Quorum sensing systems, related virulence factors, and biofilm formation in Pseudomonas aeruginosa isolated from fish

Sırıken, Belgin; Öz, Veli; Erol, İrfan

doi:10.1007/s00203-020-02159-5

Cited by 10 publications

(4 citation statements)

References 54 publications

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“…The LuxI enzyme produces the QS signal molecule (the N-3-oxo-hexanoyl-homoserine lactone), and LuxR is a transcription regulator that binds to the AHL signal and regulates bioluminescence via the luxCDABEG operon [ 9 ]. Following the discovery of the AHL-QS system, researchers discovered that AHL-QS is prevalent in a wide range of Proteobacteria and influences vital characteristics such as motility [ 10 ], pigment production [ 11 ], and biofilm formation [ 12 ]. Our molecular understanding of the QS system has been useful in studying plant–microbe, animal–microbe, and human–microbe interactions.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

et al. 2022

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Microbial biodiversity includes biotic and abiotic components that support all life forms by adapting to environmental conditions. Climate change, pollution, human activity, and natural calamities affect microbial biodiversity. Microbes have diverse growth conditions, physiology, and metabolism. Bacteria use signaling systems such as quorum sensing (QS) to regulate cellular interactions via small chemical signaling molecules which also help with adaptation under undesirable survival conditions. Proteobacteria use acyl-homoserine lactone (AHL) molecules as autoinducers to sense population density and modulate gene expression. The LuxI-type enzymes synthesize AHL molecules, while the LuxR-type proteins (AHL transcriptional regulators) bind to AHLs to regulate QS-dependent gene expression. Diverse AHLs have been identified, and the diversity extends to AHL synthases and AHL receptors. This review comprehensively explains the molecular diversity of AHL signaling components of Pseudomonas aeruginosa, Chromobacterium violaceum, Agrobacterium tumefaciens, and Escherichia coli. The regulatory mechanism of AHL signaling is also highlighted in this review, which adds to the current understanding of AHL signaling in Gram-negative bacteria. We summarize molecular diversity among well-studied QS systems and recent advances in the role of QS proteins in bacterial cellular signaling pathways. This review describes AHL-dependent QS details in bacteria that can be employed to understand their features, improve environmental adaptation, and develop broad biomolecule-based biotechnological applications.

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Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

et al. 2022

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“…32 There are many multifactorial dynamic processes that occur at various stages through well-established mechanisms leading to the colonization of the lungs and these steps are controlled by the QS and involve different virulence factors. 19,27 Adhesion is the initial stage of biofilm formation and this stage usually requires different structural compartments. 33 This makes use of flagella that ensure the swimming motility necessary for attachment, dissemination, colonization of the host epithelium and transition from the planktonic phase to the biofilm mode.…”

Section: Introductionmentioning

confidence: 99%

“…18 Elastase, associated with immune evasion (Las), rhamnolipid, related to the structure and dynamics of the biofilm (Rhl), Pseudomonas quinolone signal (Pqs) and integrated quorum sensing (Iqs). 8,19 The Las system has a dominant role in this hierarchy since it can interfere by positively controlling the expression of the other three systems. 1,20 The Las system controls the synthesis of N- (3-oxododecanoyl)-L- homoserine lactone (3-oxo C12-AHL) to activate the appropriate Las R regulator and also to trigger the expression of genes responsible for the production of Las A protease and Las B elastase as well as for the production of exotoxin-A.…”

Section: Introductionmentioning

confidence: 99%

Biofilm Disruption and Virulence Attenuation Effects of Essential Oil From Endemic Algerian Cistus munbyi (Cistaceae) Against Clinical Strains of Pseudomonas aeruginosa

Benaissa,

Khadir,

Tamfu

et al. 2024

Natural Product Communications

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Background: Pseudomonas aeruginosa is an opportunistic, resistant and highly pathogenic bacteria, responsible for severe nosocomial infections in humans. There is need to search new antimicrobials to target P. aeruginosa strains. Cistus munbyi is used in traditional medicine to manage infections. Objectives: This work aims to investigate the antimicrobial, antibiofilm and other antivirulence effects of essential oil from C. munbyi against P. aeruginosa. Materials and methods: Essential oil (EO) was prepared by hydro-distillation from C. munbyi, characterized by GC–MS and evaluated for its antimicrobial, antibiofilm and anti-virulence effects against clinical isolates of P. aeruginosa. Results: 100% of compounds were identified with Terpinen-4-ol (33.20%), Sabinene (13.20%), α-Thujene (11.30%), and p-Cymene (9.94%) being the major constituents. The EO had good antimicrobial activity determined by diameters of inhibition zones and broth dilution. At minimal inhibitory concentration (MIC), the EO eliminated more that 70% of young biofilms in 19 strains out of 24 tested strains while at 2MIC, only one strain had biofilm inhibition below 70%. The EO eliminated more than 50% of mature biofilms in 13 strains out of 24 tested. Biofilm disruption was concentration-dependent at sub-MIC and indicated good potential of C. munbyi EO as P. aeruginosa antibiofilm agent. Scanning electron microscopy (SEM) images revealed that untreated colonies had a well-developed biofilm while there was significant reduction of biofilms with distorted architecture and cell shrinkage upon treatment with EO. The C. munbyi EO at MIC and sub-MIC was able to reduce quorum-sensing (QS) virulence factors such as pyocyanin production, exopolysaccharides matrix and elastase secretion. Significant QS revealed by reduction of violacein in C. violaceum (CV12472 and CV026) was observed. Conclusion: The overall results show good anti-pseudomonal effects of C. munbyi EO which makes it a potential agent to reduce the spread of infections and resistance to antibiotics from P. aeruginosa strains.

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“…Each system (Las and Rhl) is encoded by two components: AI synthesis (lasI and rhlI genes) and their cognate transcriptional activating protein (lasR and rhlR genes). There is also a third system in P. aeruginosa known as the Pseudomonas quinolone signal, which is induced by 2-heptyl-3hydroxy-4-quinolone and controlled by the las and rhl systems (Brindhadevia et al, 2020;Sırıken et al, 2020;Elnegery et al, 2021). These systems in the QS mechanism cause the bacteria to have a successful role in pathogenicity by adjusting the population densities and activating the relevant virulence factors.…”

Section: Introductionmentioning

confidence: 99%

Quorum Sensing Mediated Pathogenicity, Virulence Genes and Class 1 Integron in Carbapenem-Resistant Clinical Pseudomonas Aeruginosa Isolates

Başkan

Sırıken

Tüfekci

et al. 2021

Preprint

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Carbapenems are the most effective agents for treating carbapenem-resistant clinical P. aeruginosa (CRPsA) infections. During an infection, a quorum-sensing (QS) system and its regulating virulence genes have a great role. The aim of the study was to detect the presence of a las and rhl QS system and related virulence genes and a class 1 (Cls1) integron. A total of 52 CRPsA isolates obtained from Kastamonu, Turkey was analyzed. A conventional culture method, an oprL gene-based molecular assay for P. aeruginosa isolates, and an automated VITEK-2 compact system were applied for the isolation and identification of CRPsA isolates. The oprL gene was detected in all of the isolates tested. At least one of the las or rhl system genes was detected in 98.07% of the isolates, and the percentage of las system genes (98.07%) were higher than that of rhl system genes (90.38%). algD, lasB, toxA and aprA genes were detected in between 46.15% and 88.46% of the isolates, and co-existence of four genes were detected in 40.38% of the isolates. Cls1 integron and slime production using Congo Red Agar (CRA) were present in 51.92% and 67.30%, respectively, of the isolates. There was a significant positive correlation (p <.10) between the las system and the rhl system and a strongly positive correlation (p<.01 or p <.05) between the rhl system-four virulence genes and slime production-and among some virulence genes. In conclusion, the CRPsA isolates tested in the study are highly virulent and QS systems have a significant role in pathogenesis.

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Quorum sensing systems, related virulence factors, and biofilm formation in Pseudomonas aeruginosa isolated from fish

Cited by 10 publications

References 54 publications

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

Molecular Mechanisms and Applications of N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Bacteria

Biofilm Disruption and Virulence Attenuation Effects of Essential Oil From Endemic Algerian Cistus munbyi (Cistaceae) Against Clinical Strains of Pseudomonas aeruginosa

Quorum Sensing Mediated Pathogenicity, Virulence Genes and Class 1 Integron in Carbapenem-Resistant Clinical Pseudomonas Aeruginosa Isolates

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