Quorum sensing pathways in Gram-positive and -negative bacteria: potential of their interruption in abating drug resistance

Haque, Shafiul; Yadav, Dinesh Kumar; Bisht, Shekhar Chandra; Yadav, Neelam; Singh, Vineeta; Dubey, Kashyap Kumar; Jawed, Arshad; Dar, Sajad Ahmad

doi:10.1080/1120009x.2019.1599175

Cited by 44 publications

(39 citation statements)

References 260 publications

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“…Our results showed a significant downregulation of hld following treatment with 1/2 MIC concentration of the CcEO (−3.13-fold). As the QS system is different in Gram-positive and Gram-negative bacteria (different functional molecules and different receptors), the study of the QSI properties in these two groups of bacteria should be done separately ( Haque et al, 2019 ). Interestingly, the QSI activity of this plant against Gram-negative bacteria was reported previously ( Bouyahya et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Cuminum cyminum L. Essential Oil: A Promising Antibacterial and Antivirulence Agent Against Multidrug-Resistant Staphylococcus aureus

et al. 2021

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Cuminum cyminum L. (cumin) is valued for its aromatic and medicinal properties. There are several reports of antibacterial activity of C. cyminum essential oil (CcEO). Accordingly, the present study was conducted to investigate the mechanism(s) of action of the CcEO against multidrug-resistant (MDR) Staphylococcus aureus. Therefore, 10 S. aureus MDR isolates, obtained from different sources, were selected based on the antibiotic susceptibility patterns and the Clinical and Laboratory Standards Institute definition and subjected to the examinations. Our results exhibited promising bacteriostatic and bactericidal properties of the CcEO. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration values ranged from 5 to 10 and 10 to 20 μL ⋅ mL–1, respectively. Scanning electron microscope was used to assess the bacterial cell structure and morphology after the induction with 1/2 MIC concentration of the CcEO. The observed morphological changes appeared to be deformation of the cell membrane and destruction of the cells. In the case of quorum sensing inhibitory potential, treatment of S. aureus isolates with the sub-MIC concentrations (1/2 MIC) of the CcEO significantly reduced the hld expression (3.13-fold downregulation), which considerably controls S. aureus quorum-sensing accessory regulator system. Another virulence factor influenced by the CcEO was the polysaccharide intercellular adhesion production system, as an important component of cell–cell adhesion and biofilm formation. Consequently, the expression level of the intercellular adhesion (ica) locus in the S. aureus cells was examined following treatment with CcEO. The results showed significant decrease (−3.3-fold) in ica expression, indicating that the CcEO could potentially interfere with the process of biofilm formation. Using the ethidium bromide efflux inhibition assay, the S. aureus NorA efflux pump was phenotypically but not genotypically (in quantitative polymerase chain reaction assay) affected by the CcEO treatment. Using gas chromatography–mass spectrometry analysis, cuminic aldehyde (38.26%), α,β-dihydroxyethylbenzene (29.16%), 2-caren-10-al (11.20%), and γ-terpinene (6.49%) were the most detected compounds. The antibacterial and antivirulence action of the CcEO at sub-MIC concentrations means that no microbial resistance will be promoted and developed after the treatment with this agent. These findings revealed that the CcEO is a promising antibacterial agent to control infections caused by the MDR S. aureus strains.

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Section: Discussionmentioning

confidence: 99%

Cuminum cyminum L. Essential Oil: A Promising Antibacterial and Antivirulence Agent Against Multidrug-Resistant Staphylococcus aureus

et al. 2021

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“…As cell density increases, AIs accumulate in the surroundings ( Figure 5 ). Once this increase reaches a threshold concentration, often referred to as quorum level, response regulator proteins part of the QS systems become activated by binding to AI molecules, leading to the modulation of gene transcription (for review, see Haque et al., 2019 ). As a consequence of this intercellular communication mechanism, bacteria become synchronized to express particular QS phenotypic profiles.…”

Section: Fundamental Concepts On Biofilm Developmentmentioning

confidence: 99%

“…As a consequence of this intercellular communication mechanism, bacteria become synchronized to express particular QS phenotypic profiles. There are several QS systems that may be shared between different bacterial species or may be specific for certain species in particular (for review, see Haque et al., 2019 and Waters and Bassler, 2005 ). It has been widely reported that QS contributes to biofilm development, from the formation of microcolonies to biofilm maturation and dispersal ( Boles and Horswill, 2008 ; Davies et al., 1998 ; de Kievit et al., 2001 ), mainly by influencing its architecture and promoting a series of beneficial phenotypic traits.…”

Section: Fundamental Concepts On Biofilm Developmentmentioning

confidence: 99%

Mimicking biofilm formation and development: Recent progress in in vitro and in vivo biofilm models

et al. 2021

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Biofilm formation in living organisms is associated to tissue and implant infections, and it has also been linked to the contribution of antibiotic resistance. Thus, understanding biofilm development and being able to mimic such processes is vital for the successful development of antibiofilm treatments and therapies. Several decades of research have contributed to building the foundation for developing in vitro and in vivo biofilm models. However, no such thing as an ''all fit'' in vitro or in vivo biofilm models is currently available. In this review, in addition to presenting an updated overview of biofilm formation, we critically revise recent approaches for the improvement of in vitro and in vivo biofilm models.

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“…Given the importance of quorum sensing in the bacterial virulence and biolm formation, its inhibition has emerged as an important strategy to tackle the menace of MDR and pan-drug-resistant (PDR) bacterial pathogens. 19,20 The concept is very simple. By interfering with their virulence factors and not killing them via inhibition of quorum sensing, we are in a way slowing down the emergence of MDR and PDR strain.…”

Section: Introductionmentioning

confidence: 99%

Potassium 2-methoxy-4-vinylphenolate: a novel hit exhibiting quorum-sensing inhibition inPseudomonas aeruginosa viaLasIR/RhlIR circuitry

et al. 2019

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Quorum sensing pathways in Gram-positive and -negative bacteria: potential of their interruption in abating drug resistance

Cited by 44 publications

References 260 publications

Cuminum cyminum L. Essential Oil: A Promising Antibacterial and Antivirulence Agent Against Multidrug-Resistant Staphylococcus aureus

Cuminum cyminum L. Essential Oil: A Promising Antibacterial and Antivirulence Agent Against Multidrug-Resistant Staphylococcus aureus

Mimicking biofilm formation and development: Recent progress in in vitro and in vivo biofilm models

Potassium 2-methoxy-4-vinylphenolate: a novel hit exhibiting quorum-sensing inhibition inPseudomonas aeruginosa viaLasIR/RhlIR circuitry

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