Quorum sensing signals affect spoilage of refrigerated large yellow croaker (Pseudosciaena crocea) by Shewanella baltica

Zhu, Junli; Zhao, Aifei; Feng, Lili; Gao, Haichun

doi:10.1016/j.ijfoodmicro.2015.10.020

Cited by 88 publications

(75 citation statements)

References 49 publications

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“…Then, 10-fold serial dilutions were made and were inoculated on IA (Hope Bio-Technology Company, Qingdao, China). After incubation at 25°C for 48 h, 43 bacteria were picked randomly and then the isolates were further purified on another IA plate at 25°C (Zhu et al 2016). Spoilage potential of those strains were preliminary investigated in the sterile fish juice stored at 4°C (results not shown), and the different spoilage capabilities of eight black colonies and two white colonies were taken and stored at À80°C in broth with 20% glycerol for further characterization.…”

Section: Methodsmentioning

confidence: 99%

Spoilage potential characterization ofShewanellaandPseudomonasisolated from spoiled large yellow croaker (Pseudosciaena crocea)

Zhu

et al. 2016

Lett Appl Microbiol

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Members of the bacterial genera Shewanella and Pseudomonas are widely known to be responsible for the specific spoilers in iced fish. Ten strains isolated from spoiled large yellow croaker (Pseudosciaena crocea) were identified as Shewanella baltica and Pseudomonas spp. S. baltica was demonstrated as the predominant spoiler in the refrigerated P. crocea due to its high metabolic activities. This work has generated baseline information for a better understanding of the role of various spoilage bacteria in chilled marine fish and for the control of contamination and growth of main spoilage bacteria to extend the shelf life of marine fish.

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Section: Methodsmentioning

confidence: 99%

Spoilage potential characterization ofShewanellaandPseudomonasisolated from spoiled large yellow croaker (Pseudosciaena crocea)

Zhu

et al. 2016

Lett Appl Microbiol

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“…isolated from refrigerated shrimp and large yellow croaker were affected by exogenous AHLs (Zhu et al . , ). However, the QS‐based spoilage mechanism of Shewanella spp.…”

Section: Introductionmentioning

confidence: 99%

Proteomic assessment of the role ofN-acyl homoserine lactone inShewanella putrefaciensspoilage

Zhang

Zhu

Jatt

et al. 2017

Lett Appl Microbiol

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N-acyl homoserine lactone (AHLs)-based quorum sensing signal molecules involving in the behavior regulation in most of the Gram-negative bacteria have widely been reported. This study aims to evaluate the effect of AHLs on Shewanella putrefaciens Z4 at the proteome level. It provides the theoretic basis for elucidating the spoilage mechanism of Shewanella spp., the common spoilage micro-organism in refrigerated marine aquatic food products.

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“…Shewanella can form biofilm on a variety of surfaces, such as ferric oxides, electrodes, stainless steel, and glass (2)(3)(4). This genus is also renowned for an extracellular respiration ability to reduce iron oxide, electrodes, and other extracellular electron acceptors, including heavy metal ions.…”

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confidence: 99%

FlrA Represses Transcription of the Biofilm-Associated bpfA Operon in Shewanella putrefaciens

Cheng

et al. 2017

Appl Environ Microbiol

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Manipulation of biofilm formation in Shewanella is beneficial for application to industrial and environmental biotechnology. BpfA is an adhesin largely responsible for biofilm formation in many Shewanella species. However, the mechanism underlying BpfA production and the resulting biofilm remains vaguely understood. We previously described the finding that BpfA expression is enhanced by DosD, an oxygen-stimulated diguanylate cyclase, under aerobic growth. In the present work, we identify FlrA as a critical transcription regulator of the bpfA operon in Shewanella putrefaciens CN32 by transposon mutagenesis. FlrA acted as a repressor of the operon promoter by binding to two boxes overlapping the Ϫ10 and Ϫ35 sites recognized by 70 . DosD regulation of the expression of the bpfA operon was mediated by FlrA, and cyclic diguanylic acid (c-di-GMP) abolished FlrA binding to the operon promoter. We also demonstrate that FlhG, an accessory protein for flagellum synthesis, antagonized FlrA repression of the expression of the bpfA operon. Collectively, this work demonstrates that FlrA acts as a central mediator in the signaling pathway from c-di-GMP to BpfA-associated biofilm formation in S. putrefaciens CN32.IMPORTANCE Motility and biofilm are mutually exclusive lifestyles, shifts between which are under the strict regulation of bacteria attempting to adapt to the fluctuation of diverse environmental conditions. The FlrA protein in many bacteria is known to control motility as a master regulator of flagellum synthesis. This work elucidates its effect on biofilm formation by controlling the expression of the adhesin BpfA in S. putrefaciens CN32 in response to c-di-GMP. Therefore, FlrA plays a dual role in controlling motility and biofilm formation in S. putrefaciens CN32. The cooccurrence of flrA, bpfA, and the FlrA box in the promoter region of the bpfA operon in diverse Shewanella strains suggests that bpfA is a common mechanism that controls biofilm formation in this bacterial species.KEYWORDS FlrA, Shewanella, biofilm, cyclic di-GMP, transcriptional factor B iofilm formed by bacteria is a multicell architecture for adaptation to diverse niches (1). Shewanella can form biofilm on a variety of surfaces, such as ferric oxides, electrodes, stainless steel, and glass (2-4). This genus is also renowned for an extracellular respiration ability to reduce iron oxide, electrodes, and other extracellular electron acceptors, including heavy metal ions. Such an ability has diverse potential applications in bioengineering and bioremediation (5). Biofilm formation in Shewanella benefits close contact with solid electron acceptors, thereby accelerating iron oxide reduction and improving current output, as well as inducing spatially stratified metabolic responses during contaminant exposure (4, 6, 7). Biofilm formed by Shewanella prevents microbially induced corrosion of steel and cast iron pipes (8, 9). On the other hand, biofilm formation of Shewanella also causes problems in some circumstances. For

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Quorum sensing signals affect spoilage of refrigerated large yellow croaker (Pseudosciaena crocea) by Shewanella baltica

Cited by 88 publications

References 49 publications

Spoilage potential characterization ofShewanellaandPseudomonasisolated from spoiled large yellow croaker (Pseudosciaena crocea)

Spoilage potential characterization ofShewanellaandPseudomonasisolated from spoiled large yellow croaker (Pseudosciaena crocea)

Proteomic assessment of the role ofN-acyl homoserine lactone inShewanella putrefaciensspoilage

FlrA Represses Transcription of the Biofilm-Associated bpfA Operon in Shewanella putrefaciens

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