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Ugwu, Stanley Ifeanyi; Kowalska, Agata; Morita, Masaya; Kowalski, Radosław Kajetan

doi:10.4194/1303-2712-v19_1_05

Cited by 2 publications

(2 citation statements)

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“…Generally, methanol was effective in cryopreservation of sperm from freshwater fish, including zebrafish, Danio rerio [ 30 ]; cyprinid fish, Neolissochilus soroides (Valenciennes, 1842) [ 31 ]; black-stripe black crappie, Pomoxis nigromaculatus [ 32 ]; goldfish, Carassius auratus [ 33 ]; Japanese bitterling [ 34 ]; and masu salmon, Oncorhynchus masou [ 35 ]. In contrast, glucose-methanol has been employed for the cryopreservation of sperm from Mozambique tilapia, Oreochromis mossambicus [ 36 ]. Moreover, glycerol was effective for the cryopreservation of sperm from several species, including giant grouper [ 9 ], pointhead flounder ( Cleisthenes pinetorum herzensteini ) [ 37 ], and longtooth grouper ( Epinephelus bruneus ) [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Roughscale Sole (Clidoderma asperrimum) Sperm: Effects of Cryoprotectant, Diluent, Dilution Ratio, and Thawing Temperature

Zidni

Lee

Yoon

et al. 2022

Animals

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The roughscale sole, Clidoderma asperrimum is categorized as an endangered species. Sperm freezing is essential for preserving gametes. This study examined the CPA concentration, diluent, dilution ratio, and thawing temperature to design a sperm cryopreservation protocol for roughscale sole. The variables examined included sperm motility and kinematics, cell survival, fertilization, and DNA fragmentation. Sperm motility parameters were assessed via computer-assisted sperm analysis using a CEROS II instrument. Cell survival rate and DNA damage were assessed using the Cell Counting Kit-8 and single-cell gel electrophoresis assay, respectively. Sperm preservation was tested using several CPAs, including ethylene glycol, dimethyl sulfoxide (DMSO), glycerol, propylene glycol, and methanol. The diluents tested were 300 mM sucrose, 300 mM glucose, Stein’s solution, Ringer’s solution, and Hank’s solution. The optimal conditions for sperm cryopreservation were 10% DMSO + Stein’s solution. After thawing, sperm motility was highest with a 1:1 dilution ratio (sperm to CPA + diluent), at 69.20 ± 0.32%; thawing at 10 °C was optimal for post-thaw motility (72.03 ± 0.95%). The highest fertilization rate (40.00 ± 1.22%) was obtained using DMSO. The fresh sperm had the lowest tail DNA, followed by 10% DMSO + Stein’s solution. The developed cryopreservation methods can be used in roughscale sole hatcheries.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation of Roughscale Sole (Clidoderma asperrimum) Sperm: Effects of Cryoprotectant, Diluent, Dilution Ratio, and Thawing Temperature

Zidni

Lee

Yoon

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…The fish sperm of many species have been cryopreserved successfully e.g. Eurasian perch, Perca fluviatilis (Bernáth et al, 2016); carp, Cyprinus carpio (Boryshpolets et al, 2017); Atlantic salmon, Salmo salar (Figueroa et al, 2018); zebra fish, Dana rerio (Matthews et al, 2018;Rebocho, 2018); Mozambique tilapia, Oreochromis mossambicus (Peters, 1852▬ Ugwu et al, 2018; rainbow trout Oncorhynchus mykiss (Walbaum, 1792▬ Ciereszko et al, 2014Kutluyer et al, 2014); bagrid catfish, Mystus nemurus (Valenciennes, 1840 ▬ Muchlisin andSiti-Azizah, 2009); African catfish, Clarias gariepinus Burchell, 1822 (Muchlisin et al, 2015;Olanrewaju et J o u r n a l P r e -p r o o f al., 2015); giant gourami, Osphronemus goramy Lacépède, 1801 (Abinawanto et al, 2017); Java barb, Barbonymus gonionotus (Bleeker, 1850▬ Abinawanto et al, 2016, and sharkminnow Osteochilus vittatus (Valenciennes, 1842▬ Muthmainnah et al, 2018. However, the preservation of God's fish sperm has not been reported yet.…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 99%