Quorum Sensing and Bacterial Social Interactions in Biofilms

Li, Yung-Hua; Tian, Xiaolin

doi:10.3390/s120302519

Cited by 559 publications

(394 citation statements)

References 80 publications

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“…PJI is a condition with high morbidity and costly economic side effects for endoprosthetic departments [1,2]. The complex pathophysiology of PJI is closely related to the unique microbiological properties of biofilms, generated by microorganisms on artificial materials [3]. Biofilmforming bacteria often remain in a dormant state complicating conventional diagnostic and therapeutic approaches [4].…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of prosthetic joint infections using UMD-Universal Kit and the automated multiplex-PCR Unyvero i60 ITI® cartridge system: a pilot study

et al. 2015

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cultures of periprosthetic tissue samples were compared with the results of broad-range 16S rRNA gene real-time PCR (UMD-Universal Pathogen DNA Extraction and PCR Analysis, Molzym GmbH, Germany) and the multiplex-PCR Unyvero ITI ® cartridge system (U-ITI; Curetis AG, Germany). Conventional culture and broad-range 16S rRNA gene real-time PCR were performed on all samples. U-ITI was used in a subgroup of 28 cases including all culture-positive cases. The agreement of the results from the methods was assessed. Results Of 54 cases, seven were culture-positive. Broadrange 16S rRNA gene real-time PCR gave 6, U-ITI 3 concordant positive results. Of the 47 culture-negative samples, 46 were also negative by broad-range 16S rRNA gene realtime PCR resulting in a 96 % (52/54) agreement between 16S rRNA gene PCR and culture. Of the 21 culture-negative samples analysed with U-ITI, 20 gave negative results, including the single 16S rRNA gene PCR-positive/culturenegative specimen. The rate of agreement between U-ITI and culture results was 82 % (23/28). Conclusion This pilot study gave no indication of superiority of the used NAATs over conventional culture methods for the microbiological diagnosis of PJI. Drawbacks are susceptibility to contamination in the case of 16S rRNA gene real-time PCR, labour-intensive DNA extraction and limited pathogen panel in the case of the multiplex cartridge PCR system. More prospective trials are needed to evaluate the diagnostic performance of NAATs and their impact on the clinical management of PJI. Keywords

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Section: Introductionmentioning

confidence: 99%

Diagnosis of prosthetic joint infections using UMD-Universal Kit and the automated multiplex-PCR Unyvero i60 ITI® cartridge system: a pilot study

et al. 2015

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“…Both bacteria need tooth surface for colonization. The interaction between S. sanguinis and S. mutans is affected by environmental factors, such as the density of the colony, the availability of nutrition, and pH [8]. Studies by Kreth et al showed that the interaction between S. mutans and S. sanguinis was influenced by conditions in the environment.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Effect of Java Turmeric Ethanol Extract Against Dual-Species Streptococcus Mutans and Streptococcus Sanguinis Biofilm (In Vitro)

Busri

Puspitawati

Utami

2017

Asian J Pharm Clin Res

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Objectives:The minimal bactericidal concentration (MBC) of Java turmeric (Curcuma xanthorrhiza Roxb.) ethanol extract is 25% against Streptococcus mutans and 15% against Streptococcus sanguinis as single species. This study aimed to examine the antibacterial effect of Java turmeric ethanol extract against S. mutans and S. sanguinis as dual-species. S. mutans and S. sanguinis compete against each other to obtain nutrients. Methods:The antibacterial effect of Java turmeric ethanol extract against dual-species Streptococcus in vitro was analyzed by measuring the growth of bacteria after exposure to the extract by counting colony formation and quantifying bacterial cell numbers using real-time polymerase chain reaction. Result:The MBC of Java turmeric ethanol extract against dual-species Streptococcus is 10%. S. sanguinis is more sensitive to the extract than S. mutans. Conclusions:The antibacterial effect of Java turmeric ethanol extract on S. mutans and S. sanguinis as single species differs from the effect on the bacteria as dual-species of Streptococcus.

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“…Clostridium are noted to contain either single or multiple QSS, such as: (1) LuxS, (2) Agr, (3) Agr2 that are regulated by peptide signals e.g., AIP ( Table 1) [24][25][26][27][28][29]. Table 1) [30][31][32][33][34].…”

Section: Clostridiummentioning

confidence: 99%

Potential Emergence of Multi-quorum Sensing Inhibitor Resistant (MQSIR) Bacteria

et al. 2015

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Expression of certain bacterial genes only at a high bacterial cell density is termed as quorum-sensing (QS). Here bacteria use signaling molecules to communicate among themselves. QS mediated genes are generally involved in the expression of phenotypes such as bioluminescence, biofilm formation, competence, nodulation, and virulence. QS systems (QSS) vary from a single in Vibrio spp. to multiple in Pseudomonas and Sinorhizobium species. The complexity of QSS is further enhanced by the multiplicity of signals: (1) peptides, (2) acyl-homoserine lactones, (3) diketopiperazines. To counteract this pathogenic behaviour, a wide range of bioactive molecules acting as QS inhibitors (QSIs) have been elucidated. Unlike antibiotics, QSIs don't kill bacteria and act at much lower concentration than those of antibiotics. Bacterial ability to evolve resistance against multiple drugs has cautioned researchers to develop QSIs which may not generate undue pressure on bacteria to develop resistance against them. In this paper, we have discussed the implications of the diversity and multiplicity of QSS, in acting as an arsenal to withstand attack from QSIs and may use these as reservoirs to develop multi-QSI resistance.

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Quorum Sensing and Bacterial Social Interactions in Biofilms

Cited by 559 publications

References 80 publications

Diagnosis of prosthetic joint infections using UMD-Universal Kit and the automated multiplex-PCR Unyvero i60 ITI® cartridge system: a pilot study

Diagnosis of prosthetic joint infections using UMD-Universal Kit and the automated multiplex-PCR Unyvero i60 ITI® cartridge system: a pilot study

Antibacterial Effect of Java Turmeric Ethanol Extract Against Dual-Species Streptococcus Mutans and Streptococcus Sanguinis Biofilm (In Vitro)

Potential Emergence of Multi-quorum Sensing Inhibitor Resistant (MQSIR) Bacteria

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