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勲, 渋谷

doi:10.1246/nikkashi.1979.389

Cited by 9 publications

(1 citation statement)

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“…[3a] Secondly the GL-7-ACA, formed in the previous step, is hydrolyzed to 7-ACA and glutaric acid by an enzyme named glutaryl acylase (GAC) (Scheme 1). [4] The principal drawback of this process for the production of 7-ACA is the presence of hydrogen peroxide during the reaction, because hydrogen peroxide can inactivate the enzymes employed in the reaction, especially DAAO. [5] Many attempts have been carried out to avoid the inactivation of DAAO and GAC by hydrogen peroxide through different immobilization strategies but with only moderate success.…”

Section: Introductionmentioning

confidence: 99%

One‐Pot Conversion of Cephalosporin C to 7‐Aminocephalosporanic Acid in the Absence of Hydrogen Peroxide

et al. 2005

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Abstract:The main drawback in the production of 7-aminocephalosporanic acid (7-ACA) at the industrial level is the inactivation of the enzymes implicated in the process due to the presence of hydrogen peroxide during the reaction. As an alternative, we have developed the conversion of cephalosporin C to 7-ACA in a single reactor without the presence of hydrogen peroxide during the reaction, achieving more than 80% yield. In order to develop this process, d-amino acid oxidase (DAAO) was co-immobilized with catalase (CAT), which is able to fully eliminate in situ the hydrogen peroxide formed by the neighbouring DAAO molecules. Thus, the product of the reaction is only aketoadipyl-7-ACA. This system prevents the inactivation of the oxidase by hydrogen peroxide, solving the main problem of the enzymatic process. Moreover, we have found that a-ketoadipyl-7-ACA is recognized as a substrate by glutaryl acylase (GAC) and hydrolyzed as long as glutaric acid is absent from the reaction medium (because it is able to inhibit the hydrolysis). The low stability of a-ketoadipyl-7-ACA justifies the use of a single reactor, in which glutaryl acylase is already present when this substrate is generated. Thus, the whole process may (and must) be performed in a single step, and in the absence of hydrogen peroxide that could affect the stabilities of the involved enzymes.

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Section: Introductionmentioning

confidence: 99%