Qunantitative Determination of the Uronic Acid Composition of Alginates.

Cloning, sequence analysis and expression in Escherichia coli of a gene encoding an alginate lyase from Pseudomonas sp. OS-ALG-9

“…Alginate is an uronic acid co-polymer comprising (1-4)-linked a-L-guluronate (G) and /?-D-mannuronate (M) (Haug & Larsen, 1962). These moieties can be arranged in either homopolymeric (polyG or polyM) or heteropolymeric structures consisting of random sequences of G and M (Haug et al, 1967).…”

Additional Evidence for Substrate Specificities of Alginate Lyase Isozymes fromTurbo cornutus

“…Chemical homogeneities of M-and G-fraction were confirmed by thin layer chromatography, according to a slight modification of the procedure of Ovodov et al}) after hydrolysis with sulfuric acid in the manner described by Haug and Larsen. 9 ) The result is shown in Fig.!. Each of the preparations of the M-and Gfractions showed one main spot with a very faint one, as indicated in this chromatography, in comparison with two major spots from intact alginate which correspond to mannuronic acid and the G-fraction were digested with alginate lyase isozymes (SPI and SP2).…”

Slime Production by Pseudomonas aeruginosa

“…Haug and Larson [11] reported that intensities of the colors formed by reactions with orcinol, carbazol, and phenol-sulfuric acid vary with the kinds of uronic acids tested. According to their description, intensities of the color reactions of mannuronic acid are equal to 10%, and 80% that of glucuronic acid in terms of orcinol, carbazol, and phenolsulfuric acid tests, respectively.…”