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Abstract

A spectroscopic method called Raman spectroscopy is frequently employed to discover the vibrational modes of molecules. Raman spectroscopy is frequently used in chemistry to give compounds a unique structural fingerprint. Using mass spectroscopy, one or more molecules present in a sample can have their mass to charge ratio (m/z) measured. It is frequently possible to estimate the precise molecular weight of the sample's constituent parts using these measures. Raman spectroscopy and mass spectroscopy are complimentary analytical methods that are used to offer details on the chemical make-up and functional groups of the target analytes. Each device offers particular chemical data. When the raman spectroscopy is hyphenated with mass spectroscopy the interface used is a small piece of tissue paper. Biological Sample Analysis, New medication chemical analysis, to distinguish between positional isomers. The hyphenated approach makes it simple to get analytical findings such as molecular weights, chemical structures, and functional groups. INTRODUCTION:Raman Spectroscopy is a molecular spectroscopic method for studying the properties of materials by taking use of the interaction between light and matter 1, 2 . Raman spectroscopy uses light scattering to produce the data it collects. Raman spectroscopy may be used to identify compounds since it produces a spectrum that is representative of the precise vibrations of a molecule (molecular fingerprinting) 3, 4 . In the analytical process of mass spectroscopy, chemical compounds are recognized by classifying gaseous ions in magnetic and electric fields according to their mass to charge ratio (m/z) 5, 6 .