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Campos-Olivas, Ramón; Newman, J.; Ndassa, Yasmine; Summers, Michael F.

doi:10.1023/a:1008327130654

Cited by 5 publications

(2 citation statements)

References 6 publications

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“…The fully matured RSV and HIV proteins are highly similar alpha-helical proteins, consisting of an N-terminal domain (NTD) and a C-terminal domain (CTD) separated by a short linker. A short unstructured region follows the last helix in the CTD (26)(27)(28)(29)(30)(31)(32).…”

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confidence: 99%

Potential Role for CA-SP in Nucleating Retroviral Capsid Maturation

England

Purdy

Ropson

et al. 2014

J Virol

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confidence: 99%

Potential Role for CA-SP in Nucleating Retroviral Capsid Maturation

England

Purdy

Ropson

et al. 2014

J Virol

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“…Although NMR spectroscopy is commonly applied in vitro to screen compound (and especially fragment) libraries against certain targets, [12] to the best of our knowledge NMR spectroscopy has not been applied to date for the screening of endogenous enzymes in whole cell samples.T he setup is straightforward and robust as long as as pecific enzymatic reaction or target binding is monitored and the solubility of the probed substrate is over 50 mm.I nc ontrast to other biophysical methods such as surface plasmon resonance and mass spectrometry,n oi mmobilization of cells or post-screen cell lysis are required. It is cost-effective and time-efficient as protein over-expression and purification steps are avoided.…”

Section: Methodsmentioning

confidence: 99%

Target‐Based Whole‐Cell Screening by ¹H NMR Spectroscopy

Cao

McLeod

et al. 2015

Angewandte Chemie

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An NMR-based approachm arries the two traditional screening technologies (phenotypic and target-based screening) to find compounds inhibiting as pecific enzymatic reaction in bacterial cells.B uilding on ap revious study in which it was demonstrated that hydrolytic decomposition of meropenem in living Escherichia coli cells carrying New Delhi metallo-b-lactamase subclass 1(NDM-1) can be monitored in real time by NMR spectroscopy, we designed acell-based NMR screening platform. As trong NDM-1 inhibitor was identified with cellular IC 50 of 0.51 mm,w hich is over 300-fold more potent than captopril, ak nownN DM-1 inhibitor.T his new screening approach has great potential to be applied to targets in other cell types,such as mammalian cells,and to targets that are only stable or functionally competent in the cellular environment.

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