical to eat-17. A directed yeast twohybrid screen using EAT-17 as bait and each of the 25 RAB proteins as prey identifies RAB-6.2 as the interacting partner of EAT-17, confirming that RAB-6.2 is a specific substrate of EAT-17. Additionally, deletion mutants of rab-6.2 show grinder defects identical to those of eat-17 loss-of-function mutants, and both RAB-6.2 and EAT-17 are expressed in the terminal bulb of the pharynx where the grinder is located. Collectively, these results suggest that EAT-17 is a specific GTPase-activating protein for RAB-6.2. Based on the conserved function of Rab6 in vesicular transport, we propose that EAT-17 regulates the turnover rate of RAB-6.2 activity in cargo trafficking for grinder formation. C ELLS constitutively transport newly synthesized proteins, lipids, and other molecules to their periphery through vesicle trafficking. As the process is conserved from yeast to humans, it is essential for the function and survival of cells. Rab GTPases are small GTP binding proteins that regulate the transport of vesicles between different compartments of the cell (Zerial and McBride 2001;Jordens et al. 2005;Grosshans et al. 2006). Rab6's are localized to Golgi membranes to mark and target both anterograde cargos from Golgi to post-Golgi compartments (such as the plasma membrane) and retrograde cargos from early/recycling endosomes to Golgi and the endoplasmic reticulum (Jasmin et al. 1992;Martinez et al. 1994Martinez et al. , 1997Girod et al. 1999;Opdam et al. 2000;Del Nery et al. 2006). For fast turnover, Rabs require guanine nucleotide exchange factors (Rab GEFs) for activation and GTPase-activating proteins (Rab GAPs) to turn off activity (Grosshans et al. 2006). While the role of Rab6 in membrane trafficking is well established, very few GAPs or GEFs for Rab6 have been identified; thus their physiological importance is largely unknown. The physiological roles of RabGAPs were addressed only recently in flies (Houalla et al. 2010;Uytterhoeven et al. 2011) and worms (Chotard et al. 2010). In those cases, removing the function of the RabGAPs produced phenotypes almost identical to those of removing the relevant Rabs, showing the fundamental roles of RabGAPs in modulating Rab functions. Currently, the only identified GAP for Rab6 is GAPCenA in humans,...

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“…Lsp2 is a subunit of the Drosophila hexamerin protein; it functions during differentiation of the mosquito larval–pupa l stage [ 33 ]. The RN-tre gene product is thought to interact with Rab5 and Rab11 to regulate apical localization of the R-cell [ 34 ]. Ets family members are expressed in Drosophila oocytes and larvae, and are involved in cell migration [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Chronic low-dose -irradiation of Drosophila melanogaster larvae induces gene expression changes and enhances locomotive behavior

Kim¹,

Seong

Lee³

et al. 2015

Journal of Radiation Research

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Although radiation effects have been extensively studied, the biological effects of low-dose radiation (LDR) are controversial. This study investigates LDR-induced alterations in locomotive behavior and gene expression profiles of Drosophila melanogaster. We measured locomotive behavior using larval pupation height and the rapid iterative negative geotaxis (RING) assay after exposure to 0.1 Gy γ-radiation (dose rate of 16.7 mGy/h). We also observed chronic LDR effects on development (pupation and eclosion rates) and longevity (life span). To identify chronic LDR effects on gene expression, we performed whole-genome expression analysis using gene-expression microarrays, and confirmed the results using quantitative real-time PCR. The pupation height of the LDR-treated group at the first larval instar was significantly higher (∼2-fold increase in PHI value, P < 0.05). The locomotive behavior of LDR-treated male flies (∼3 − 5 weeks of age) was significantly increased by 7.7%, 29% and 138%, respectively (P < 0.01), but pupation and eclosion rates and life spans were not significantly altered. Genome-wide expression analysis identified 344 genes that were differentially expressed in irradiated larvae compared with in control larvae. We identified several genes belonging to larval behavior functional groups such as locomotion (1.1%), oxidation reduction (8.0%), and genes involved in conventional functional groups modulated by irradiation such as defense response (4.9%), and sensory and perception (2.5%). Four candidate genes were confirmed as differentially expressed genes in irradiated larvae using qRT-PCR (>2-fold change). These data suggest that LDR stimulates locomotion-related genes, and these genes can be used as potential markers for LDR.

show abstract

Rab-mediated vesicular transport is required for neuronal positioning in the developing Drosophila visual system

Cited by 10 publications

References 73 publications

Regulation of cell adhesion and migration in cortical neurons

Regulation of cell adhesion and migration in cortical neurons

The Jaw of the Worm: GTPase-activating Protein EAT-17 Regulates Grinder Formation in Caenorhabditis elegans

Chronic low-dose -irradiation of Drosophila melanogaster larvae induces gene expression changes and enhances locomotive behavior

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