Quorum Sensing Inhibiting Activity of Streptomyces coelicoflavus Isolated from Soil

Hassan, Ramadan; Shaaban, Mona I.; Bar, Fatma M. Abdel; El-Mahdy, Areej M.; Shokralla, Shadi

doi:10.3389/fmicb.2016.00659

Cited by 72 publications

(37 citation statements)

References 61 publications

(62 reference statements)

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“…Finally, our study confirmed the existence of predatory activity in Streptomyces species. S. griseoflavus EMM111, S. coelicoflavus EMM112, and S. mutabilis EMM183 were already known as a terrestrial strain of Streptomyces [61][62][63], whereas S. champavatii EMM194 was previously isolated from a marine environment [64]. Some marine Actinobacteria can indeed have a terrestrial origin [65], being transported from a terrestrial to a marine environment by rivers, rainfall, wind, and spores leaching [65,66].…”

Section: Discussionmentioning

confidence: 99%

Marine Actinobacteria: Screening for Predation Leads to the Discovery of Potential New Drugs against Multidrug-Resistant Bacteria

et al. 2020

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Predatory bacteria constitute a heterogeneous group of prokaryotes able to lyse and feed on the cellular constituents of other bacteria in conditions of nutrient scarcity. In this study, we describe the isolation of Actinobacteria predator of other bacteria from the marine water of the Moroccan Atlantic coast. Only 4 Actinobacteria isolates showing strong predation capability against native or multidrug-resistant Gram-positive or Gram-negative bacteria were identified among 142 isolated potential predatory bacteria. These actinobacterial predators were shown to belong to the Streptomyces genus and to inhibit the growth of various native or multidrug-resistant micro-organisms, including Micrococcus luteus, Staphylococcus aureus (native and methicillin-resistant), and Escherichia coli (native and ampicillin-resistant). Even if no clear correlation could be established between the antibacterial activities of the selected predator Actinobacteria and their predatory activity, we cannot exclude that some specific bio-active secondary metabolites were produced in this context and contributed to the killing and lysis of the bacteria. Indeed, the co-cultivation of Actinobacteria with other bacteria is known to lead to the production of compounds that are not produced in monoculture. Furthermore, the production of specific antibiotics is linked to the composition of the growth media that, in our co-culture conditions, exclusively consisted of the components of the prey living cells. Interestingly, our strategy led to the isolation of bacteria with interesting inhibitory activity against methicillin-resistant S. aureus (MRSA) as well as against Gram-negative bacteria.

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Section: Discussionmentioning

confidence: 99%

Marine Actinobacteria: Screening for Predation Leads to the Discovery of Potential New Drugs against Multidrug-Resistant Bacteria

et al. 2020

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“…Since quorum sensing plays a vital role in biofilm formation, it can be used as a potential drug target. In fact, studies have been carried out screening Streptomyces extracts that can inhibit the quorum sensing pathway in some gram positive and gram-negative bacteria (Hassan et al, 2016 ; Miao et al, 2017 ).…”

Section: Anti-biofilm and Anti-virulence Activitiesmentioning

confidence: 99%

Streptomyces as a Prominent Resource of Future Anti-MRSA Drugs

et al. 2018

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Methicillin-resistant Staphylococcus aureus (MRSA) pose a significant health threat as they tend to cause severe infections in vulnerable populations and are difficult to treat due to a limited range of effective antibiotics and also their ability to form biofilm. These organisms were once limited to hospital acquired infections but are now widely present in the community and even in animals. Furthermore, these organisms are constantly evolving to develop resistance to more antibiotics. This results in a need for new clinically useful antibiotics and one potential source are the Streptomyces which have already been the source of several anti-MRSA drugs including vancomycin. There remain large numbers of Streptomyces potentially undiscovered in underexplored regions such as mangrove, deserts, marine, and freshwater environments as well as endophytes. Organisms from these regions also face significant challenges to survival which often result in the production of novel bioactive compounds, several of which have already shown promise in drug development. We review the various mechanisms of antibiotic resistance in MRSA and all the known compounds isolated from Streptomyces with anti-MRSA activity with a focus on those from underexplored regions. The isolation of the full array of compounds Streptomyces are potentially capable of producing in the laboratory has proven a challenge, we also review techniques that have been used to overcome this obstacle including genetic cluster analysis. Additionally, we review the in vivo work done thus far with promising compounds of Streptomyces origin as well as the animal models that could be used for this work.

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“…Medicinal plants 6 and edible plants 7 exhibited QSI effects. Also, Streptomyces coelicoflavus isolated from soil microbiota produced 1 H-pyrrole-2-carboxylic acid with QSI effect 8 . Synthetic molecules and peptides exhibited QSI activity 9 .…”

Section: Introductionmentioning

confidence: 99%

Quorum sensing inhibitory activity of sub-inhibitory concentrations of β-lactams

El-Mowafy

Galil

Habib³

et al. 2017

Afr H. Sci.

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Introduction: The virulence factors of Pseudomonas aeruginosa are under the control of quorum sensing (QS) signals. Hence, interference with QS prevents its pathogenesis. Objective: The aim of the present research is to assess the influence of some β-lactam antibiotics on cell communication and the release of different virulence factors. Methods: The minimal inhibitory concentrations of ceftazidime, cefepime and imipenem were evaluated by microbroth dilution method. The effect of sub-inhibitory concentration of the tested antibiotics on QS signals was investigated using reporter strain assay. In addition, different virulence factors (elastase, protease, pyocyanin and hemolysin) were estimated in the presence of their sub-inhibitory concentrations. Results: Low concentrations of ceftazidime, cefepime and imipenem caused significant elimination of the QS signals 3OH-C12-HSL and C4-HSL up to 1/20 MIC. Furthermore, low concentrations of the tested antimicrobials suppressed virulence factors elastase and hemolysin. Moreover, 1/20 of their MICs reduced elastase, protease, pyocyanin and hemolysin. Conclusion: Utilization of β-lactam antibiotics at low concentrations could be an effective approach for prevention and treatment of P. aeruginosa infection.

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Quorum Sensing Inhibiting Activity of Streptomyces coelicoflavus Isolated from Soil

Cited by 72 publications

References 61 publications

Marine Actinobacteria: Screening for Predation Leads to the Discovery of Potential New Drugs against Multidrug-Resistant Bacteria

Marine Actinobacteria: Screening for Predation Leads to the Discovery of Potential New Drugs against Multidrug-Resistant Bacteria

Streptomyces as a Prominent Resource of Future Anti-MRSA Drugs

Quorum sensing inhibitory activity of sub-inhibitory concentrations of β-lactams

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