R11 Peptides Can Promote the Molecular Imaging of Spherical Nucleic Acids for the Bladder Cancer Margin Identification

Ma, Minghai; Zhang, Pu; Liang, Xiao; Cui, Daxiang; Shao, Qiuya; Zhang, Mengzhao; Yang, Tao; Wang, Lu; Zhang, Nan; Jing, Minxuan; Zhang, Lu; Dan, Weichao; Song, Rundong; Liu, Xi; Hao, Jiatao; Chen, Yuhang; Gu, Lijiang; Wang, Lei; Fan, Jinhai

doi:10.2139/ssrn.3825979

Cited by 2 publications

(6 citation statements)

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“…Previously, we adopted the hendeca-arginine peptide (R11) to generate nanoprobes to perform molecular imaging of spherical nucleic acids, leading to effective identification of bladder cancer margins and a significant improvement in the R0 resection rate in surgery. 9 We also observed that R11 peptides have the potential to significantly increase the BCa targeting ability and membrane penetrability of nucleic acid materials during bioimaging. 9 Because R11 is positively charged, it can interact with the negatively charged phosphate backbone of nucleic acids (including RNA and DNA) through electrostatic interactions to self-assemble into nanocomplexes.…”

Section: Introductionmentioning

confidence: 60%

“… 9 We also observed that R11 peptides have the potential to significantly increase the BCa targeting ability and membrane penetrability of nucleic acid materials during bioimaging. 9 Because R11 is positively charged, it can interact with the negatively charged phosphate backbone of nucleic acids (including RNA and DNA) through electrostatic interactions to self-assemble into nanocomplexes. 10 This makes R11 a potential carrier for cancer gene therapy.…”

Section: Introductionmentioning

confidence: 60%

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Combined Self-Assembled Hendeca-Arginine Nanocarriers for Effective Targeted Gene Delivery to Bladder Cancer

Zhang¹,

Zheng²,

Wang³

et al. 2022

IJN

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Introduction Bladder cancer (BCa) is among the most prevalent cancers worldwide. However, the effectiveness of intravesical therapy for BCa is limited due to the short dwell time and the presence of the permeation barrier. Methods Nanocomplexes were self-assembled between DNA and hendeca-arginine peptide (R11). Stepwise intravesical instillation of R11 and the generated nanocomplexes significantly enhanced the targeting capacity and penetration efficiency in BCa therapy. The involved mechanism of cellular uptake and penetration of the nanocomplexes was determined. The therapeutic effect of the nanocomplexes was verified preclinically in murine orthotopic BCa models. Results Nanocomplexes exhibited the best BCa targeting efficiency at a nitrogen-to-phosphate (NP) ratio of 5 but showed a lack of stability during cellular uptake. The method of stepwise intravesical instillation not only increased the stability and target specificity of the DNA component but also caused the delivered DNA to more effectively penetrate into the glycosaminoglycan layer and plasma membrane. The method promotes the accumulation of the delivered DNA in the clathrin-independent endocytosis pathway, directs the intracellular trafficking of the delivered DNA to nonlysosome-localized regions, and enables the intercellular transport of the delivered DNA via a direct transfer mechanism. In preclinical trials, our stepwise method was shown to remarkably enhance the targeting and penetration efficiency of DNA in murine orthotopic BCa models. Conclusion With this method, a stepwise intravesical instillation of self-assembled nanocomplexes, which are generated from hendeca-arginine peptides, was achieved; thus, this method offers an effective strategy to deliver DNA to target and penetrate BCa cells during gene therapy and warrants further development for future intravesical gene therapy in the clinical context.

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Section: Introductionmentioning

confidence: 60%

Section: Introductionmentioning

confidence: 60%

Combined Self-Assembled Hendeca-Arginine Nanocarriers for Effective Targeted Gene Delivery to Bladder Cancer

Zhang¹,

Zheng²,

Wang³

et al. 2022

IJN

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“…For instance, survivin , a member of the apoptosis inhibitory protein family, can serve as a specific marker for BC [63] . For sensitive detection of BC margins in surgery, nanoprobes (AuNP-MB@R11) containing molecular beacon (MB) corresponding to survivin and R11 peptide-linked spherical nucleic acids (SNAs) were synthesized [64] . Researchers first constructed SNAs composed of AuNPs and MB to quantify survivin mRNA [65] .…”

Section: Nanomaterials For Improving Recognition Specificity and Kill...mentioning

confidence: 99%

“…A cancerous fluorescence margin is observed. Thus, AuNP-MB@R11 can be regarded as an intraoperative molecular imaging technique to achieve complete tumor resection and improve recurrence-free survival [64] . In addition, chitosan-based nanomedicine that delivers gambogic acid prodrug in response to GSH and ROS signaling has also been reported [66] .…”

Section: Nanomaterials For Improving Recognition Specificity and Kill...mentioning

confidence: 99%

“…[ 63 ] For sensitive detection of BC margins in surgery, nanoprobes (AuNP-MB@R11) containing molecular beacon (MB) corresponding to survivin and R11 peptide-linked spherical nucleic acids (SNAs) were synthesized. [ 64 ] Researchers first constructed SNAs composed of AuNPs and MB to quantify survivin mRNA. [ 65 ] Then, due to the super BC targeting and cellular uptake potency of the poly (11)-arginine cell-penetrating peptide (R11), they further attached R11 peptides to SNAs to form AuNP-MB@R11 NPs.…”

Section: Nanomaterials For Improving Recognition Specificity and Kill...mentioning

confidence: 99%

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Application of synthetic biology in bladder cancer

Ding

Lin

Qin

et al. 2022

Chinese Medical Journal

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Bladder cancer (BC) is the most common malignant tumor of the genitourinary system. The age of individuals diagnosed with BC tends to decrease in recent years. A variety of standard therapeutic options are available for the clinical management of BC, but limitations exist. It is difficult to surgically eliminate small lesions, while radiation and chemotherapy damage normal tissues, leading to severe side effects. Therefore, new approaches are required to improve the efficacy and specificity of BC treatment. Synthetic biology is a field emerging in the last decade that refers to biological elements, devices, and materials that are artificially synthesized according to users’ needs. In this review, we discuss how to utilize genetic elements to regulate BC-related gene expression periodically and quantitatively to inhibit the initiation and progression of BC. In addition, the design and construction of gene circuits to distinguish cancer cells from normal cells to kill the former but spare the latter are elaborated. Then, we introduce the development of genetically modified T cells for targeted attacks on BC. Finally, synthetic nanomaterials specializing in detecting and killing BC cells are detailed. This review aims to describe the innovative details of the clinical diagnosis and treatment of BC from the perspective of synthetic biology.

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R11 Peptides Can Promote the Molecular Imaging of Spherical Nucleic Acids for the Bladder Cancer Margin Identification

Cited by 2 publications

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Combined Self-Assembled Hendeca-Arginine Nanocarriers for Effective Targeted Gene Delivery to Bladder Cancer

Combined Self-Assembled Hendeca-Arginine Nanocarriers for Effective Targeted Gene Delivery to Bladder Cancer

Application of synthetic biology in bladder cancer

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