Quorum Sensing Inhibition in Chromobacterium violaceum by Amikacin Combination with Activated Charcoal or Small Plant-Derived Molecules (Pyrogallol and Coumarin)

Inchagova, K. S.; Дускаев, Г. К.; Deryabin, D. G.

doi:10.1134/s0026261719010132

Cited by 14 publications

(10 citation statements)

References 27 publications

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“…2,4-Diacetylphloroglucinol also demonstrated a subinhibitory effect on quorum-sensing (QS) mediated behavior of bacterial cells. The possibility that 2,4-DAPG is capable of inhibiting the processes associated with QS follows indirectly from the structural similarities with known QS-inhibitors such as eugenol, carvacrol, pyrogallol, and resveratrol ( Figure 9 a–d) [ 31 , 32 , 33 ]. Moreover, it was shown that phloroglucinol is capable of functioning as a chemical messenger regulating gene expression in Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Bacterial-Derived Plant Protection Metabolite 2,4-Diacetylphloroglucinol: Effects on Bacterial Cells at Inhibitory and Subinhibitory Concentrations

et al. 2020

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2,4-Diacetylphloroglucinol (2,4-DAPG) is a well-known bacterial secondary metabolite, however, its mechanism of inhibitory and subinhibitory action on bacterial cells is still poorly understood. The mechanism of 2,4-DAPG action on model bacterial strains was investigated using fluorescent spectroscopy and the action of the antibiotic was found to involve a rapid increase in membrane permeability that was accompanied by a reduction in its viability in nutrient-poor medium. At the same time, antibacterial action in nutrient-rich medium developed for several hours. Atomic force microscopy demonstrated time-dependent disturbances in the outer membrane of Escherichia coli when exposed to 2,4-DAPG, while Staphylococcusaureus cells have been visualized with signs of intracellular leakage. In addition, 2,4-DAPG inhibited the metabolic activity of S. aureus and E. coli bacterial cells in mature biofilms. Observed differences in the antibiofilm activity were dependent upon antibiotic concentration. The intracellular targets of the action of 2,4-DAPG were assessed using bacterial biosensors with inducible bioluminescence corresponding to DNA and protein damage. It was unable to register any positive response from either sensor. As a result, the bactericidal action of 2,4-DAPG is believed to be associated with the destruction of the bacterial barrier structures. The subinhibitory effect of 2,4-diacetylphloroglucinol was tested on quorum-sensing mediated processes in Pectobacterium carotovorum. Subinhibitory concentrations of 2,4-DAPG were found to lower the biosynthesis of acyl-homoserine lactones in P. carotovorum in a dose-dependent manner. Further investigation elucidated that 2,4-DAPG inhibits the metabolic activity of bacteria without affecting their viability.

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Section: Discussionmentioning

confidence: 99%

Bacterial-Derived Plant Protection Metabolite 2,4-Diacetylphloroglucinol: Effects on Bacterial Cells at Inhibitory and Subinhibitory Concentrations

et al. 2020

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“…The pH-dependent hydrolysis is reversible when pH was adjusted to < 2, yet prolonged incubation times were required to recyclize the HSL rings (Yates et al 2002). With both sorption and hydrolysis, biochar and charcoal reduce the extracellular concentration of the AHLs, consequently interrupting the modification of cell density by AHLs (Inchagcova et al 2019;Zhu et al 2017b). The effects and mechanisms of biochar application on microbial communication in terms of quorum quenching are far from well-known.…”

Section: Biochar-microbe Interactionsmentioning

confidence: 99%

Application of biochar-based materials in environmental remediation: from multi-level structures to specific devices

Wang

et al. 2020

Biochar

136

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The development of biochar has triggered a hot-spot in various research fields including agriculture, energy, environment, and materials. Biochar-based materials provide a novel approach against environmental challenging issues. Considering the rapid development of biochar materials, this review serves as a valuable platform to summarize the recent progress on the theoretical investigation and engineering applications of biochar materials in environmental remediation. For a better understanding of the structure-application relationships, the structural properties of biochar from macroscopic and microscopic aspects are summarized. The multilevel structures including elements, phases, surface chemistry, and molecular are highlighted to elucidate the multi-functional properties of biochars. Sorption, catalysis, redox reaction, and biological activity of biochar are briefly illustrated, which influence the transport, transformation, and removal of organic and inorganic pollutants in the environments. According to the multi-level structures and structure-application relationships of biochar, specific biocharbased materials and devices have been designed for practical environmental application. The important progress on the functionalization and device of biochar-based materials, including magnetic biochars, 2D and 3D biochar-based macrostructures, immobilized microorganism on biochar, and biochar-amended biofilters are highlighted. The environmental friendliness and sustainability of biochar-based materials, considering the whole cycle from synthesis to application, are evaluated.

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“…The aim of this study was to investigate the separate and joint action of the probiotic Bacillus cereus (BC), with anti-quorum activity [ 25 ], and CO derived earlier from an oak bark extract [ 26 ] on broiler productivity, biochemical indicators of blood, and muscular and liver tissues.…”

Section: Introductionmentioning

confidence: 99%

Effects of Bacillus cereus and coumarin on growth performance, blood biochemical parameters, and meat quality in broilers

2020

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Background and Aim: Progressive antibiotic resistance has become the primary threat to public health. The search for alternative substances with similar effects is now a global challenge for poultry farming. The aim of this study was to investigate the action of the probiotic Bacillus cereus (BC) and coumarin (CO) on broiler productivity, biochemical indicators of blood, and muscular and liver tissues. Materials and Methods: The trial of this study included Arbor Acres cross broiler chickens that were grown up to the age of 42 days. The experiment was conducted on 200 broiler chickens divided into four experimental groups of 50 individuals each: The control group received ration without additives (main ration [MR]), the first experimental group received MR+BC, the second received MR+CO, and the third received –MR+BC+CO. A biochemical and hematological analyzer was used to estimate elemental concentrations using inductively coupled plasma mass spectrometry and inductively coupled plasma atomic emission spectrometry. Results: Inclusion of CO and CO+BC in the diet improved growth rates and reduced feed consumption (FC) per kg of live weight gain. Decreased white blood cell count, increased creatinine and triglycerides (CO), changes in aminotransferase and transpeptidase activity, and increases in chemical elements in the liver and pectoral muscles (BC+CO) were observed. The inclusion of BC+CO in the diet contributed to increases in a greater number of chemical elements in the liver (calcium [Ca], K, magnesium, Mn, Si, and Zn) and the pectoral muscles (Ca, Na, Co, Cu, Fe, Mn, Ni, and Zn). Conclusion: The inclusion of CO and CO+BC in the diet improves growth rates and reduces FC in broilers against a background of the absence of mortality during the experiment.

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Quorum Sensing Inhibition in Chromobacterium violaceum by Amikacin Combination with Activated Charcoal or Small Plant-Derived Molecules (Pyrogallol and Coumarin)

Cited by 14 publications

References 27 publications

Bacterial-Derived Plant Protection Metabolite 2,4-Diacetylphloroglucinol: Effects on Bacterial Cells at Inhibitory and Subinhibitory Concentrations

Bacterial-Derived Plant Protection Metabolite 2,4-Diacetylphloroglucinol: Effects on Bacterial Cells at Inhibitory and Subinhibitory Concentrations

Application of biochar-based materials in environmental remediation: from multi-level structures to specific devices

Effects of Bacillus cereus and coumarin on growth performance, blood biochemical parameters, and meat quality in broilers

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