Quorum quenching effect of recombinant Paraoxonase-1 enzyme against quorum sensing genes produced from Pseudomonas aeruginosa

Faisal, Anwer Jaber; Said, Layla Abdulhamid; Ali, Munim Radwan

doi:10.1016/j.genrep.2021.101412

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Certain PONs effective against P. aeruginosa have been reported (Ozer et al, 2005;Aybey and Demirkan, 2016;Faisal et al, 2021) and considering that these PON enzymes have their origin in eukaryotic cells as mammals, could be more compatible than enzymes of prokaryotic origin for its use as therapeutics agent.…”

Section: Dioxygenases Targeting the Pqs Signalsmentioning

confidence: 99%

Quorum Quenchers as an Emerging New Strategy against <em>Pseudomonas aeruginosa</em> Infections

Valdez,

Mariela Analía,

García Contreras

et al. 2024

Preprint

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Antimicrobial resistance (AMR) poses a critical global health threat, leading to approximately 1.27 million deaths annually from infectious diseases resistant to existing antibiotics. Factors such as antibiotic misuse and environmental contamination have accelerated AMR, with the COVID-19 pandemic exacerbating the issue due to increased antibiotic usage. However, the development of new antibiotics has stagnated, with only a few approved in recent years, and many facing resistance. Among the priority pathogens identified by the WHO, Pseudomonas aeruginosa stands out as a significant concern due to its multidrug resistance and virulence. P. aeruginosa infections are challenging to treat due to intrinsic and acquired resistance mechanisms, as well as its ability to form biofilms. Additionally, P. aeruginosa produces a plethora of virulence factors, including proteases, siderophores, and toxins, further complicating treatment. Quorum sensing (QS) systems play a crucial role in regulating bacterial physiology and virulence factor expression. P. aeruginosa employs three QS systems, making it an attractive target for quorum quenching (QQ) strategies. Enzymes such as AHL lactonases, acylases, and oxidoreductases have shown promise in inhibiting QS, thus reducing virulence without impacting bacterial growth. Furthermore, natural QS inhibitors produced by various organisms offer potential therapeutic alternatives. Despite promising results in vitro, translating QQ strategies to clinical settings remains challenging. Nevertheless, QQ represents a novel approach in combating multidrug-resistant infections and warrants further investigation as a complementary therapeutic strategy alongside traditional antibiotics. This review summarizes enzymes with Qurorum Quenching activity evaluated against the pathogen P. aeruginosa and their potential applications in combination therapies.

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Section: Dioxygenases Targeting the Pqs Signalsmentioning

confidence: 99%

Quorum Quenchers as an Emerging New Strategy against <em>Pseudomonas aeruginosa</em> Infections

Valdez,

Mariela Analía,

García Contreras

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The location of the PON1 gene is between q22.1 and q21.3 on the long arm of human chromosome 7. According to the structural study using X-ray crystallography, PON1 has a six-bladed propeller structure and its central tunnel can accommodate two calcium ions 21 . PON1 (EC 3.1.8.1) is a blood-borne lactonase with a molecular weight of 43-45 kDa.…”

Section: Introductionmentioning

confidence: 99%

Inhibition Effects of Oncological Drugs for Treatment of Cancer, on Paraoxonase-1 Enzyme (PON1) Activity and Cardiovascular Toxicity

Söyüt¹

2023

International J. of Pharmacology

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Background and Objective: Paraoxonase-1 Enzyme (PON1) is a high-density lipoprotein-associated cardioprotective enzyme that prevents the formation of oxidized low-density lipoprotein. This study focused on the investigation of the in vitro inhibition effects of trastuzumab and nivolumab, which are used in the treatment of cancer, on paraoxonase-1 enzyme activity. Materials and Methods: In this study, blood samples belonging to individuals aged 7-12 years were first taken and kept for the completion of coagulation. The serum was carefully separated by centrifugation. It was then used to investigate the inhibition effects of trastuzumab and nivolumab on the separated serum paraoxonase 1. In addition, the type of inhibition between enzymes and drugs was found by using Lineweaver-Burk curves. Results: Trastuzumab and nivolumab inhibited the human serum PON1 in vitro and their IC 50 and K i values were 0.0071 and 0.0062 µM, 0.0043 and 0.0053, respectively. Trastuzumab showed competitive inhibition. Nivolumab showed non-competitive inhibition. Conclusion: This study showed that trastuzumab and nivolumab inhibit PON1 enzymatic activity. These findings suggested that a decrease in the level of PON1 enzymatic activity may contribute to the increased risk of cardiovascular toxicity.

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An insight on the powerful of bacterial quorum sensing inhibition

Naga,

Shaaban,

El-Metwally

2024

Eur J Clin Microbiol Infect Dis

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Bacteria have their own language through which they communicate with one another like all higher organisms. So, many researchers are working hard to identify and comprehend the components of this bacterial communication, known as quorum sensing (QS). In quorum sensing, bacteria use signaling molecules called autoinducers (AIs) to exchange information. Many natural compounds and extraction techniques have been intensively studied to disrupt bacterial signaling and examine their effectiveness for bacterial pathogenesis control. Quorum sensing inhibitors can interfere with QS and block the action of AI signaling molecules. Recent research indicates that quorum sensing inhibitors (QSIs) and quorum quenching enzymes (QQEs) show great promise in reducing the pathogenicity of bacteria and inhibiting biofilm synthesis. In addition, the effectiveness of QQEs and QSIs in experimental animal models was demonstrated. These are taken into account in the development of innovative medical devices, such as dressings and catheters, to prevent bacterial infections. The present review highlights this aspect with a prospective vision for its development and application.

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Quorum quenching effect of recombinant Paraoxonase-1 enzyme against quorum sensing genes produced from Pseudomonas aeruginosa

Cited by 3 publications

References 19 publications

Quorum Quenchers as an Emerging New Strategy against <em>Pseudomonas aeruginosa</em> Infections

Quorum Quenchers as an Emerging New Strategy against <em>Pseudomonas aeruginosa</em> Infections

Inhibition Effects of Oncological Drugs for Treatment of Cancer, on Paraoxonase-1 Enzyme (PON1) Activity and Cardiovascular Toxicity

An insight on the powerful of bacterial quorum sensing inhibition

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