Quorum Signal Inhibitors and Their Potential Use against Fish Diseases

Chu, Weihua; McLean, Robert

doi:10.1080/08997659.2016.1150907

Cited by 12 publications

(7 citation statements)

References 46 publications

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“…In addition, uptake of substances such as propidium iodide or N -phenyl-l-napthylamine indicates that formation of unregulated pores in the membrane and an increased probability of cell death. Finally, cell-to-cell communication among bacteria (quorum sensing), used to control group behaviors, including virulence factor production or biofilm formation, is another central target that could help to reduce antimicrobial resistance [ 46 ].…”

Section: Mechanisms Of Essential Oil Cytotoxicitymentioning

confidence: 99%

Biological Activities of Essential Oils: From Plant Chemoecology to Traditional Healing Systems

et al. 2017

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Essential oils are complex mixtures of hydrocarbons and their oxygenated derivatives arising from two different isoprenoid pathways. Essential oils are produced by glandular trichomes and other secretory structures, specialized secretory tissues mainly diffused onto the surface of plant organs, particularly flowers and leaves, thus exerting a pivotal ecological role in plant. In addition, essential oils have been used, since ancient times, in many different traditional healing systems all over the world, because of their biological activities. Many preclinical studies have documented antimicrobial, antioxidant, anti-inflammatory and anticancer activities of essential oils in a number of cell and animal models, also elucidating their mechanism of action and pharmacological targets, though the paucity of in human studies limits the potential of essential oils as effective and safe phytotherapeutic agents. More well-designed clinical trials are needed in order to ascertain the real efficacy and safety of these plant products.

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Section: Mechanisms Of Essential Oil Cytotoxicitymentioning

confidence: 99%

Biological Activities of Essential Oils: From Plant Chemoecology to Traditional Healing Systems

et al. 2017

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“…luxS is responsible for the production of AI-2 QS signal molecules, commonly referred to as AI-2 molecules [ 57 ]. As previous studies revealed that the luxS isogenic mutant of A. hydrophila altered biofilm formation dynamics and its architecture, bacterial motility reduced and virulence has been enhanced observed in a mouse model [ 58 ] and luxS mutant strain failed to produce AI-2 as studied in Aeromonas salmonicida [ 59 ] Another report shows that tannic acid is also a potential QS inhibitor, which reduces biofilm formation, swarming motility and blood haemolysis activity of fish pathogenic bacteria [ 60 , 61 ]. Moreover, 5906 a small peptide binds with Luxs and prevents the synthesis of functional LuxS homodimer of Edwersilla tarada [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

In-Silico Prediction and Modeling of the Quorum Sensing LuxS Protein and Inhibition of AI-2 Biosynthesis in Aeromonas hydrophila

Ali

Yao

et al. 2018

Molecules

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luxS is conserved in several bacterial species, including A. hydrophila, which causes infections in prawn, fish, and shrimp, and is consequently a great risk to the aquaculture industry and public health. luxS plays a critical role in the biosynthesis of the autoinducer-2 (AI-2), which performs wide-ranging functions in bacterial communication, and especially in quorum sensing (QS). The prediction of a 3D structure of the QS-associated LuxS protein is thus essential to better understand and control A. hydrophila pathogenecity. Here, we predicted the structure of A. hydrophila LuxS and characterized it structurally and functionally with in silico methods. The predicted structure of LuxS provides a framework to develop more complete structural and functional insights and will aid the mitigation of A. hydrophila infection, and the development of novel drugs to control infections. In addition to modeling, the suitable inhibitor was identified by high through put screening (HTS) against drug like subset of ZINC database and inhibitor ((−)-Dimethyl 2,3-O-isopropylidene-l-tartrate) molecule was selected based on the best drug score. Molecular docking studies were performed to find out the best binding affinity between LuxS homologous or predicted model of LuxS protein for the ligand selection. Remarkably, this inhibitor molecule establishes agreeable interfaces with amino acid residues LYS 23, VAL 35, ILE76, and SER 90, which are found to play an essential role in inhibition mechanism. These predictions were suggesting that the proposed inhibitor molecule may be considered as drug candidates against AI-2 biosynthesis of A. hydrophila. Therefore, (−)-Dimethyl 2,3-O-isopropylidene-l-tartrate inhibitor molecule was studied to confirm its potency of AI-2 biosynthesis inhibition. The results shows that the inhibitor molecule had a better efficacy in AI-2 inhibition at 40 μM concentration, which was further validated using Western blotting at a protein expression level. The AI-2 bioluminescence assay showed that the decreased amount of AI-2 biosynthesis and downregulation of LuxS protein play an important role in the AI-2 inhibition. Lastly, these experiments were conducted with the supplementation of antibiotics via cocktail therapy of AI-2 inhibitor plus OXY antibiotics, in order to determine the possibility of novel cocktail drug treatments of A. hydrophila infection.

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“…Quorum quenching is the process of disrupting quorum sensing, that is a mode of social life communication among microbes dependent on cell density that regulates a plethora of behaviours, including virulence in many aquatic pathogens such as Aeromonas , Vibrio and Edwardsiella (Chu & McLean, 2016). Quorum sensing inhibitors are synthetic or natural molecules that interfere and disrupt this communication mechanism.…”

Section: Changes In the Selective Context Along The Production Chainmentioning

confidence: 99%

“…Given the complexity of live cultures, an alternative option is to directly use the molecular compounds. Some authors advocate the search for these invariant microbiota‐harnessing tools as alternative antimicrobials, like quorum sensing inhibitors (Chu & McLean, 2016) and bacteriocins (Sahoo et al., 2016). In food, compounds that underlie spoilage are often under the control of quorum sensing mechanisms.…”

Section: Microbial Community Modulation Strategies From Egg To Fork: mentioning

confidence: 99%

A continuously changing selective context on microbial communities associated with fish, from egg to fork

Derôme

Filteau

2020

Evolutionary Applications

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Fast increase of fish aquaculture production to meet consumer demands is accompanied by important ecological concerns such as disease outbreaks. Meanwhile, food waste is an important concern with fish products since they are highly perishable. Recent aquaculture and fish product microbiology, and more recently, microbiota research, paved the way to a highly integrated approach to understand complex relationships between host fish, product and their associated microbial communities at health/disease and preservation/spoilage frontiers. Microbial manipulation strategies are increasingly validated as promising tools either to replace or to complement traditional veterinary and preservation methods. In this review, we consider evolutionary forces driving fish microbiota assembly, in particular the changes in the selective context along the production chain. We summarize the current knowledge concerning factors governing assembly and dynamics of fish hosts and food microbial communities. Then, we discuss the current microbial community manipulation strategies from an evolutionary standpoint to provide a perspective on the potential for risks, conflict and opportunities. Finally, we conclude that to harness evolutionary forces in the development of sustainable microbiota manipulation applications in the fish industry, an integrated knowledge of the controlling abiotic and especially biotic factors is required.

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Quorum Signal Inhibitors and Their Potential Use against Fish Diseases

Cited by 12 publications

References 46 publications

Biological Activities of Essential Oils: From Plant Chemoecology to Traditional Healing Systems

Biological Activities of Essential Oils: From Plant Chemoecology to Traditional Healing Systems

In-Silico Prediction and Modeling of the Quorum Sensing LuxS Protein and Inhibition of AI-2 Biosynthesis in Aeromonas hydrophila

A continuously changing selective context on microbial communities associated with fish, from egg to fork

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