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Křivánková, Ludmila; Ocko, Michal; Popelínský, Lubomír; Boček, Petr

doi:10.1002/1522-2683(200210)23:19<3364::aid-elps3364>3.0.co;2-r

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Capillary zone electrophoresis (CZE) is a powerful separation tool for small particles based on electrification property and particle weight . It would be a good choice for separating exosomes from different sources with CZE, as they may have different electrification property and particle weight.…”

Section: Methodsmentioning

confidence: 99%

A preliminary origin‐tracking study of different densities urinary exosomes

Lan

Qiao

et al. 2018

Electrophoresis

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Based on density differences of different subpopulations of exosomes, two kinds of micro‐vesicles with different densities were captured from urine by a modified sucrose density gradient ultracentrifuge separation method. Verified by transmission electron microscope (TEM) and western blot, the results showed these two kinds of micro‐vesicles were all exosomes. And these two kinds of exosomes were analyzed by TEM, 2D electrophoresis (2DE), and capillary zone electrophoresis (CZE), respectively. The results of TEM showed these two exosomes with different densities have different morphological characteristics, and some tiny proteomic differences were shown in the results of 2DE of these two exosomes. At the same time, the CZE results displayed these two kinds of exosomes possessed different retention times, indicated that they may have different electrification property and particle weight. These results may attribute to their different origins. This work may provide a preliminary experience for the origin‐tracking study for urinary exosomes, and would be more useful for future targeted biomarker discovery.

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Section: Methodsmentioning

confidence: 99%

A preliminary origin‐tracking study of different densities urinary exosomes

Lan

Qiao

et al. 2018

Electrophoresis

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“…When there is a flow, the direction of flow is essentially parallel to the plane of the membranes and orthogonal to the direction of the electric field 1–4. Though IET was conceived for the semi‐preparative scale separation of proteins, as reviewed in 8–10, it has morphed into a valued prefractionation technique in proteomics (for recent reviews see 11, 12; for the first original papers, see 13–17).…”

Section: Introductionmentioning

confidence: 99%

Simulation of desalting that occurs during isoelectric trapping separations

2009

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Simul 5, the simulation program based on the 1-D model of electrophoresis has been extended to simulate isoelectric trapping (IET) separations that take place in recirculating multicompartmental electrolyzers (MCEs). In the extended Simul 5, the simulated separation space between the anode and cathode can be divided into multiple segments to represent the anode compartment, separation compartment(s) and the cathode compartment. The compartments may have identical or different cross sections. A new algorithm simulates convective mixing that occurs in the recirculating MCEs where the distances between the buffering membranes are short and the velocities of tangential flows through the compartments, orthogonal to the electric field, are high. The intensity of simulated mixing can be independently controlled in each compartment. pH transients that were reported to occur during the desalting step in IET separations were simulated with the extended Simul 5 program: the main features of the experimental results were reproduced by the simulations. The simulations shed light on the possible causes of uneven anion and cation removal rates, pH transients and the transient invasion of the separation compartment by components of the electrode solutions that might occur during the desalting step.

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Fast and simple method for determination of iodide in human urine, serum, sea water, and cooking salt by capillary zone electrophoresis

Pantůčková

Křivánková

2004

Electrophoresis

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For the determination of iodide in urine, where 80-90% of consumed iodine is excreted, a fast, simple, and sensitive method of capillary zone electrophoresis was elaborated and tested also for additional complex matrices such as human serum, cooking salt, and seawater. Several approaches were examined for the separation of iodide from other macro- and microcomponents in the tested matrices, and the best results were obtained when host-guest interaction with alpha-cyclodextrin or ion-pairing with polyethylenimine was employed. In both cases comparable resolution and sensitivity were reached. Due to the relatively high price of cyclodextrin only the method with polyethylenimine was further optimized and a simple procedure enabling the determination of iodide in untreated human urine, serum, cooking salt, and seawater was elaborated. The samples were injected for 20 s at 0.5 psi (3.45 kPa) into a fused-silica capillary (0.18 mm ID, 50 cm effective length) coated with polyacrylamide (electroosmotic flow < 2 x 10(-9) m(2)V(-1)s(-1)) and filled with the optimized background electrolyte composed of 20 mM KH(2)PO(4) and 0.7% m/v polyethylenimine. For detection, UV absorption at 200 and 230 nm was measured. Concentration limits of detection reached at 230 nm were for human urine 0.14 microM, for human serum 0.17 microM, for seawater 0.17 microM, and for cooking salt 89 nM. Relative standard deviations of iodide peak area and height in all matrices ranged within 0.93 to 4.19%.

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Untitled

Cited by 3 publications

References 6 publications

A preliminary origin‐tracking study of different densities urinary exosomes

A preliminary origin‐tracking study of different densities urinary exosomes

Simulation of desalting that occurs during isoelectric trapping separations

Fast and simple method for determination of iodide in human urine, serum, sea water, and cooking salt by capillary zone electrophoresis

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