Quorum sensing inhibition activity of garlic extract andp-coumaric acid

Bodini, Sergio F.; Manfredini, Stefano; Epp, M.; Valentini, Sabina; Santori, Fabio R.

doi:10.1111/j.1472-765x.2009.02704.x

Cited by 116 publications

(71 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to the crucial role of QS systems in regulating virulence and biofilm formation, inhibition of the QS systems provides an alternative approach for treating P. aeruginosa infections. Extensive studies have focused on searching for drugs capable of blocking QS and attenuating pathogenicity.Dimethyl sulfoxide (DMSO) is an important polar aprotic solvent that is frequently used as a vehicle in both in vivo and in vitro experiments, including in quorum sensing inhibitor (QSI) screening studies (17)(18)(19)(20). It was unexpectedly observed during our experiments that DMSO could significantly inhibit the production of the pigmented compound pyocyanin, an important pathogenic factor in P. aeruginosa that is tightly regulated by the QS systems.…”

mentioning

confidence: 85%

Potential Use of Dimethyl Sulfoxide in Treatment of Infections Caused by Pseudomonas aeruginosa

Qiao

Bai

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

c Dimethyl sulfoxide (DMSO) is commonly used as a solvent to dissolve water-insoluble drugs or other test samples in both in vivo and in vitro experiments. It was observed during our experiment that DMSO at noninhibitory concentrations could significantly inhibit pyocyanin production in the human pathogen Pseudomonas aeruginosa. Pyocyanin is an important pathogenic factor whose production is controlled by a cell density-dependent quorum-sensing (QS) system. Investigation of the effect of DMSO on QS showed that DMSO has significant QS antagonistic activities and concentrations of DMSO in the micromolar range attenuated a battery of QS-controlled virulence factors, including rhamnolipid, elastase, and LasA protease production and biofilm formation. Further study indicated that DMSO inhibition of biofilm formation and pyocyanin production was attained by reducing the level of production of an autoinducer molecule of the rhl QS system, N-butanoyl-L-homoserine lactone (C 4 -HSL). In a mouse model of a burn wound infection with P. aeruginosa, treatment with DMSO significantly decreased mouse mortality compared with that for mice in the control group. The capacity of DMSO to attenuate the pathogenicity of P. aeruginosa points to the potential use of DMSO as an antipathogenic agent for the treatment of P. aeruginosa infection. As a commonly used solvent, however, DMSO's impact on bacterial virulence calls for cautionary attention in its usage in biological, medicinal, and clinical studies. Pseudomonas aeruginosa is a prevalent opportunistic pathogen capable of causing various infections in humans, including pneumonia and urinary tract infections, bloodstream infections, and infections in burn patients (1). The chronic infection caused by P. aeruginosa and the associated pulmonary inflammation are ultimately responsible for the majority of cases of mortality in patients with cystic fibrosis (2). The ability of P. aeruginosa to cause diverse infections is attributed to its myriad virulence factors and biofilm-forming capability, which are controlled by the intercellular quorum-sensing (QS) communication system (3-5).P. aeruginosa has two acyl-homoserine lactone (AHL)-mediated QS systems, known as the las and rhl QS systems. The las and rhl systems consist of the transcriptional activators LasR and RhlR, respectively, and the signal synthases LasI and RhlI, respectively. The major signals in the las and rhl systems are N-(3-oxododecanoyl)-HSL (3-oxo-C 12 -HSL) and N-butanoyl-L-homoserine lactone (C 4 -HSL), respectively (5). P. aeruginosa employs these QS systems to control a wide range of extracellular virulence factors, including pyocyanin, elastase, and rhamnolipid (7-12). The AHL-mediated QS systems also play a crucial role in biofilm formation by P. aeruginosa, a common cause of resistance to antibiotics and the difficulties with the treatment of infections (13). The las system influences the activation of pel and, accordingly, biofilm matrix formation (14), and the rhl system contributes to the maintenance of the bio...

show abstract

mentioning

confidence: 85%

Potential Use of Dimethyl Sulfoxide in Treatment of Infections Caused by Pseudomonas aeruginosa

Qiao

Bai

et al. 2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Extracts of fruits (e.g., blackberries, cranberries, vanilla, and citrus) and herbs (e.g., rosemary and turmeric) and extracts and oils of other plant materials (garlic, clove, and cinnamon) and medicinal plants (e.g., betel nut and notoginsing) have all displayed QSI activity to some degree, typically found via inhibition of violacein production in the C. violaceum bioassay (137,138,(264)(265)(266)(267)(268)(269)(270)(271)(272)(273)(274)(275). In very few cases, however, have individual compounds been isolated or identified from these kinds of complex sources.…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

676

556

View full text Add to dashboard Cite

SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

show abstract

“…Some studies suggest P-coumaric acid has a significant role in the anti-bacterial activity and interference quorum sensing system [25,26,27]. Quercetin was found to inhibit biofilm formation by V. harveyi BB120 and E. coli O157:H7 [28].…”

Section: Discussionmentioning

confidence: 99%

“…The cultures on coverslips were rinsed with phosphate buffer at each interval. The cultures on coverslips were dehydrated in a gradient alcohol concentration (25,50,70, 80 90 and 100 %) for 15 min at each concentration [17], and the cultures dehydrated twice in 100 % ethanol. Lastly, they were covered with gold before examination.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Chemical composition and anti-biofilm activity of burdock (Arctium lappa L Asteraceae) leaf fractions against Staphylococcus aureus

Tang¹,

Lou²,

Rahman³

et al. 2014

Trop. J. Pharm Res

View full text Add to dashboard Cite

Purpose: To determine the chemical composition and anti-biofilm activity of burdock leaf fractions against Staphylococcus aureus Methods: The anti-biofilm activity of burdock leaf fractions obtained by column chromatography against S. aureus was determined by minimum inhibitory concentration (MIC). Scanning electron microscopy was employed to further investigate the inhibitory activity. Analysis of the chemical composition of the fractions was performed by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS

show abstract

Quorum sensing inhibition activity of garlic extract andp-coumaric acid

Cited by 116 publications

References 18 publications

Potential Use of Dimethyl Sulfoxide in Treatment of Infections Caused by Pseudomonas aeruginosa

Potential Use of Dimethyl Sulfoxide in Treatment of Infections Caused by Pseudomonas aeruginosa

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Chemical composition and anti-biofilm activity of burdock (<i>Arctium lappa</i> L Asteraceae) leaf fractions against <i>Staphylococcus aureus</i>

Contact Info

Product

Resources

About