Quorum Sensing Regulation in Aeromonas hydrophila

Garde, Christian; Bjarnsholt, Thomas; Givskov, Michael; Jakobsen, Tim Holm; Hentzer, Morten; Claussen, Anetta; Sneppen, Kim; Ferkinghoff‐Borg, Jesper; Sams, Thomas

doi:10.1016/j.jmb.2010.01.002

Cited by 37 publications

(40 citation statements)

References 24 publications

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“…For detection of QSI activity, the following reporter strains were used: a QSI selector 1 strain (QSIS1; E. coli) and monitor strains for lasB-gfp, rhlA-gfp (P. aeruginosa), and luxI-gfp (E. coli), described in references 58, 32, 77, and 4, respectively. Production of AHLs was detected by using reporter strains for lasB-gfp (E. coli) (32) and ahyIgfp (E. coli) (30). Animal experiments were performed with the wild-type P. aeruginosa strain PAO1, obtained from Barbara Iglewski (University of Rochester Medical Center, Rochester, NY).…”

Section: Methodsmentioning

confidence: 99%

Ajoene, a Sulfur-Rich Molecule from Garlic, Inhibits Genes Controlled by Quorum Sensing

Jakobsen

Gennip

Phipps

et al. 2012

Antimicrob Agents Chemother

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383

244

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ABSTRACTIn relation to emerging multiresistant bacteria, development of antimicrobials and new treatment strategies of infections should be expected to become a high-priority research area. Quorum sensing (QS), a communication system used by pathogenic bacteria likePseudomonas aeruginosato synchronize the expression of specific genes involved in pathogenicity, is a possible drug target. Previousin vitroandin vivostudies revealed a significant inhibition ofP. aeruginosaQS by crude garlic extract. By bioassay-guided fractionation of garlic extracts, we determined the primary QS inhibitor present in garlic to be ajoene, a sulfur-containing compound with potential as an antipathogenic drug. By comprehensivein vitroandin vivostudies, the effect of synthetic ajoene towardP. aeruginosawas elucidated. DNA microarray studies of ajoene-treatedP. aeruginosacultures revealed a concentration-dependent attenuation of a few but central QS-controlled virulence factors, including rhamnolipid. Furthermore, ajoene treatment ofin vitrobiofilms demonstrated a clear synergistic, antimicrobial effect with tobramycin on biofilm killing and a cease in lytic necrosis of polymorphonuclear leukocytes. Furthermore, in a mouse model of pulmonary infection, a significant clearing of infectingP. aeruginosawas detected in ajoene-treated mice compared to a nontreated control group. This study adds to the list of examples demonstrating the potential of QS-interfering compounds in the treatment of bacterial infections.

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Section: Methodsmentioning

confidence: 99%

Ajoene, a Sulfur-Rich Molecule from Garlic, Inhibits Genes Controlled by Quorum Sensing

Jakobsen

Gennip

Phipps

et al. 2012

Antimicrob Agents Chemother

Self Cite

383

244

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“…This process describes the event whereby an increase in the population density of the bacterial cell is proportional to an increase in the concentration of signal molecule(s) in the extracellular environment (2, 3). Aeromonas hydrophila is QS bacterium and is a known pathogen, as it can cause minor skin infections and gastroenteritis in humans (4, 5). In this study, we report the whole-genome sequence and the QS genes of A.…”

Section: Genome Announcementmentioning

confidence: 99%

Genome Sequence Analysis Reveals Evidence of Quorum-Sensing Genes Present in Aeromonas hydrophila Strain M062, Isolated from Freshwater

et al. 2015

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“…6). Sequenced strains of Aeromonas such as A. hydrophila and Aeromonas salmonicida possess homologs to the canonical LuxI/ R system, the QS regulatory protein in A. hydrophila [21,47]. It is known that some strains of Aeromonas produce short-chain AHLs [21].…”

Section: Effect Of Ahl Addition On Cell Attachment Biofilm Formationmentioning

confidence: 99%

Aeromonas hydrophila produces conductive nanowires

Castro

Vera

Muñoz

et al. 2014

Research in Microbiology

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Quorum Sensing Regulation in Aeromonas hydrophila

Cited by 37 publications

References 24 publications

Ajoene, a Sulfur-Rich Molecule from Garlic, Inhibits Genes Controlled by Quorum Sensing

Ajoene, a Sulfur-Rich Molecule from Garlic, Inhibits Genes Controlled by Quorum Sensing

Genome Sequence Analysis Reveals Evidence of Quorum-Sensing Genes Present in Aeromonas hydrophila Strain M062, Isolated from Freshwater

Aeromonas hydrophila produces conductive nanowires

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