Quorum Sensing Plays a Complex Role in Regulating the Enzyme Hydrolysis Activity of Microbes Associated with Sinking Particles in the Ocean

Krupke, Andreas; Hmelo, Laura R.; Ossolinski, Justin E.; Mincer, Tracy J.; Mooy, Benjamin A. S. Van

doi:10.3389/fmars.2016.00055

Cited by 31 publications

(37 citation statements)

References 48 publications

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“…The metabolic factors that lead to the expression and function of hydrolytic enzymes in marine environments are mostly matters of speculation. Studies suggest that inter‐species interactions and cell numbers (Krupke et al ., ; Cezairliyan and Ausubel, ) can influence the expression of these enzymes. Future work is needed to determine the precise mechanisms, which control the production of hydrolytic enzymes in order to resolve the microbial controls on the marine carbon cycle.…”

Section: Discussionmentioning

confidence: 99%

Illuminating microbial species‐specific effects on organic matter remineralization in marine sediments

Mahmoudi

Enke

Beaupré

et al. 2019

Environmental Microbiology

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Marine microorganisms play a fundamental role in the global carbon cycle by mediating the sequestration of organic matter in ocean waters and sediments. A better understanding of how biological factors, such as microbial community composition, influence the lability and fate of organic matter is needed. Here, we explored the extent to which organic matter remineralization is influenced by species-specific metabolic capabilities. We carried out aerobic time-series incubations of Guaymas Basin sediments to quantify the dynamics of carbon utilization by two different heterotrophic marine isolates (Vibrio splendidus 1A01; Pseudoalteromonas sp. 3D05). Continuous measurement of respiratory CO 2 production and its carbon isotopic compositions ( 13 C and 14 C) shows species-specific differences in the rate, quantity and type of organic matter remineralized. Each species was incubated with hydrothermally-influenced versus unimpacted sediments, resulting in a~2-fold difference in respiratory CO 2 yield across the experiments. Genomic analysis indicated that the observed carbon utilization patterns may be attributed in part to the number of gene copies encoding for extracellular hydrolytic enzymes. Our results demonstrate that the lability and remineralization of organic matter in marine environments is not only a function of chemical composition and/or environmental conditions, but also a function of the microorganisms that are present and active.

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Section: Discussionmentioning

confidence: 99%

Illuminating microbial species‐specific effects on organic matter remineralization in marine sediments

Mahmoudi

Enke

Beaupré

et al. 2019

Environmental Microbiology

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“…Quorum sensing molecules, either retrieved directly from marine snow particles present in surface water or produced by Pantoea ananatis B9, which also colonizes natural snow particles, can enhance the performance of extracellular hydrolytic enzymes such as alkaline phosphatase (Jatt et al, 2015). In fact, hydrolytic pathways involving phosphatases, aminopeptidases and lipases can be influenced within a few hours of contact with acylated homoserine lactones, indicating that microorganisms can dynamically adjust their metabolic pathways as response to quorum sensing (Krupke et al, 2016).…”

Section: Marine Biofilms Protect Bacteria From Harsh Environmental Comentioning

confidence: 99%

Marine Biofilms: A Successful Microbial Strategy With Economic Implications

2018

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Bacteria and other microorganisms have evolved an ingenious form of life, where they cooperate and improve their chances of survival when subjected to environmental stress, called biofilms. In these communities of adhered cells, bacteria are protected by a matrix of extracellular polymeric substances that provide protection against e.g., temperature and pH fluctuations, UV exposure, changes in salinity, depletion of nutrients, antimicrobial compounds, and predation. Their success in marine environments and the number of bacterial cells in the sea, allow them to colonize nearly all man-made surfaces in contact with seawater. The costs to maritime transport, aquaculture, oil and gas industries, desalination plants and other industries are significant which has led to the development of various strategies to prevent biofilm formation and cleaning of infected surfaces. In this review, the benefits for bacterial cells to live in biofilms and the consequences to human activities are discussed.

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“…than isolate quorum sensing pathways, and we discuss details regarding the experimental approach and operational negative controls in prior studiesKrupke et al 2016). …”

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confidence: 99%

The Trichodesmium microbiome can modulate host N₂ fixation

Frischkorn

Rouco

Mooy

et al. 2018

Limnol Oceanogr Letters

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Trichodesmium is a marine, diazotrophic cyanobacterium that plays a central role in the biogeochemical cycling of carbon and nitrogen. Colonies ubiquitously co‐occur with a diverse microbiome of heterotrophic bacteria. We show that manipulation of the microbiome with quorum sensing acyl homoserine lactones (AHLs) significantly modulated rates of N2 fixation by Trichodesmium collected from the western North Atlantic, with positive and negative effects of varied magnitude. Changes in Trichodesmium N2 fixation did not correlate with changes in microbiome composition or geochemistry. With AHL addition, a subset of the significantly differentially expressed genes was related to known quorum sensing responses in model bacteria. However, there was little overlap in specific microbiome transcriptional responses to AHL addition between stations. Overall, these host‐microbiome interactions reflect a complex interplay of biotic and environmental factors that together form an overlooked mechanism modulating Trichodesmium N2 fixation.

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Quorum Sensing Plays a Complex Role in Regulating the Enzyme Hydrolysis Activity of Microbes Associated with Sinking Particles in the Ocean

Cited by 31 publications

References 48 publications

Illuminating microbial species‐specific effects on organic matter remineralization in marine sediments

Illuminating microbial species‐specific effects on organic matter remineralization in marine sediments

Marine Biofilms: A Successful Microbial Strategy With Economic Implications

The Trichodesmium microbiome can modulate host N₂ fixation

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Quorum Sensing Plays a Complex Role in Regulating the Enzyme Hydrolysis Activity of Microbes Associated with Sinking Particles in the Ocean

Cited by 31 publications

References 48 publications

Illuminating microbial species‐specific effects on organic matter remineralization in marine sediments

Illuminating microbial species‐specific effects on organic matter remineralization in marine sediments

Marine Biofilms: A Successful Microbial Strategy With Economic Implications

The Trichodesmium microbiome can modulate host N2 fixation

Contact Info

Product

Resources

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The Trichodesmium microbiome can modulate host N₂ fixation