Quorum sensing autoinducers AHLs protect Shewanella baltica against phage infection

Xuan, Guanhua; Tan, Lin; Yang, Yuqing; Kong, Jiuna; Lin, Hong; Wang, Jingxue

doi:10.1016/j.ijfoodmicro.2023.110304

Cited by 2 publications

(2 citation statements)

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“…Since P. aeruginosa has a complex QS network, which may play an important role in its adaptation ability, regulating biofilm and pyocyanin production, motility, and other virulence factors ( 45 ), we selected these two strains TL3780-R1 and TL3780-R2 for subsequent QS gene expression detection. Xuan et al also reported that AHL produced by the quorum-sensing system can regulate its resistance to phages, and AHL can reduce phage adsorption by down-regulating LPS expression, thus resisting phage infection ( 46 ). The results of RT-qPCR showed that compared with TL3780, the QS gene expression of TL3780-R1 and TL3780-R2 was up-regulated.…”

Section: Discussionmentioning

confidence: 99%

Resistance, mechanism, and fitness cost of specific bacteriophages for Pseudomonas aeruginosa

Feng,

Chen,

Qian

et al. 2024

mSphere

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The bacteriophage is an effective adjunct to existing antibiotic therapy; however, in the course of bacteriophage therapy, host bacteria will develop resistance to bacteriophages, thus affecting the efficacy. Therefore, it is important to describe how bacteria evade bacteriophage attack and the consequences of the biological changes that accompany the development of bacteriophage resistance before the bacteriophage is applied. The specific bacteriophage vB3530 of Pseudomonas aeruginosa ( P. aeruginosa ) has stable biological characteristics, short incubation period, strong in vitro cleavage ability, and absence of virulence or resistance genes. Ten bacteriophage-resistant strains (TL3780-R) were induced using the secondary infection approach, and the plaque assay showed that vB3530 was less sensitive to TL3780-R. Identification of bacteriophage adsorption receptors showed that the bacterial surface polysaccharide was probably the adsorption receptor of vB3530. In contrast to the TL3780 parental strain, TL3780-R is characterized by the absence of long lipopolysaccharide chains, which may be caused by base insertion of wzy or deletion of galU . It is also intriguing to observe that, in comparison to the parent strain, the bacteriophage-resistant strains TL3780-R mostly exhibited a large cost of fitness (growth rate, biofilm formation, motility, and ability to produce enhanced pyocyanin). In addition, TL3780-R9 showed increased susceptibility to aminoglycosides and chlorhexidine, which may be connected to the loss and down-regulation of mexX expression. Consequently, these findings fully depicted the resistance mechanism of P. aeruginosa to vB3530 and the fitness cost of bacteriophage resistance, laying a foundation for further application of bacteriophage therapy. IMPORTANCE The bacteriophage is an effective adjunct to existing antibiotic therapy; However, bacteria also develop defensive mechanisms against bacteriophage attack. Thus, there is an urgent need to deeply understand the resistance mechanism of bacteria to bacteriophages and the fitness cost of bacteriophage resistance so as to lay the foundation for subsequent application of the phage. In this study, a specific bacteriophage vB3530 of P. aeruginosa had stable biological characteristics, short incubation period, strong in vitro cleavage ability, and absence of virulence or resistance genes. In addition, we found that P. aeruginosa may lead to phage resistance due to the deletion of galU and the base insertion of wzy , involved in the synthesis of lipopolysaccharides. Simultaneously, we showed the association with the biological state of the bacteria after bacteria acquire bacteriophage resistance, which is extremely relevant to guide the future application of therapeutic bacteriophages.

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Section: Discussionmentioning

confidence: 99%

Resistance, mechanism, and fitness cost of specific bacteriophages for Pseudomonas aeruginosa

Feng,

Chen,

Qian

et al. 2024

mSphere

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“…In E. coli , the QS system reduced phage receptors [ 16 , 31 ]. Additionally, the study by Xuan et a l. revealed that N-acyl homoserine lactones (AHLs) protected Shewanella baltica from phage infection by decreasing LPS-mediated phage adsorption [ 32 ]. The above studies all hypothesized that QS negatively regulates the expression of receptors required for phage infection.…”

Section: Discussionmentioning

confidence: 99%

Quorum sensing gene lasR promotes phage vB_Pae_PLY infection in Pseudomonas aeruginosa

Liu,

Yao,

Mao

et al. 2024

BMC Microbiol

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Background Quorum sensing (QS) is a cell density-based intercellular communication system that controls virulence gene expression and biofilm formation. In Pseudomonas aeruginosa (P. aeruginosa), the LasR system sits at the top of the QS hierarchy and coordinates the expression of a series of important traits. However, the role of lasR in phage infection remains unclear. This study aims to investigate the role of lasR QS in phage infection. Methods The P. aeruginosa phage was isolated from sewage, and its biological characteristics and whole genome were analyzed. The adsorption receptor was identified via a phage adsorption assay. Following lasR gene knockout, the adsorption rate and bactericidal activity of phage were analyzed. Finally, real-time quantitative polymerase chain reaction (RT-qPCR) was conducted to explore how lasR promoting phage infection. Results The lytic phage vB_Pae_PLY was isolated and lipopolysaccharide (LPS) was identified as its adsorption receptor. The adsorption rate and bactericidal activity of vB_Pae_PLY were reduced after lasR knockout. RT-qPCR results showed that the expression of galU, a key gene involved in LPS synthesis, was down-regulated, and several genes related to type IV pili (T4P) were also down-regulated in the lasR mutant PaΔlasR. Conclusions The study showed that QS lasR may promote phage vB_Pae_PLY infection by involving in the synthesis of LPS and T4P. This study provides an example of QS in promoting phage infection and deepens the understanding of phage-bacteria interactions.

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Quorum sensing autoinducers AHLs protect Shewanella baltica against phage infection

Cited by 2 publications

References 46 publications

Resistance, mechanism, and fitness cost of specific bacteriophages for Pseudomonas aeruginosa

Resistance, mechanism, and fitness cost of specific bacteriophages for Pseudomonas aeruginosa

Quorum sensing gene lasR promotes phage vB_Pae_PLY infection in Pseudomonas aeruginosa

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