Quorum quenching bacteria isolated from industrial wastewater sludge to control membrane biofouling

Noori, Abdolvahed; Kim, Hyeok; Kim, Myung Hee; Kim, Keum-Yong; Lee, Kibaek; Oh, Hyun‐Suk

doi:10.1016/j.biortech.2022.127077

Cited by 22 publications

(16 citation statements)

References 47 publications

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“…QQ strains, 35 which could achieve full degradation of C8-HSL anaerobically within 3 h and 30 min, respectively, AnQ-m1 seemed to have a lower QQ activity. C8-HSL of 200 nM was used as the sole carbon source for the QQ activity analyses in the two studies; 34,35 while in the current study, a mixture of five types of AHLs was used, with an initial concentration of 40 μM for each, which is 200 times as high as the mentioned studies. This significantly higher concentration of AHLs necessitates a longer degradation time.…”

Section: Resultsmentioning

confidence: 97%

Biofouling Control in an Anaerobic Membrane Bioreactor with Indigenous Quorum Quenching Bacterium Micrococcus luteus Strain AnQ-m1

Xiao

Waheed

et al. 2022

ACS EST Water

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This study isolated and screened indigenous strains quenching the acyl homoserine lactone (AHL)-mediated quorum sensing system and investigated the effectiveness of a selected strain for biofouling control in a laboratory-scale anaerobic membrane bioreactor (AnMBR). Among the 11 facultative anaerobes isolated from anaerobic sludge, Micrococcus luteus strain AnQ-m1 was screened out as the most potent AHL degrader. Its AHL degradability was further examined while encapsulating the cells in polysulfone−alginate beads to optimize the encapsulation medium recipe. The QQ beads with immobilized AnQ-m1 cells retarded the apparent membrane fouling rate by 2.2 times compared with the AnMBR with vacant beads. C8-HSL, the dominant AHL in AnMBRs, was effectively (∼70%) quenched in the QQ-AnMBR before the membrane was fouled; however, its concentration was increased to the normal level when the membrane was fouled. Polysaccharides in a loosely bound extracellular polymeric substance were gradually reduced at a constant rate during the QQ stage, while the indigenous QQ strain barely affected the microbial community structure of the bulk sludge and the water treatment performance of the AnMBR. These findings provide new insights into the QQ-based biofouling control strategy and highlight the beneficial effects of indigenous QQ strains on AnMBR stability.

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Section: Resultsmentioning

confidence: 97%

Biofouling Control in an Anaerobic Membrane Bioreactor with Indigenous Quorum Quenching Bacterium Micrococcus luteus Strain AnQ-m1

Xiao

Waheed

et al. 2022

ACS EST Water

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“…The isolation and application of QQ strains have also been frequently reported in the field of biofouling control. Noori et al (2022) have isolated two QQ strains of Bacillus spp. from the activated sludge used to treat industrial wastewater containing two toxic pollutants: tetramethylammonium hydroxide (TMAH) and 1-methyl-2-pyrrolidinone.…”

Section: Qq Enzymes and Qq Bacteria For Biofouling Controlmentioning

confidence: 99%

“…The isolation and application of QQ strains have also been frequently reported in the field of biofouling control. Noori et al. (2022) have isolated two QQ strains of Bacillus spp.…”

Section: The Application Of Qq Strategiesmentioning

confidence: 99%

Innovative microbial disease biocontrol strategies mediated by quorum quenching and their multifaceted applications: A review

et al. 2023

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With the increasing resistance exhibited by undesirable bacteria to traditional antibiotics, the need to discover alternative (or, at least, supplementary) treatments to combat chemically resistant bacteria is becoming urgent. Quorum sensing (QS) refers to a novel bacterial communication system for monitoring cell density and regulation of a network of gene expression that is mediated by a group of signaling molecules called autoinducers (AIs). QS-regulated multicellular behaviors include biofilm formation, horizontal gene transfer, and antibiotic synthesis, which are demonstrating increasing pathogenicity to plants and aquacultural animals as well as contamination of wastewater treatment devices. To inhibit QS-regulated microbial behaviors, the strategy of quorum quenching (QQ) has been developed. Different quorum quenchers interfere with QS through different mechanisms, such as competitively inhibiting AI perception (e.g., by QS inhibitors) and AI degradation (e.g., by QQ enzymes). In this review, we first introduce different signaling molecules, including diffusible signal factor (DSF) and acyl homoserine lactones (AHLs) for Gram-negative bacteria, AIPs for Gram-positive bacteria, and AI-2 for interspecies communication, thus demonstrating the mode of action of the QS system. We next exemplify the QQ mechanisms of various quorum quenchers, such as chemical QS inhibitors, and the physical/enzymatic degradation of QS signals. We devote special attention to AHL-degrading enzymes, which are categorized in detail according to their diverse catalytic mechanisms and enzymatic properties. In the final part, the applications and advantages of quorum quenchers (especially QQ enzymes and bacteria) are summarized in the context of agricultural/aquacultural pathogen biocontrol, membrane bioreactors for wastewater treatment, and the attenuation of human pathogenic bacteria. Taken together, we present the state-of-the-art in research considering QS and QQ, providing theoretical evidence and support for wider application of this promising environmentally friendly biocontrol strategy.

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“…After 24 h incubation at 37°C in the 96-well plates, CV was added and the cells were stained. The level of bio lm formed on the wells at 570 nm was quanti ed by a microplate reader (Noori et al 2022). Cells containing E. coli with the transformed pET-28a empty plasmid, sludge bacteria, and P. aeruginosa was used as controls.…”

Section: Bio Lm Microscopy Analysismentioning

confidence: 99%

“…In nature, QQ bacteria have been widely used for the prevention and treatment of QS pathogenic microorganisms and bio lms, such as Bacillus SDC-U1 (Noori et al 2022), Lactobacillus crustorum ZHG 2 − 1 (Cui et al 2020), and Bacillus safensis M146 (Yu et al 2020). These QQ bacteria achieve QS inhibition by producing enzymes with AHL degradation activity and reducing AHL signal concentrations (Bzdrenga et al 2017;Ghanei-Motlagh et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Quorum-quenching potential of recombinant PvdQ-engineered bacteria for biofilm formation

Xie

et al. 2023

Int Microbiol

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Quorum sensing (QS) is a core mechanism for bacteria to regulate bio lm formation, and therefore, QS inhibition or quorum quenching (QQ) is used as an effective and economically feasible strategy against bio lms. In this study, the PvdQ gene encoding AHL acylase was introduced into Escherichia coli (DE3), and a PvdQ-engineered bacterium with highly e cient QQ activity was obtained and used to inhibit bio lm formation. Gene sequencing and western blot analysis showed that the recombinant pET-PvdQ strain was successfully constructed. The color reaction of Agrobacterium tumefaciens A136 indicated that PvdQ engineering bacteria had shown strong AHL signal molecule quenching activity, and signi cantly inhibited the adhesion (motility) of Pseudomonas aeruginosa and bio lm formation of activated sludge bacteria in Membrane Bio-Reactor (MBR; inhibition rate 51-85%, p < 0.05). In addition, qRT-PCR testing revealed that recombinant PvdQ acylase signi cantly reduced the transcription level of QS bio lm formation related genes (cdrA, pqsA, and lasR; p < 0.05). In this study, QQ genetically engineered bacteria enhanced by genetic engineering could effectively inhibit the QS signal transduction mechanism and have the potential to control bio lm formation of pathogenic bacteria in the aquaculture environment, providing an environmentally friendly and alternative antibiotic strategy to suppress bio lm contamination.

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Quorum quenching bacteria isolated from industrial wastewater sludge to control membrane biofouling

Cited by 22 publications

References 47 publications

Biofouling Control in an Anaerobic Membrane Bioreactor with Indigenous Quorum Quenching Bacterium Micrococcus luteus Strain AnQ-m1

Biofouling Control in an Anaerobic Membrane Bioreactor with Indigenous Quorum Quenching Bacterium Micrococcus luteus Strain AnQ-m1

Innovative microbial disease biocontrol strategies mediated by quorum quenching and their multifaceted applications: A review

Quorum-quenching potential of recombinant PvdQ-engineered bacteria for biofilm formation

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