Quorum Sensing-Independent Cellulase-Sensitive Pellicle Formation Is Critical for Colonization of Burkholderia glumae in Rice Plants

Kwak, Gi-Young; Choi, Okhee; Goo, Eunhye; Kang, Yong-Cheol; Kim, Jinwoo; Hwang, Ingyu

doi:10.3389/fmicb.2019.03090

Cited by 13 publications

(12 citation statements)

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“…Pellicle formation of B. glumae BGR1 was the first behavioral change observed in static culture [21]. The present study demonstrated that cells of B. glumae change genetic elements to The levels of c-di-GMP were elevated in the bspP null mutant BGP38 compared to those of wild-type BGR1 and the complementation strain BGP38C.…”

Section: Discussionsupporting

confidence: 48%

“…Pellicle formation of B . glumae BGR1 was the first behavioral change observed in static culture [ 21 ]. The present study demonstrated that cells of B .…”

Section: Discussionmentioning

confidence: 99%

“…Virulence and colonization assays of B. glumae strains in Oryza sativa cv. Milyang 23 were performed as described previously [21].…”

Section: Plant Inoculationmentioning

confidence: 99%

“…We believe our results have at least partially answered these questions. Pellicle formation is a collective behavior of B. glumae that provides a survival advantage in static culture [21]. However, the genetic and physiological natures of cells in static culture were not homogeneous.…”

Section: Plos Onementioning

confidence: 99%

“…Such detoxification is essential for the survival of B. glumae when grown in amino acid-rich media [19]. We previously reported that B. glumae produces cellulase-sensitive pellicles dependent on bis-(3 0 -5 0 )-cyclic dimeric guanosine monophosphate (c-di-GMP) but independent of QS in static culture [21], which led us to investigate how cells of B. glumae behave physiologically and genetically in a pellicle-forming environment.…”

Section: Introductionmentioning

confidence: 99%

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Adverse effects of adaptive mutation to survive static culture conditions on successful fitness of the rice pathogen Burkholderia glumae in a host

et al. 2020

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Bacteria often possess relatively flexible genome structures and adaptive genetic variants that allow survival in unfavorable growth conditions. Bacterial survival tactics in disadvantageous microenvironments include mutations that are beneficial against threats in their niche. Here, we report that the aerobic rice bacterial pathogen Burkholderia glumae BGR1 changes a specific gene for improved survival in static culture conditions. Static culture triggered formation of colony variants with deletions or point mutations in the gene bspP (BGLU_RS28885), which putatively encodes a protein that contains PDC2, PAS-9, SpoIIE, and HATPase domains. The null mutant of bspP survived longer in static culture conditions and produced a higher level of bis-(3 0-5 0)-cyclic dimeric guanosine monophosphate than the wild type. Expression of the bacterial cellulose synthase regulator (bcsB) gene was upregulated in the mutant, consistent with the observation that the mutant formed pellicles faster than the wild type. Mature pellicle formation was observed in the bspP mutant before pellicle formation in wild-type BGR1. However, the population density of the bspP null mutant decreased substantially when grown in Luria-Bertani medium with vigorous agitation due to failure of oxalate-mediated detoxification of the alkaline environment. The bspP null mutant was less virulent and exhibited less effective colonization of rice plants than the wild type. All phenotypes caused by mutations in bspP were recovered to those of the wild type by genetic complementation. Thus, although wild-type B. glumae BGR1 prolonged viability by spontaneous mutation under static culture conditions, such genetic changes negatively affected colonization in rice plants. These results suggest that adaptive gene sacrifice of B. glumae to survive unfavorable growth conditions is not always desirable as it can adversely affect adaptability in the host.

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Section: Discussionsupporting

confidence: 48%

“…Pellicle formation of B . glumae BGR1 was the first behavioral change observed in static culture [ 21 ]. The present study demonstrated that cells of B .…”

Section: Discussionmentioning

confidence: 99%

“…Virulence and colonization assays of B. glumae strains in Oryza sativa cv. Milyang 23 were performed as described previously [21].…”

Section: Plant Inoculationmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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