Rab GTPases and cell division

Gibieža, Paulius; Prekeris, Rytis

doi:10.1080/21541248.2017.1313182

Cited by 19 publications

(18 citation statements)

References 44 publications

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“…6A; Supplemental Table S1), which belong to a family of monomeric small GTPases of the Ras superfamily. It has been reported that several Rab GTPases are crucial for cytokinesis and function in regulating cell division (Qi and Zheng, 2013;Gibie za and Prekeris, 2018). Therefore, the response of root elongation in rapeseed to N deficiency might be achieved through a coordination of these relevant proteins involved in both cell division and cell expansion.…”

Section: Discussionmentioning

confidence: 99%

Adaption of Roots to Nitrogen Deficiency Revealed by 3D Quantification and Proteomic Analysis

et al. 2018

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Rapeseed (Brassica napus) is an important oil crop worldwide. However, severe inhibition of rapeseed production often occurs in the field due to nitrogen (N) deficiency. The root system is the main organ to acquire N for plant growth, but little is known about the mechanisms underlying rapeseed root adaptions to N deficiency. Here, dynamic changes in root architectural traits of N-deficient rapeseed plants were evaluated by 3D in situ quantification. Root proteome responses to N deficiency were analyzed by the tandem mass tag-based proteomics method, and related proteins were characterized further. Under N deficiency, rapeseed roots become longer, with denser cells in the meristematic zone and larger cells in the elongation zone of root tips, and also become softer with reduced solidity. A total of 171 and 755 differentially expressed proteins were identified in shortand long-term N-deficient roots, respectively. The abundance of proteins involved in cell wall organization or biogenesis was highly enhanced, but most identified peroxidases were reduced in the N-deficient roots. Notably, peroxidase activities also were decreased, which might promote root elongation while lowering the solidity of N-deficient roots. These results were consistent with the cell wall components measured in the N-deficient roots. Further functional analysis using transgenic Arabidopsis (Arabidopsis thaliana) plants demonstrated that the two root-related differentially expressed proteins contribute to the enhanced root growth under N deficiency conditions. These results provide insights into the global changes of rapeseed root responses to N deficiency and may facilitate the development of rapeseed cultivars with high N use efficiency through rootbased genetic improvements.

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Section: Discussionmentioning

confidence: 99%

Adaption of Roots to Nitrogen Deficiency Revealed by 3D Quantification and Proteomic Analysis

et al. 2018

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“…Ras-related protein 6 (RAB6) is a kind of small GTPases which are involved in vesicular traffic in the secretory and endocytic pathways 15 . Rab protein, as a molecular switch of vesicle transport, interacts with its upstream regulatory factors and downstream specific effectors, plays an important role in the formation and transport of vesicles, and a series of Rab effector proteins have been found to be involved in cellular phagocytic and protein transport pathways 16 – 18 . RAB6 is involved in the composition of the Golgi and cytoplasmic vesicles and regulate protein transport between the Golgi apparatus, endoplasmic reticulum (ER) and endosomes 19 , 20 .…”

Section: Introductionmentioning

confidence: 99%

Small GTPase RAB6 deficiency promotes alveolar progenitor cell renewal and attenuates PM2.5-induced lung injury and fibrosis

Yang

Liu

et al. 2020

Cell Death Dis

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Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease characterized by chronic non-specific inflammation of the interstitial lung and extensive deposition of collagen fibers leading to destruction of lung function. Studies have demonstrated that exposure to fine particulate matter (PM2.5) increases the risk of IPF. In order to recover from PM2.5-induced lung injury, alveolar epithelial cells need to be repaired and regenerated to maintain lung function. Type 2 alveolar epithelial cells (AEC2) are stem cells in the adult lung that contribute to the lung repair process through complex signaling. Our previous studies demonstrated that RAB6, a RAS family member lowly expressed in lung cancer, inhibited lung cancer stem cell self-renewal, but it is unclear whether or not and how RAB6 may regulate AEC2 cell proliferation and self-renewal in PM2.5-induced pulmonary fibrosis. Here, we demonstrated that knockout of RAB6 inhibited pulmonary fibrosis, oxidative stress, and AEC2 cell death in PM2.5-injured mice. In addition, knockout of RAB6 decreased Dickkopf 1(DKK1) autocrine and activated proliferation, self-renewal, and wnt/β-catenin signaling of PM2.5-injured AEC2 cells. RAB6 overexpression increased DKK1 autocrine and inhibited proliferation, self-renewal and wnt/β-catenin signaling in AEC2 cells in vitro. Furthermore, DKK1 inhibitors promoted proliferation, self-renewal and wnt/β-catenin signaling of RAB6 overexpressing AEC2 cells, and attenuated PM2.5-induced pulmonary fibrosis in mice. These data establish RAB6 as a regulator of DKK1 autocrine and wnt/β-catenin signal that serves to regulate AEC2 cell proliferation and self-renewal, and suggest a mechanism that RAB6 disruption may promote AEC2 cell proliferation and self-renewal to enhance lung repair following PM2.5 injury.

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“…Its increased abundance is associated with a higher oxygen supply to gills of trachurus fish, which supports oxidative metabolism and the larger growth phenotype. Rab-GDI regulates Rab GTPases, which control many cellular signaling processes, including those that are involved in membrane trafficking, cell differentiation and proliferation (growth) 23 .…”

Section: Morphotype Fingerprint Proteinsmentioning

confidence: 99%

Identification of key proteins involved in stickleback environmental adaption with system-level analysis

Hall

Kueltz

Almaas

2020

Preprint

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Using abundance measurements of 1,490 proteins from four separate populations of three-spined sticklebacks, we implemented a system-level approach to correlate proteome dynamics with environmental salinity and temperature and the fish's population and morphotype. We identified sets of robust and accurate fingerprints that predict environmental salinity, temperature, morphotype and the population sample origin, observing that proteins with specific functions are enriched in these fingerprints. Highly apparent functions represented in all fingerprints include ion transport, proteostasis, growth, and immunity, suggesting that these functions are most diversified in populations inhabiting different environments. Applying a differential network approach, we analyzed the network of protein interactions that differs between populations. Looking at specific population combinations of differential interaction, we identify sets of connected proteins. We find that these sets and their corresponding enriched functions reflect key processes that have diverged between the four populations. Moreover, the extent of divergence, i.e. the number of enriched functions that differ between populations, is highest when all three environmental parameters are different between two populations. Key nodes in the differential interaction network signify functions that are also inherent in the fingerprints, most prominently proteostasis-related functions. However, the differential interaction network also reveals additional functions that have diverged between populations, notably cytoskeletal organization and morphogenesis. Having such a large proteomic dataset, the strength of these analyses is that the results are purely data-driven, not based on previous findings and hypotheses about adaptation. With such an unbiased approach applied on a large proteomic dataset, we find the strongest signals given by the data, making it possible to develop more discriminatory and complex biomarkers for specific contexts of interest.

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Rab GTPases and cell division

Cited by 19 publications

References 44 publications

Adaption of Roots to Nitrogen Deficiency Revealed by 3D Quantification and Proteomic Analysis

Adaption of Roots to Nitrogen Deficiency Revealed by 3D Quantification and Proteomic Analysis

Small GTPase RAB6 deficiency promotes alveolar progenitor cell renewal and attenuates PM2.5-induced lung injury and fibrosis

Identification of key proteins involved in stickleback environmental adaption with system-level analysis

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