Untitled

Abstract

The quality of Artemia cysts size can be determined by biometrical characterization. In this study, the combined effects of salinity and ferric citrate were studied on the biometric characterization of Artemia by single clonal reproduction under the laboratory condition. Results of control treatment showed increasing of the salinity correlates non-significant with diameter of decapsulated cyst and negatively correlates with the size of untreated cyst and chorion thickness. The effect of low concentrations of ferric citrate (5 and 10 mg/L) led to release the largest cysts in low salinity (35 ppt), while in high concentrations of ferric (20 and 40 mg/L) the largest cyst was released in medium salinity (70 ppt).

Effect of Different Culture Conditions on Gene Expression Associated With Cyst Production in Populations of Artemia franciscana

“…In this work, we provide evidence on the significant effect of salinity and iron concentration on the chorion thickness in two A. franciscana populations. Our results coincide with Wang et al (2019) , given that they found that increased salinity had a negative effect on the chorion thickness. Moreover, since these authors reported that variation in iron concentration also had an effect on the chorion thickness, which was thinner at 5 mg of Fe 2+ /L and thicker at higher iron concentrations, our result also concurs with these findings.…”

“…This result is not rare given that available data in Artemia indicate that there is wide interpopulation variation in the chorion thickness ( Sorgeloos et al, 1986 ). There is little information on factors that affect the chorion thickness in Artemia ( Wang et al, 2019 ). In this work, we provide evidence on the significant effect of salinity and iron concentration on the chorion thickness in two A. franciscana populations.…”

“…In addition, we determined the diameter of hydrated (DHC) or decapsulated (DDC) cysts (µm), together with chorion thickness (CT). This parameter was analyzed due to it varying as a function of salinity, iron concentration, and diet availability ( Wang et al, 2019 ), and therefore, its analysis may have an applied value. Decapsulation of the cysts was performed according to the Sorgeloos et al (1986) protocol.…”

“…From a reproductive point of view, this organism is characterized by two reproductive modes, oviparous and ovoviviparous, enabling it to adapt to these extreme environments. The first breeding mode is a diapause process driven by environmental factors related to variations in salinity, temperature, photoperiod, iron concentration, and diet ( Versichele and Sorgeloos, 1980 ; Wang et al, 2017 , Wang et al, 2019 ). The result is the production of a cyst that contains a gastrulae embryo in the diapause stage and is coated with a rigid chitinous shell ( Jackson and Clegg, 1996 ; Liang and MacRae, 1999 ; MacRae 2003 ).…”