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Bhattacharjee, Paramita; Shukla, V.B.; Kulkarni, P. R.

doi:10.1023/a:1013573912952

Cited by 56 publications

(24 citation statements)

References 12 publications

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“…Since its discovery, shark liver oil has been the largest source of squalene. A wide range of microorganisms including yeast such as Saccharomyces cerevisiae (Mantzouridou and Tsimidou, 2010) and Torulaspora delbrueckii (Bhattacharjee et al, 2001), fungus Aurantiochytrium sp. (Jiang et al, 2004; Nakazawa et al, 2014; Pora et al, 2014), Euglena (Anding et al, 1971), archaea Halobacterium cutirubrum , and several other species have the natural ability to produce squalene.…”

Section: Introductionmentioning

confidence: 99%

Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities

Gohil

Bhattacharjee

Khambhati

et al. 2019

Front. Bioeng. Biotechnol.

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The triterpene squalene is a natural compound that has demonstrated an extraordinary diversity of uses in pharmaceutical, nutraceutical, and personal care industries. Emboldened by this range of uses, novel applications that can gain profit from the benefits of squalene as an additive or supplement are expanding, resulting in its increasing demand. Ever since its discovery, the primary source has been the deep-sea shark liver, although recent declines in their populations and justified animal conservation and protection regulations have encouraged researchers to identify a novel route for squalene biosynthesis. This renewed scientific interest has profited from immense developments in synthetic biology, which now allows fine-tuning of a wider range of plants, fungi, and microorganisms for improved squalene production. There are numerous naturally squalene producing species and strains; although they generally do not make commercially viable yields as primary shark liver sources can deliver. The recent advances made toward improving squalene output from natural and engineered species have inspired this review. Accordingly, it will cover in-depth knowledge offered by the studies of the natural sources, and various engineering-based strategies that have been used to drive the improvements in the pathways toward large-scale production. The wide uses of squalene are also discussed, including the notable developments in anti-cancer applications and in augmenting influenza vaccines for greater efficacy.

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Section: Introductionmentioning

confidence: 99%

Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities

Gohil

Bhattacharjee

Khambhati

et al. 2019

Front. Bioeng. Biotechnol.

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“…Chloroform being a lesser polar solvent and methanol being a polar solvent enhances the damage of cell wall thereby aiding in the release of squalene from the cells (Park et al 2015). Chloroform-methanol solvent of 2:1 ratio has shown better squalene recovery when compared with other solvents such as petroleum ether and chloroform (Bhattacharjee et al 2001). The same solvent system has also been used for squalene extraction from euglena (Kawaura et al 1995).…”

Section: Discussionmentioning

confidence: 99%

“…Kamimura et al (1994) has pioneered the gene disruption in ergosterol biosynthesis pathway for squalene accumulation and has reached a squalene yield of 5 mg/g dry cell weight by random mutation in ERG1 gene. Bhattacharjee et al (2001) has produced squalene in a commercial S. cerevisiae strain with the yield of 41 μg/g DCW under anaerobic fermentative conditions. Garaiová et al (2014) has studied the squalene enhancement in ERG1 mutant strains in the presence of ergosterol biosynthesis inhibitor, terbinafine and has achieved a squalene yield of 1000 μg per 10 9 cells.…”

Section: Introductionmentioning

confidence: 99%

Studies on squalene biosynthesis and the standardization of its extraction methodology fromSaccharomyces cerevisiae

Paramasivan

Mutturi

Rajagopal

2018

Preprint

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Microbiology and 1 2 3 1 laboratory S. cerevisiae strains such as BY4742 and CEN.PK2-1C (native), deletion strains 3 2 (ERG6 and ERG11) and tHMG1 overexpressed S. cerevisiae strains. When sonication method of 3 3cell lysis was replaced with homogenization it was found that the yields were significantly higher 3 4and reached a value of 9 mg/g DCW in case of BY4742. In addition, Squalene yield in ergosterol 3 5 mutant strains has been analyzed and was found to be 1.8-fold and 3.4-fold higher in ERG6 and 3 6 ERG11 deletion strains, respectively, than BY4742. Squalene was also found to be higher at the 3 7 optimized temperature of 30°C and pH 6.0. Furthermore, tolerance of S. cerevisiae to external 3 8 squalene at various concentration has been carried and found that the organism was tolerant up to 3 9 25 g/L of squalene. 0Conclusions: Homogenization based mechanical disruption was observed to yield higher 4 1 squalene and the SEM analysis corroborates these findings. The synergistic effect of ERG11 4 2 downregulation and tHMG1 over-expression has led to significant increase in squalene yield. 4 3 Significance and Impact of the Study: Sonication and homogenization has been used as a cell-4 4 disruption method for the first time in squalene extraction and squalene yield from 4 5homogenization method of cell lysis in BY4742 has been found to be 9 mg/g DCW which is the 4 6 highest reported so far in a wild-type strain. 4 7

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“…In recent years the microbial biosynthesis of squalene became a promising alternative source. Although the microorganisms don't accumulate as much squalene as shark liver or some plants, they grow very fast and in controlled conditions [8][9][10][11][12][13][14][15][16].…”

mentioning

confidence: 99%

Optimisation of Squalene Recovery from Amaranth Oil by Short Path Distillation

Băbeanu¹,

Sultana²,

Popa³

et al. 2018

Rev. Chim.

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We aim to determine the optimal conditions for obtaining a higher concentration of squalene (C30H50) with an increased yield from oil extracted from Amaranthus cruentus cultivated in Romania. A central composite experiment design was carried out to study the effect of operating conditions on the squalene concentration and recovery yield using short path distillation at laboratory scale. Among the three variables studied: feed flow rate, evaporator temperature and wiper speed, the most important proved to be evaporator temperature and the flow rate. Using the proposed models, we have identified three sets of values for the mentioned parameters, which ensure either a maximum squalene concentration or the best value for the squalene recovery yield, or an optimum between the maximum concentration and the best yield.

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Cited by 56 publications

References 12 publications

Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities

Engineering Strategies in Microorganisms for the Enhanced Production of Squalene: Advances, Challenges and Opportunities

Studies on squalene biosynthesis and the standardization of its extraction methodology fromSaccharomyces cerevisiae

Optimisation of Squalene Recovery from Amaranth Oil by Short Path Distillation

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