R.I.A. Kit for Assay of Hepatitis-B Antigen

Leers, W. D.; Kouroupis, G. M.

doi:10.1016/s0140-6736(73)92917-6

“…This fault could be corrected satisfactorily by absorbing the sera with anti-HB-Ab and retesting (7). Modification of the Ausria-I failed to solve the problem of specificity satisfactorily in our hands (9).…”

Section: Discussionmentioning

confidence: 71%

Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen

Leers¹,

Kouroupis²

1975

J Clin Microbiol

3

0

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Radioimmunoassay for the detection of hepatitis B antigen has been accepted in many diagnostic laboratories now. The question of nonspecific positives has always been a matter of controversy. Two improved radioimmunoassay tests, namely Ausria II-125 by Abbott Laboratories and a radioimmunoassay method by Connaught Laboratories Limited (Hebria), were compared with the original Ausria 125I. Included in the comparison was the reversed passive hemagglutination test (Auscell, Abbott). Five hundred sera of clinical patients were tested. Fifty-five or 11% were found to have hepatitis B antigen. Three tests, Ausria 125I, Ausria II-125, and Hebria showed the same number of positive sera, whereas Auscell missed one. However, Ausria 125I indicated two additional false positives. Dilution experiments, however, indicated that Ausria II-125 and Hebria were the most sensitive tests, with the reversed passive hemaglutination showing the least sensitivity. Therefore, the new Ausria II-125 and the Hebria radioimmunoassay tests are preferable in view of their sensitivity and specificity.

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“…Abbott Laboratories has recently modified the RIA kit by adding guinea pig serum to the radioactive labeled anti-HB. (6). It is hoped that by this step the problem with nonspecific reactivity to guinea pig protein will be overcome.…”

Section: Discussionmentioning

confidence: 99%

Specificity and Sensitivity of Radioimmunoassay for Hepatitis B Antigen

Irwin¹,

Allen²,

Bancroft³

et al. 1974

Applied Microbiology

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Sera from a survey of 6,026 people were tested for hepatitis B surface antigen by using radioimmunoassay and counterelectrophoresis. Forty-eight sera (0.79%) were positive by counterelectrophoresis and 152 sera (2.52%) were positive by radioimmunoassay, using the most liberal of the recommended criteria for positivity (i.e., counts 3 standard deviations above the mean). Absorption tests performed on the 152 radioimmunoassay-positive sera showed that 10 (6.6%) were false-positive reactions to guinea pig protein, 74 (48.6%) were due to false-positive reaction(s) with other protein(s) in the test system, and 68 (44.8%) were true positives. There was a strong correlation between the degree of elevation of radioactive counts and the proportions of sera that were true positives; all 49 sera with counts >50 standard deviation units above the mean were true positives, but only 19 (18.4%) of the 103 sera with counts <50 standard deviation units were true positives. A few sera with high counts required absorption with type-specific (type D) antisera. The following conclusions were reached from this study: (i) absorption tests should be run on all radioimmunoassay-positive, counterelectrophoresis-negative sera; (ii) most (about 90%) false positives are not due to anti-guinea-pig protein reactions; and (iii) radioimmunoassay, in combination with absorption tests, yields a modest increase (about 35%) in detection of true positives over use of counterelectrophoresis alone.

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Specificity and Sensitivity of Radioimmunoassay for Hepatitis B Antigen

Irwin

¹

,

Allen

²

,

Bancroft

³

et al. 1974

Appl Microbiol

7

0

View full text Add to dashboard Cite

Sera from a survey of 6,026 people were tested for hepatitis B surface antigen by using radioimmunoassay and counterelectrophoresis. Forty-eight sera (0.79%) were positive by counterelectrophoresis and 152 sera (2.52%) were positive by radioimmunoassay, using the most liberal of the recommended criteria for positivity (i.e., counts 3 standard deviations above the mean). Absorption tests performed on the 152 radioimmunoassay-positive sera showed that 10 (6.6%) were false-positive reactions to guinea pig protein, 74 (48.6%) were due to false-positive reaction(s) with other protein(s) in the test system, and 68 (44.8%) were true positives. There was a strong correlation between the degree of elevation of radioactive counts and the proportions of sera that were true positives; all 49 sera with counts >50 standard deviation units above the mean were true positives, but only 19 (18.4%) of the 103 sera with counts <50 standard deviation units were true positives. A few sera with high counts required absorption with type-specific (type D) antisera. The following conclusions were reached from this study: (i) absorption tests should be run on all radioimmunoassay-positive, counterelectrophoresis-negative sera; (ii) most (about 90%) false positives are not due to anti-guinea-pig protein reactions; and (iii) radioimmunoassay, in combination with absorption tests, yields a modest increase (about 35%) in detection of true positives over use of counterelectrophoresis alone.

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R.I.A. Kit for Assay of Hepatitis-B Antigen

Cited by 3 publications

References 2 publications

Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen

Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen

Specificity and Sensitivity of Radioimmunoassay for Hepatitis B Antigen

Specificity and Sensitivity of Radioimmunoassay for Hepatitis B Antigen

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