Quorum sensing activity of Citrobacter amalonaticus L8A, a bacterium isolated from dental plaque

Goh, Share-Yuan; Khan, Saad Ahmed; Tee, Kok Keng; Kasim, Noor Hayaty Abu; Yin, Wai‐Fong; Chan, Kok‐Gan

doi:10.1038/srep20702

Cited by 18 publications

(20 citation statements)

References 58 publications

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“…The current accepted paradigm does not assign a significant role to AHLs in dental plaque formation since these signals are almost completely excluded from the literature reviews on QS mechanisms in oral biofilms [13][14][15][16] . Although a few strains with the ability to produce AHLs were isolated from the human tongue surface and dental plaque samples [21][22][23][24][25] , the fact that AHLs could not be detected in pure cultures of pathogenic oral bacteria in previous studies [10][11][12] is the reason why a minor role is attributed to these signals in the oral cavity. The low sensitivity of the biosensors may be partially responsible for the low number of AHL-producing strains isolated from the oral cavity.…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, several AHL-producing strains of Enterobacter sp., Klebsiella pneumoniae, Pseudomonas putida, Citrobacter amalonaticus (Levinea amalonaticus) L8A and Burkholderia sp. have been isolated from the human tongue surface and dental plaque samples [21][22][23][24][25] . Furthermore, a homologue of the AHL-synthase HdtS, as well as a LuxR-type receptor homologue, were identified in P. gingivalis W83 and P. gingivalis ATCC33277, respectively [26][27][28] .…”

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confidence: 99%

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Acyl homoserine lactone-mediated quorum sensing in the oral cavity: a paradigm revisited

Muras

Otero-Casal

Blanc

et al. 2020

Sci Rep

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Acyl homoserine lactones (AHLs), the quorum sensing (QS) signals produced by Gram-negative bacteria, are currently considered to play a minor role in the development of oral biofilm since their production by oral pathogens has not been ascertained thus far. However, we report the presence of AHLs in different oral samples and their production by the oral pathogen Porphyromonas gingivalis. the importance of AHLs is further supported by a very high prevalence of AHL-degradation capability, up to 60%, among bacteria isolated from dental plaque and saliva samples. Furthermore, the widespectrum AHL-lactonase Aii20J significantly inhibited oral biofilm formation in different in vitro biofilm models and caused important changes in bacterial composition. Besides, the inhibitory effect of Aii20J on a mixed biofilm of 6 oral pathogens was verified using confocal microscopy. Much more research is needed in order to be able to associate specific AHLs with oral pathologies and to individuate the key actors in AHL-mediated QS processes in dental plaque formation. However, these results indicate a higher relevance of the AHLs in the oral cavity than generally accepted thus far and suggest the potential use of inhibitory strategies against these signals for the prevention and treatment of oral diseases.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Acyl homoserine lactone-mediated quorum sensing in the oral cavity: a paradigm revisited

Muras

Otero-Casal

Blanc

et al. 2020

Sci Rep

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“…revealed their production of AHLs as their signalling molecules. Citrobacter amalonaticus isolated from dental plaque showed that it was able to produce several AHLs such as C8-HSL, C14-HSL and C16-HSL [ 45 ]. Similarly, a C. amalonaticus isolated from clams showed that this species was able to produce multiple types of AHLs which include short-chain AHLs such as C4-HSL, C6-HSL, C8-HSL, as well as long-chain C14-HSL and C-16 HSL [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

Green tea (Camellia sinensis) extract inhibits biofilm formation in acyl homoserine lactone-producing, antibiotic-resistant Morganella morganii isolated from Pasig River, Philippines

Guzman

Alas

Lucban

et al. 2020

Heliyon

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The drastic development of urban districts around the world has caused changes in the environment, specifically on metropolitan waterways such as the Pasig River in the Philippines. These significant changes resulted in diversity of microorganisms and their mechanisms employed such as antibiotic resistance and their communication system or quorum sensing (QS). In this study, four bacterial isolates from Pasig River, identified as Aeromonas salmonicida , Acinetobacter sp., Morganella morganii , and Citrobacter freundii , were observed to employ short-chain acyl homoserine lactone (AHL) as their signalling molecule based on in vitro assays using the biosensor strain Chromobacterium violaceum CV026. Furthermore, M. morganii isolate was shown to be resistant to chloramphenicol. This poses a significant threat not just to public health but also to the aquatic life present in the river. Thus, green tea ( Camellia sinensis ) extract was tested for its capability to inhibit in vitro biofilm formation in M. morganii , as well as the short-chain acyl homoserine lactone QS system using C. violaceum ATCC 12472. Results showed that the extract significantly ( p < 0.05) inhibited biofilm formation in M. morganii at as low as 62.5 μg/mL (31.55%). Increasing the concentration (500 μg/mL) did not significantly ( p > 0.05) enhance the activity (41.21%). Furthermore, the extract also inhibited pigmentation in C. violaceum ATCC 12472, suggesting QS inhibition. This study adds into record the production of short-chain AHLs by Aeromonas salmonicida , Acinetobacter sp., Morganella morganii , and Citrobacter freundii , as well as the potential of green tea extract as inhibitor of biofilm formation in antibiotic-resistant M. morganii possibly through QS inhibition.

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“…Identity of each isolate was evaluated and scored. Similarity scorings of these mass spectra were used for construction of a dendrogram using MALDI Biotyper MSP creation method (Bruker Daltonics, Bremen, Germany) which clustered closely related isolates and illustrated graphical distance values between them [25].…”

Section: Matrix-assisted Laser Desorption Ionization Time-of-flight (mentioning

confidence: 99%

Oecophyllibacter saccharovoransgen. nov. sp. nov., a bacterial symbiont of the weaver antOecophylla smaragdina

Chua

See-Too

Tan

et al. 2020

Preprint

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AbstractIn this study, bacterial strains Ha5T, Ta1 and Jb2 were isolated from different colonies of weaver ant Oecophylla smaragdina. They were distinguished as different strains based on matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and distinctive random-amplified polymorphic DNA (RAPD) fingerprints. Cells of these bacterial strains were Gram-negative, rod-shaped, aerobic, non-motile, catalase-positive and oxidase-negative. They were able to grow at 15–37°C (optimum, 28–30°C) and in the presence of 0–1.5 % (w/v) NaCl (optimum 0%). Their predominant cellular fatty acids were C18:1ω7c, C16:0, C19:0ω8c cyclo, C14:0 and C16:0 2-OH. Strains Ha5T, Ta1 and Jb2 shared highest 16S rRNA gene sequence similarity (94.56–94.63%) with Neokomagataea tanensis NBRC106556T but were phylogenetically closer to Bombella spp. and Saccharibacterfloricola DSM15669T. Both 16S rRNA gene sequence-based phylogenetic analysis and core gene-based phylogenomic analysis placed them in a distinct lineage in family Acetobacteraceae. These bacterial strains shared higher than species level thresholds in multiple overall genome-relatedness indices which indicated that they belonged to the same species. In addition, they did not belong to any of the current taxa of Acetobacteraceae as they had low pairwise average nucleotide identity (≤70.7%), in silico DNA-DNA hybridization (≤39.5%) and average amino acid identity (≤66.ü%) values with all the type members of the family. Based on these results, bacterial strains Ha5T, Ta1 and Jb2 represent a novel species of a novel genus in family Acetobacteraceae, for which we propose the name Oecophyllibacter saccharovorans gen. nov. sp. nov., and strain Ha5T as the type strain.

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Quorum sensing activity of Citrobacter amalonaticus L8A, a bacterium isolated from dental plaque

Cited by 18 publications

References 58 publications

Acyl homoserine lactone-mediated quorum sensing in the oral cavity: a paradigm revisited

Acyl homoserine lactone-mediated quorum sensing in the oral cavity: a paradigm revisited

Green tea (Camellia sinensis) extract inhibits biofilm formation in acyl homoserine lactone-producing, antibiotic-resistant Morganella morganii isolated from Pasig River, Philippines

Oecophyllibacter saccharovoransgen. nov. sp. nov., a bacterial symbiont of the weaver antOecophylla smaragdina

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