Quorum Sensing Activity of Hafnia alvei Isolated from  Packed Food

Tan, Jia-Yi; Yin, Wai‐Fong; Chan, Kok‐Gan

doi:10.3390/s140406788

Cited by 22 publications

(16 citation statements)

References 32 publications

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“…A report has been published that the production of C6-HSL by H. alvei 071 isolated from cooled raw milk by Viana et al [19], and 3-oxo-C8-HSL can be secreted by H. alvei FB1 as reported by Tan et al [3], while the production of C4-HSL by H. alvei was demonstrated for the first time in this study . Spoilage of food caused by bacteria is mainly associated with the production of exoenzymes that cause the degradation of food ingredients.…”

Section: Discussionsupporting

confidence: 70%

“…A positive effect on these phenotypes was observed with a supplement of C4-HSL to Aeromonas hydrophila ahyI mutant , indicating the existence of a regulatory role for C4-HSL [35]. Both H. alvei and Serratia are members of the Enterobacteriacea family, and since H. alvei and Aeromonas hydrophila are opportunistic pathogens that are also closely related, a similar regulatory function of AHLs might exist in H. alvei as mentioned by Tan et al [3]. However, this speculation needs to be verified by further study.…”

Section: Discussionmentioning

confidence: 92%

“…Moreover, food spoilage is mostly caused by bacteria, and quorum sensing (QS) is thought to play a role in bacterial-linked food spoilage and food safety [2,3,4]. Increased interest in the connection between QS and food spoilage has led to more in-depth study of the signal molecules in relation to microbes that cause food spoilage.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characteristics of N-Acylhomoserine Lactones Produced by Hafnia alvei H4 Isolated from Spoiled Instant Sea Cucumber

Hou

Zhu

Wang

et al. 2017

Sensors

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This study aimed to identify N-acylhomoserine lactone (AHL) produced by Hafnia alvei H4, which was isolated from spoiled instant sea cucumber, and to investigate the effect of AHLs on biofilm formation. Two biosensor strains, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens KYC55, were used to detect the quorum sensing (QS) activity of H. alvei H4 and to confirm the existence of AHL-mediated QS system. Thin layer chromatography (TLC) and high resolution triple quadrupole liquid chromatography/mass spectrometry (LC/MS) analysis of the AHLs extracted from the culture supernatant of H. alvei H4 revealed the existence of at least three AHLs: N-hexanoyl-l-homoserine lactone (C6-HSL), N-(3-oxo-octanoyl)-l-homoserine lactone (3-oxo-C8-HSL), and N-butyryl-l-homoserine lactone (C4-HSL). This is the first report of the production of C4-HSL by H. alvei. In order to determine the relationship between the production of AHL by H. alvei H4 and bacterial growth, the β-galactosidase assay was employed to monitor AHL activity during a 48-h growth phase. AHLs production reached a maximum level of 134.6 Miller unites at late log phase (after 18 h) and then decreased to a stable level of about 100 Miller unites. AHL production and bacterial growth displayed a similar trend, suggesting that growth of H. alvei H4 might be regulated by QS. The effect of AHLs on biofilm formation of H. alvei H4 was investigated by adding exogenous AHLs (C4-HSL, C6-HSL and 3-oxo-C8-HSL) to H. alvei H4 culture. Biofilm formation was significantly promoted (p < 0.05) by 5 and 10 µM C6-HSL, inhibited (p < 0.05) by C4-HSL (5 and 10 µM) and 5 µM 3-oxo-C8-HSL, suggesting that QS may have a regulatory role in the biofilm formation of H. alvei H4.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 92%

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Characteristics of N-Acylhomoserine Lactones Produced by Hafnia alvei H4 Isolated from Spoiled Instant Sea Cucumber

Hou

Zhu

Wang

et al. 2017

Sensors

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“…The focus of this study, H. alvei FB1 is an AHL-producing strain recovered from vacuumpacked refrigerated fish paste meatballs (commonly known as 'fish ball'). We have previously confirmed that the strain produces two oxo-AHLs with side chains of six or eight carbons in length (20).…”

Section: Introductionsupporting

confidence: 61%

Characterisation of Quorum Sensing System and Its Role in Global Regulation in Hafnia alvei

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Convey

et al. 2019

Preprint

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Quorum sensing (QS) is a regulatory process achieved via cell-to-cell communication that involves release and detection of autoinducers (AIs), and which occurs in a wide range of bacteria. To date, QS has been associated with events of pathogenesis, biofilm formation, and antibiotic resistance in clinical, industrial, and agricultural contexts. The main objective of this study was to characterise the role of N-Acyl homoserine lactone (AHL) type QS in Hafnia alvei FB1, a bacterial strain isolated from frozen vacuum-packed fish paste meatballs, via identification of QS core genes using a genomic approach, followed by comparative transcriptomic profiling between QS-deficient mutants and wild-type strains. H. alvei FB1 is known to produce two types of AHLs, namely, N-(3-oxohexanoyl) homoserine lactone (3OC6-HSL) and N-(3-oxooctanoyl) homoserine lactone (3OC8-HSL). The complete genome sequence of strain FB1 was obtained and a single gene for AHL synthase (halI) and its cognate receptor (halR) were identified. QS-deficient mutants of FB1 were constructed via the λ-Red recombineering method. Removal of the QS genes in strain FB1 affected mainly mechanisms in cell division and nutrient uptake, as well as resistance to a number of antibiotics, which are crucial for survival, adaptation and colonisation of both food and the host gut environment.Impact statement: Members of the genus Hafnia are known as opportunistic pathogens in both nosocomial and community-acquired infections. However, the involvement and mechanism of pathogenesis of Hafnia in infectious diseases remains uncertain. We investigatd the role of the signalling molecule group, N-acyl homoserine lactones (AHLs), in a Hafnia alvei strain, since AHLs play important roles in pathogenicity, survival or adaptation in other pathogens. The comparative transcriptomic study revealed that AHLs are involved in mechanisms in cell division and nutrient uptake, as well as resistance to a number of antibiotics, which are crucial for survival, adaptation and colonisation of both food and the host gut environment. These findings provide insight into possible strategies to combat this opportunistic pathogen.

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“…Acinetobacter, Brucella, Hafnia, Pantoea, Ralstonia, and Rhodobacter, are reported to possess single QSS which are regulated by AHLs signals of varying acyl chain lengths ( Table 3) [127][128][129][130][131][132][133][134]. The presence of QS has been scarcely reported in extremophiles: (1) AHL based QS in haloalkaliphiles-Natronococcus occultus [135] and Halomonas [136,137], (2) peptide-based QS in hyperthermophileThermotoga maritima [138], and (3) a QSS regulated by furanosyl-borate diester signals in archaea [139].…”

Section: Othersmentioning

confidence: 99%

Potential Emergence of Multi-quorum Sensing Inhibitor Resistant (MQSIR) Bacteria

et al. 2015

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Expression of certain bacterial genes only at a high bacterial cell density is termed as quorum-sensing (QS). Here bacteria use signaling molecules to communicate among themselves. QS mediated genes are generally involved in the expression of phenotypes such as bioluminescence, biofilm formation, competence, nodulation, and virulence. QS systems (QSS) vary from a single in Vibrio spp. to multiple in Pseudomonas and Sinorhizobium species. The complexity of QSS is further enhanced by the multiplicity of signals: (1) peptides, (2) acyl-homoserine lactones, (3) diketopiperazines. To counteract this pathogenic behaviour, a wide range of bioactive molecules acting as QS inhibitors (QSIs) have been elucidated. Unlike antibiotics, QSIs don't kill bacteria and act at much lower concentration than those of antibiotics. Bacterial ability to evolve resistance against multiple drugs has cautioned researchers to develop QSIs which may not generate undue pressure on bacteria to develop resistance against them. In this paper, we have discussed the implications of the diversity and multiplicity of QSS, in acting as an arsenal to withstand attack from QSIs and may use these as reservoirs to develop multi-QSI resistance.

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Quorum Sensing Activity of Hafnia alvei Isolated from Packed Food

Cited by 22 publications

References 32 publications

Characteristics of N-Acylhomoserine Lactones Produced by Hafnia alvei H4 Isolated from Spoiled Instant Sea Cucumber

Characteristics of N-Acylhomoserine Lactones Produced by Hafnia alvei H4 Isolated from Spoiled Instant Sea Cucumber

Characterisation of Quorum Sensing System and Its Role in Global Regulation in Hafnia alvei

Potential Emergence of Multi-quorum Sensing Inhibitor Resistant (MQSIR) Bacteria

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