Quorum sensing activity and control of yeast-mycelium dimorphism in Ophiostoma floccosum

Berrocal, Alexánder; Oviedo, Claudia; Nickerson, Kenneth W.; Navarrete, José

doi:10.1007/s10529-014-1514-5

Cited by 25 publications

(16 citation statements)

References 31 publications

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“…Interestingly, other authors did not find any effect of farnesol on O. ulmi (4,26,33). In this sense, the O. piceae strain studied in this work is a saprophyte and affiliates phylogenetically with the nonpathogenic species of the Ophiostoma clade, such as O. quercus, far from the pathogens O. ulmi and O. novo-ulmi (see Fig.…”

Section: Resultsmentioning

confidence: 51%

Quorum-Sensing Mechanisms Mediated by Farnesol in Ophiostoma piceae: Effect on Secretion of Sterol Esterase

Salas

Martı́nez

Barriuso

2015

Appl Environ Microbiol

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Ophiostoma piceae CECT 20416 is a dimorphic wood-staining fungus able to produce an extracellular sterol-esterase/lipase (OPE) that is of great biotechnological interest. In this work, we have studied the morphological change of this fungus from yeast to hyphae, which is associated with the cell density-related mechanism known as quorum sensing (QS), and how this affects the secretion of OPE. The data presented here confirm that the molecule E,E-farnesol accumulates as the cell number is growing within the population. The exogenous addition of this molecule or spent medium to the cultures increased the extracellular activity of OPE 2.5 times. This fact was related not to an increase in microbial biomass or in the expression of the gene coding for OPE but to a marked morphological transition in the cultures. Moreover, the morphological transition also occurred when a high cell density was inoculated into the medium. The results suggest that E,E-farnesol regulates through QS mechanisms the morphological transition in the dimorphic fungus O. piceae and that it is associated with a higher extracellular esterase activity. Furthermore, identification and transcriptional analysis of genes tup1 and cyr1, which are involved in the response, was carried out. Here we report enhanced production of a sterol-esterase/lipase of biotechnological interest by means of QS mechanisms. These results may be useful in increasing the production of secreted enzymes of other dimorphic fungi of biotechnological interest.T riacylglycerol esterases, also known as lipases, have acylglycerols as their natural substrates, while sterol esterases hydrolyze fatty acid esters of sterols. In addition, both kinds of enzymes are able to carry out synthesis reactions in the presence of organic solvents (1, 2). They are widespread in nature, but those from microorganisms, especially fungi, have gained special interest due to their broad substrate specificity and their potential application for biotechnological applications (2).Ophiostoma piceae is a wood-staining fungus and is a causal agent of pine and spruce discoloration. Although it is not a pathogenic species and associates with nonaggressive bark beetles, the infection results in substantial economic losses in the forestry industry (3). Its ability to produce a morphological transition from yeast to hyphal forms, depending on fungal culture conditions, probably contributes to its adaptation to different environmental conditions (3). The effect of inoculum size on culture morphology of fungi from the genera Ophiostoma has been recently reported, suggesting that yeast-or hypha-like growth could be associated with quorum-sensing (QS) activity (4).The fungus O. piceae CECT 20416 produces an extracellular sterol esterase (OPE) with activity on triglycerides, esters of p-nitrophenol, and cholesterol (5). This is the only esterase secreted by this strain, and it represents a major protein in the crude enzyme obtained using a basal medium supplemented with olive oil. The enzyme is a glycosylated protein,...

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Section: Resultsmentioning

confidence: 51%

Quorum-Sensing Mechanisms Mediated by Farnesol in Ophiostoma piceae: Effect on Secretion of Sterol Esterase

Salas

Martı́nez

Barriuso

2015

Appl Environ Microbiol

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“…Similarly, Hammami et al (2010, 2011) demonstrated pseudo-hyphal growth of P. flocculosa under stress conditions as compared to yeast-like growth under control conditions (Hammami et al, 2010, 2011). In many other dimorphic fungi, the morphological choice of yeast or mycelium is associated with quorum sensing, inoculum size, stress conditions and secreted molecules (Hornby et al, 2001, 2004; Hogan, 2006; Nickerson et al, 2006; Berrocal et al, 2014). Since we did not observed coiling or the formation of long hyphae when P. aphidis interacted with B. cinerea , we assume that the interaction with powdery mildew triggers this yeast–mycelia transition, probably involving molecule secretion or other cues that activate gene expression and need to be further characterized.…”

Section: Discussionmentioning

confidence: 99%

Biological control of the cucurbit powdery mildew pathogen Podosphaera xanthii by means of the epiphytic fungus Pseudozyma aphidis and parasitism as a mode of action

et al. 2015

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Epiphytic yeasts, which colonize plant surfaces, may possess activity that can be harnessed to help plants defend themselves against various pathogens. Due to their unique characteristics, epiphytic yeasts belonging to the genus Pseudozyma hold great potential for use as biocontrol agents. We identified a unique, biologically active isolate of the epiphytic yeast Pseudozyma aphidis that is capable of inhibiting Botrytis cinerea via a dual mode of action, namely induced resistance and antibiosis. Here, we show that strain L12 of P. aphidis can reduce the severity of powdery mildew caused by Podosphaera xanthii on cucumber plants with an efficacy of 75%. Confocal and scanning electron microscopy analyses demonstrated P. aphidis proliferation on infected tissue and its production of long hyphae that parasitize the powdery mildew hyphae and spores as an ectoparasite. We also show that crude extract of P. aphidis metabolites can inhibit P. xanthii spore germination in planta. Our results suggest that in addition to its antibiosis as mode of action, P. aphidis may also act as an ectoparasite on P. xanthii. These results indicate that P. aphidis strain L12 has the potential to control powdery mildew.

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“…Dimorphism is a peculiar characteristic of several yeast species and filamentous fungi, such as Candida albicans, Saccharomyces cerevisiae, Yarrowia lypolitica, Pichia fermentans, Schizosaccharomyces japonicus, Ustilago maydis, Ophiostoma floccosum, Ceratocystis ulmi, Mycosphaerella graminicola, which can switch between unicellular yeast and multicellular filamentous growth forms in response to changing environmental cues [1][2][3][4][5][6][7][8]. Usually, in the yeast stage, mitotic divisions either by budding or fission to produce two independent cells, while in the filamentous stage, cells become elongated yet fail to abscise following cytokinesis, and remain attached to form chains of elongated pseudohyphal cells; the true hyphae are produced with long continuous tubes and septae separating each of the nuclei in these tubes [2].…”

Section: Introductionmentioning

confidence: 99%

“…Hence, in order to facilitate better control of morphology switch CONTACT Ri Ming Yan rimingyan@163.com; yanriming@jxnu.edu.cn; Du Zhu zhudu12@163.com *Both authors contributed equally to this work and further understanding of the dimorphic transitions mechanisms, a great number of investigations into the nutritional and environmental stimuli which can induce dimorphic transitions have been conducted. Previous investigations have documented that dimorphic transition is normally induced by a variety of environmental and nutritional factors including temperature [9], quorum sensing molecules [5], inoculum size [16], the accessibility of nitrogen source [14] and carbon [17], the concentration of serum [7], amino acid [18], pH [19] and so on. Despite many different environmental factors that can induce dimorphism transition, the signalling pathways involved in connecting external stimuli and cell morphological transformation are highly conserved even among distantly related fungi [14,20].…”

Section: Introductionmentioning

confidence: 99%

Influence of environmental and nutritional conditions on yeast–mycelial dimorphic transition in Trichosporon cutaneum

Zhu

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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Trichosporon cutaneum is able to metabolize high hydrophobic natural compounds such as fatty acids and also can be used as an effective biodegrader to remove a number of toxic aromatic compounds from the environment. However, its growth characteristics were poorly investigated and no yeast-mycelium dimorphism process has been established yet. In the present study, we provided first insights into the effect of nitrogen sources, carbon source, and amino acids together with pH and temperature on morphological switch of T. cutaneum B3. The results showed under close to neutral or weakly alkaline pH conditions, T. cutaneum B3 produced mostly yeast-like cells; while under acidic pH conditions, it produced mostly hyphal-like cells. Under buffered conditions, low nitrogen concentration (<0.2%) would facilitate T. cutaneum B3 to produce yeast-like cells, while the presence of relative high nitrogen concentration (>1%) would induce hyphal-like cells. Under non-buffered conditions, ammonium sulphate, diammonium phosphate, urea and Nacetylglucosamine may via alteration of environmental pH affect yeast-mycelium dimorphism transitions. Methionine, tryptophan and histidine invariably induce pseudohyphal or hyphal morphology. 25 and 28 C can promote yeast-like cells growth, while cultivated at 37 C can induce hyphal-like cells growth. Thus, the nitrogen source, alteration of environmental pH and temperature of cultivation played an important role in inducing yeast-mycelium dimorphism transition. Our study confirms the yeast-mycelium dimorphism process of T. cutaneum B3 and highlights that it seems to be a suitable yeast model for further molecular genetics investigation of dimorphism and applications in fermentation morphology engineering.

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Quorum sensing activity and control of yeast-mycelium dimorphism in Ophiostoma floccosum

Abstract: José, "Quorum sensing activity and control of yeastmycelium dimorphism in Ophiostoma floccosum" (2014). Faculty Publications in the Biological Sciences. 560.

Cited by 25 publications

References 31 publications

Quorum-Sensing Mechanisms Mediated by Farnesol in Ophiostoma piceae: Effect on Secretion of Sterol Esterase

Quorum-Sensing Mechanisms Mediated by Farnesol in Ophiostoma piceae: Effect on Secretion of Sterol Esterase

Biological control of the cucurbit powdery mildew pathogen Podosphaera xanthii by means of the epiphytic fungus Pseudozyma aphidis and parasitism as a mode of action

Influence of environmental and nutritional conditions on yeast–mycelial dimorphic transition in Trichosporon cutaneum

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