R.SspD5I is a neoschizomer ofHphI producing blunt end DNA fragments

Abstract

The strain Staphylococcus species D5 produces a restriction enzyme. It is the neoschizomer of HphI endonuclease, which cleaves DNA at a distance of eight nucleotides from the recognition sequences producing blunt end DNA fragments: 5'-GGTGA8N /-3' and 3'-CCACT8N /-5'.

Location of the Bases Modified by M.BcoKIA and M.BcoKIB Methylases in the Sequence 5′-CTCTTC-3′/5′-GAAGAG-3′

“…The methyltransferases M.BcoKIA and M.BcoKIB and cognate restriction endonuclease BcoKI, an isoschizomer of Ksp632I [10], recognize the asymmetric site 5′ CTCTTC 3′/5′ GAAGAG 3′ and R.BcoKI cuts DNA at a distance of one and four nucleotides distal to the sequence. There are three cytosines in the top strand and three adenines in the bottom strand of the recogni tion sequence.…”